Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0924 and BT0925 are separated by 3 nucleotides BT0925 and BT0926 overlap by 8 nucleotides
BT0924: BT0924 - conserved hypothetical protein (NCBI ptt file), at 1,137,748 to 1,138,899
BT0924
BT0925: BT0925 - ABC transporter, ATP-binding protein (NCBI ptt file), at 1,138,903 to 1,139,646
BT0925
BT0926: BT0926 - ABC-type transport, permease protein (NCBI ptt file), at 1,139,639 to 1,140,595
BT0926
Position (kb)
1138
1139
1140 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1138.061 kb on + strand, within BT0924 at 1138.318 kb on - strand, within BT0924 at 1138.371 kb on - strand, within BT0924 at 1138.602 kb on + strand, within BT0924 at 1138.742 kb on + strand, within BT0924 at 1138.842 kb on + strand at 1138.886 kb on - strand at 1138.923 kb on - strand at 1138.971 kb on - strand at 1139.044 kb on - strand, within BT0925 at 1139.170 kb on + strand, within BT0925 at 1139.273 kb on + strand, within BT0925 at 1139.312 kb on - strand, within BT0925 at 1139.323 kb on - strand, within BT0925 at 1139.427 kb on + strand, within BT0925 at 1139.510 kb on + strand, within BT0925 at 1139.522 kb on + strand, within BT0925 at 1139.740 kb on + strand, within BT0926 at 1139.740 kb on + strand, within BT0926 at 1139.746 kb on + strand, within BT0926 at 1139.773 kb on + strand, within BT0926 at 1139.836 kb on + strand, within BT0926 at 1139.989 kb on + strand, within BT0926 at 1139.996 kb on - strand, within BT0926 at 1140.035 kb on - strand, within BT0926 at 1140.180 kb on + strand, within BT0926 at 1140.181 kb on - strand, within BT0926 at 1140.257 kb on + strand, within BT0926 at 1140.258 kb on - strand, within BT0926 at 1140.450 kb on + strand, within BT0926 at 1140.538 kb on + strand at 1140.538 kb on + strand at 1140.558 kb on - strand at 1140.601 kb on + strand at 1140.602 kb on - strand at 1140.621 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 1,138,061 + BT0924 0.27 -2.9 1,138,318 - BT0924 0.49 +0.7 1,138,371 - BT0924 0.54 +0.7 1,138,602 + BT0924 0.74 -1.9 1,138,742 + BT0924 0.86 +1.7 1,138,842 + -2.3 1,138,886 - -1.9 1,138,923 - +1.4 1,138,971 - +0.4 1,139,044 - BT0925 0.19 -0.3 1,139,170 + BT0925 0.36 -1.3 1,139,273 + BT0925 0.50 +2.3 1,139,312 - BT0925 0.55 -2.3 1,139,323 - BT0925 0.56 -2.7 1,139,427 + BT0925 0.70 -1.3 1,139,510 + BT0925 0.82 -4.0 1,139,522 + BT0925 0.83 -1.9 1,139,740 + BT0926 0.11 -2.8 1,139,740 + BT0926 0.11 -2.9 1,139,746 + BT0926 0.11 -3.1 1,139,773 + BT0926 0.14 +0.7 1,139,836 + BT0926 0.21 -0.9 1,139,989 + BT0926 0.37 +0.9 1,139,996 - BT0926 0.37 -0.5 1,140,035 - BT0926 0.41 +2.6 1,140,180 + BT0926 0.57 -3.0 1,140,181 - BT0926 0.57 -2.5 1,140,257 + BT0926 0.65 -2.8 1,140,258 - BT0926 0.65 -2.7 1,140,450 + BT0926 0.85 -0.8 1,140,538 + +0.7 1,140,538 + +1.8 1,140,558 - -0.8 1,140,601 + -0.3 1,140,602 - +0.5 1,140,621 + +2.2
Or see this region's nucleotide sequence