Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0780 and BT0781 are separated by 4 nucleotides BT0781 and BT0782 are separated by 127 nucleotides BT0782 and BT0783 overlap by 52 nucleotides BT0783 and BT0784 are separated by 278 nucleotides BT0784 and BT0785 are separated by 181 nucleotides
BT0780: BT0780 - acetyltransferase (NCBI ptt file), at 971,212 to 971,718
BT0780
BT0781: BT0781 - hypothetical protein (NCBI ptt file), at 971,723 to 972,445
BT0781
BT0782: BT0782 - hypothetical protein (NCBI ptt file), at 972,573 to 972,776
BT0782
BT0783: BT0783 - hypothetical protein (NCBI ptt file), at 972,725 to 972,913
BT0783
BT0784: BT0784 - putative RNA-binding protein (NCBI ptt file), at 973,192 to 973,437
BT0784
BT0785: BT0785 - glutamine synthetase I (NCBI ptt file), at 973,619 to 975,121
BT0785
Position (kb) 972
973 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 971.610 kb on - strand, within BT0780 at 971.672 kb on - strand at 971.718 kb on + strand at 971.809 kb on - strand, within BT0781 at 971.809 kb on - strand, within BT0781 at 971.819 kb on - strand, within BT0781 at 971.840 kb on - strand, within BT0781 at 971.851 kb on + strand, within BT0781 at 971.852 kb on - strand, within BT0781 at 971.865 kb on + strand, within BT0781 at 971.874 kb on - strand, within BT0781 at 971.874 kb on - strand, within BT0781 at 971.984 kb on - strand, within BT0781 at 971.996 kb on - strand, within BT0781 at 972.032 kb on + strand, within BT0781 at 972.033 kb on - strand, within BT0781 at 972.088 kb on + strand, within BT0781 at 972.112 kb on - strand, within BT0781 at 972.159 kb on - strand, within BT0781 at 972.178 kb on + strand, within BT0781 at 972.191 kb on + strand, within BT0781 at 972.247 kb on + strand, within BT0781 at 972.248 kb on - strand, within BT0781 at 972.293 kb on - strand, within BT0781 at 972.319 kb on + strand, within BT0781 at 972.378 kb on - strand at 972.458 kb on + strand at 972.459 kb on - strand at 972.667 kb on - strand, within BT0782 at 972.704 kb on + strand, within BT0782 at 972.742 kb on + strand at 972.743 kb on - strand at 972.743 kb on - strand at 972.767 kb on + strand at 972.786 kb on - strand, within BT0783 at 972.856 kb on + strand, within BT0783 at 972.857 kb on - strand, within BT0783 at 972.914 kb on + strand at 972.956 kb on + strand at 973.135 kb on + strand at 973.219 kb on + strand, within BT0784 at 973.221 kb on + strand, within BT0784 at 973.457 kb on + strand at 973.461 kb on + strand at 973.723 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 971,610 - BT0780 0.79 +2.7 971,672 - -4.6 971,718 + -1.9 971,809 - BT0781 0.12 -0.3 971,809 - BT0781 0.12 -0.3 971,819 - BT0781 0.13 +1.0 971,840 - BT0781 0.16 -1.1 971,851 + BT0781 0.18 -2.0 971,852 - BT0781 0.18 +1.3 971,865 + BT0781 0.20 -0.6 971,874 - BT0781 0.21 -1.6 971,874 - BT0781 0.21 +0.7 971,984 - BT0781 0.36 +0.7 971,996 - BT0781 0.38 +0.7 972,032 + BT0781 0.43 +1.1 972,033 - BT0781 0.43 -3.1 972,088 + BT0781 0.50 -3.1 972,112 - BT0781 0.54 -0.7 972,159 - BT0781 0.60 +1.7 972,178 + BT0781 0.63 +1.3 972,191 + BT0781 0.65 +0.1 972,247 + BT0781 0.72 -1.3 972,248 - BT0781 0.73 -0.3 972,293 - BT0781 0.79 -2.0 972,319 + BT0781 0.82 +0.3 972,378 - -1.5 972,458 + +2.6 972,459 - -0.3 972,667 - BT0782 0.46 +1.4 972,704 + BT0782 0.64 +2.6 972,742 + +0.2 972,743 - -0.3 972,743 - -0.3 972,767 + -0.3 972,786 - BT0783 0.32 -3.5 972,856 + BT0783 0.69 -0.8 972,857 - BT0783 0.70 +3.2 972,914 + -1.3 972,956 + +1.0 973,135 + -4.5 973,219 + BT0784 0.11 -0.3 973,221 + BT0784 0.12 -4.0 973,457 + -2.3 973,461 + -0.0 973,723 + -2.0
Or see this region's nucleotide sequence