Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0436 and BT0437 are separated by 37 nucleotides BT0437 and BT0438 are separated by 48 nucleotides
BT0436: BT0436 - arabinose-proton symporter (NCBI ptt file), at 524,995 to 526,401
BT0436
BT0437: BT0437 - N-acylglucosamine 2-epimerase (NCBI ptt file), at 526,439 to 527,629
BT0437
BT0438: BT0438 - alpha-N-acetylglucosaminidase precursor (NCBI ptt file), at 527,678 to 529,870
BT0438
Position (kb) 526
527
528 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 525.505 kb on - strand, within BT0436 at 525.842 kb on + strand, within BT0436 at 525.847 kb on + strand, within BT0436 at 525.848 kb on - strand, within BT0436 at 526.430 kb on + strand at 526.437 kb on - strand at 526.775 kb on - strand, within BT0437 at 526.912 kb on + strand, within BT0437 at 526.913 kb on - strand, within BT0437 at 527.081 kb on - strand, within BT0437 at 527.512 kb on - strand at 527.557 kb on - strand at 527.599 kb on - strand at 527.618 kb on - strand at 527.650 kb on - strand at 527.653 kb on - strand at 527.696 kb on + strand at 527.696 kb on + strand at 527.696 kb on + strand at 527.704 kb on - strand at 527.927 kb on + strand, within BT0438 at 527.964 kb on - strand, within BT0438 at 527.997 kb on + strand, within BT0438 at 528.077 kb on - strand, within BT0438 at 528.132 kb on + strand, within BT0438 at 528.142 kb on + strand, within BT0438 at 528.178 kb on - strand, within BT0438 at 528.381 kb on + strand, within BT0438 at 528.403 kb on - strand, within BT0438 at 528.467 kb on + strand, within BT0438 at 528.560 kb on + strand, within BT0438 at 528.561 kb on - strand, within BT0438 at 528.596 kb on - strand, within BT0438
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 525,505 - BT0436 0.36 -1.3 525,842 + BT0436 0.60 +0.7 525,847 + BT0436 0.61 +1.0 525,848 - BT0436 0.61 +0.3 526,430 + +1.7 526,437 - +2.7 526,775 - BT0437 0.28 -0.9 526,912 + BT0437 0.40 -3.1 526,913 - BT0437 0.40 -3.3 527,081 - BT0437 0.54 -0.3 527,512 - -1.3 527,557 - -1.1 527,599 - -2.6 527,618 - -2.3 527,650 - -0.3 527,653 - -1.6 527,696 + -0.3 527,696 + +3.5 527,696 + -4.5 527,704 - -0.3 527,927 + BT0438 0.11 -2.1 527,964 - BT0438 0.13 +1.6 527,997 + BT0438 0.15 -3.3 528,077 - BT0438 0.18 -1.6 528,132 + BT0438 0.21 -2.9 528,142 + BT0438 0.21 -2.3 528,178 - BT0438 0.23 +2.3 528,381 + BT0438 0.32 -0.3 528,403 - BT0438 0.33 -0.3 528,467 + BT0438 0.36 -2.3 528,560 + BT0438 0.40 -1.4 528,561 - BT0438 0.40 -1.3 528,596 - BT0438 0.42 -1.3
Or see this region's nucleotide sequence