Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0376 and BT0377 are separated by 6 nucleotides BT0377 and BT0378 are separated by 53 nucleotides BT0378 and BT0379 are separated by 34 nucleotides
BT0376: BT0376 - putative transcriptional regulator (NCBI ptt file), at 463,409 to 463,987
BT0376
BT0377: BT0377 - conserved hypothetical protein (NCBI ptt file), at 463,994 to 464,353
BT0377
BT0378: BT0378 - capsular polysaccharide biosynthesis protein capD (NCBI ptt file), at 464,407 to 466,332
BT0378
BT0379: BT0379 - UDP-glucose 6-dehydrogenase (NCBI ptt file), at 466,367 to 467,683
BT0379
Position (kb) 464
465
466
467 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 463.491 kb on - strand, within BT0376 at 463.704 kb on - strand, within BT0376 at 463.921 kb on + strand, within BT0376 at 463.962 kb on + strand at 463.963 kb on - strand at 463.986 kb on - strand at 464.089 kb on - strand, within BT0377 at 464.148 kb on - strand, within BT0377 at 464.575 kb on - strand at 464.638 kb on - strand, within BT0378 at 464.948 kb on - strand, within BT0378 at 464.957 kb on + strand, within BT0378 at 464.958 kb on - strand, within BT0378 at 465.025 kb on - strand, within BT0378 at 465.550 kb on - strand, within BT0378 at 465.550 kb on - strand, within BT0378 at 466.331 kb on - strand at 466.340 kb on - strand at 466.526 kb on + strand, within BT0379 at 466.617 kb on + strand, within BT0379 at 466.627 kb on + strand, within BT0379 at 466.628 kb on - strand, within BT0379 at 466.671 kb on + strand, within BT0379 at 466.726 kb on - strand at 466.726 kb on - strand, within BT0379 at 466.732 kb on + strand, within BT0379 at 466.855 kb on - strand, within BT0379 at 466.856 kb on + strand, within BT0379 at 467.036 kb on - strand, within BT0379 at 467.038 kb on - strand, within BT0379 at 467.068 kb on + strand, within BT0379 at 467.071 kb on + strand, within BT0379 at 467.072 kb on - strand, within BT0379 at 467.097 kb on - strand, within BT0379 at 467.113 kb on + strand, within BT0379 at 467.154 kb on + strand, within BT0379
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 463,491 - BT0376 0.14 +0.3 463,704 - BT0376 0.51 -4.1 463,921 + BT0376 0.88 -2.6 463,962 + +2.3 463,963 - -1.8 463,986 - -2.8 464,089 - BT0377 0.26 -1.3 464,148 - BT0377 0.43 -0.3 464,575 - +0.7 464,638 - BT0378 0.12 -1.8 464,948 - BT0378 0.28 -1.3 464,957 + BT0378 0.29 -2.8 464,958 - BT0378 0.29 -2.6 465,025 - BT0378 0.32 -0.3 465,550 - BT0378 0.59 +0.7 465,550 - BT0378 0.59 -3.4 466,331 - -2.3 466,340 - -2.6 466,526 + BT0379 0.12 -1.1 466,617 + BT0379 0.19 +2.3 466,627 + BT0379 0.20 +2.1 466,628 - BT0379 0.20 +2.5 466,671 + BT0379 0.23 +1.3 466,726 - -1.3 466,726 - BT0379 0.27 -0.3 466,732 + BT0379 0.28 +2.9 466,855 - BT0379 0.37 -1.3 466,856 + BT0379 0.37 +0.6 467,036 - BT0379 0.51 -2.3 467,038 - BT0379 0.51 +0.1 467,068 + BT0379 0.53 +2.3 467,071 + BT0379 0.53 +2.3 467,072 - BT0379 0.54 +0.2 467,097 - BT0379 0.55 +0.7 467,113 + BT0379 0.57 -0.8 467,154 + BT0379 0.60 -1.3
Or see this region's nucleotide sequence