Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0394 and BT0395 are separated by 170 nucleotides BT0395 and BT0396 are separated by 182 nucleotides BT0396 and BT0397 are separated by 5 nucleotides BT0397 and BT0398 are separated by 41 nucleotides
BT0394: BT0394 - capsular polysaccharide biosynthesis glycosyltransferase (NCBI ptt file), at 483,453 to 484,547
BT0394
BT0395: BT0395 - hypothetical protein (NCBI ptt file), at 484,718 to 484,945
BT0395
BT0396: BT0396 - putative UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI ptt file), at 485,128 to 486,117
BT0396
BT0397: BT0397 - conserved hypothetical protein (NCBI ptt file), at 486,123 to 486,569
BT0397
BT0398: BT0398 - polysaccharide export outer membrane protein (NCBI ptt file), at 486,611 to 487,405
BT0398
Position (kb) 485
486
487 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 484.152 kb on - strand, within BT0394 at 484.281 kb on - strand, within BT0394 at 484.285 kb on + strand, within BT0394 at 484.521 kb on + strand at 484.678 kb on + strand at 484.701 kb on + strand at 484.776 kb on - strand, within BT0395 at 484.810 kb on - strand, within BT0395 at 484.814 kb on + strand, within BT0395 at 484.830 kb on + strand, within BT0395 at 484.831 kb on - strand, within BT0395 at 484.850 kb on - strand, within BT0395 at 484.870 kb on + strand, within BT0395 at 485.082 kb on - strand at 485.104 kb on - strand at 485.202 kb on + strand at 485.249 kb on + strand, within BT0396 at 485.339 kb on + strand, within BT0396 at 485.448 kb on + strand, within BT0396 at 485.477 kb on + strand, within BT0396 at 485.478 kb on - strand, within BT0396 at 485.480 kb on + strand, within BT0396 at 485.480 kb on + strand, within BT0396 at 485.498 kb on - strand, within BT0396 at 485.520 kb on - strand, within BT0396 at 485.526 kb on + strand, within BT0396 at 485.564 kb on + strand, within BT0396 at 485.592 kb on + strand, within BT0396 at 485.628 kb on + strand, within BT0396 at 485.802 kb on - strand, within BT0396 at 485.925 kb on - strand, within BT0396 at 485.940 kb on + strand, within BT0396 at 486.028 kb on - strand at 486.049 kb on - strand at 486.084 kb on - strand at 486.305 kb on - strand, within BT0397 at 486.463 kb on + strand, within BT0397 at 486.534 kb on + strand at 486.693 kb on - strand, within BT0398 at 486.693 kb on - strand, within BT0398 at 486.714 kb on + strand, within BT0398 at 486.717 kb on + strand, within BT0398 at 486.720 kb on - strand, within BT0398 at 486.784 kb on - strand, within BT0398 at 486.793 kb on + strand, within BT0398 at 486.836 kb on - strand, within BT0398 at 486.847 kb on + strand, within BT0398 at 486.891 kb on - strand, within BT0398 at 487.015 kb on - strand, within BT0398
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 484,152 - BT0394 0.64 -1.9 484,281 - BT0394 0.76 +2.1 484,285 + BT0394 0.76 -1.7 484,521 + -0.3 484,678 + -1.3 484,701 + -2.3 484,776 - BT0395 0.25 +1.5 484,810 - BT0395 0.40 +0.3 484,814 + BT0395 0.42 +0.7 484,830 + BT0395 0.49 +1.3 484,831 - BT0395 0.50 -1.5 484,850 - BT0395 0.58 -0.8 484,870 + BT0395 0.67 -0.3 485,082 - +2.5 485,104 - +1.3 485,202 + -2.3 485,249 + BT0396 0.12 -1.3 485,339 + BT0396 0.21 -2.9 485,448 + BT0396 0.32 -1.8 485,477 + BT0396 0.35 -1.9 485,478 - BT0396 0.35 -3.1 485,480 + BT0396 0.36 +1.7 485,480 + BT0396 0.36 -2.3 485,498 - BT0396 0.37 -2.5 485,520 - BT0396 0.40 -1.9 485,526 + BT0396 0.40 -0.3 485,564 + BT0396 0.44 -2.6 485,592 + BT0396 0.47 +0.7 485,628 + BT0396 0.51 -2.9 485,802 - BT0396 0.68 -1.3 485,925 - BT0396 0.81 -0.3 485,940 + BT0396 0.82 -0.9 486,028 - +0.8 486,049 - -0.3 486,084 - +2.4 486,305 - BT0397 0.41 -1.9 486,463 + BT0397 0.76 +1.7 486,534 + -2.6 486,693 - BT0398 0.10 -1.9 486,693 - BT0398 0.10 -0.9 486,714 + BT0398 0.13 +1.3 486,717 + BT0398 0.13 +2.1 486,720 - BT0398 0.14 +1.7 486,784 - BT0398 0.22 -2.6 486,793 + BT0398 0.23 -2.3 486,836 - BT0398 0.28 -1.9 486,847 + BT0398 0.30 +0.1 486,891 - BT0398 0.35 +1.7 487,015 - BT0398 0.51 -3.1
Or see this region's nucleotide sequence