Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0191 and BT0192 are separated by 27 nucleotides BT0192 and BT0193 are separated by 6 nucleotides BT0193 and BT0194 are separated by 1 nucleotides
BT0191: BT0191 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 203,588 to 204,979
BT0191
BT0192: BT0192 - cell surface protein (NCBI ptt file), at 205,007 to 206,974
BT0192
BT0193: BT0193 - conserved hypothetical protein (NCBI ptt file), at 206,981 to 207,862
BT0193
BT0194: BT0194 - hypothetical protein (NCBI ptt file), at 207,864 to 208,922
BT0194
Position (kb) 205
206
207 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 204.032 kb on - strand, within BT0191 at 204.036 kb on - strand, within BT0191 at 204.169 kb on + strand, within BT0191 at 204.301 kb on - strand, within BT0191 at 204.309 kb on - strand, within BT0191 at 204.337 kb on - strand, within BT0191 at 204.434 kb on - strand, within BT0191 at 204.529 kb on - strand, within BT0191 at 204.586 kb on + strand, within BT0191 at 204.586 kb on + strand, within BT0191 at 204.608 kb on - strand, within BT0191 at 204.711 kb on + strand, within BT0191 at 204.866 kb on - strand at 204.884 kb on - strand at 204.914 kb on - strand at 204.914 kb on - strand at 205.190 kb on - strand at 205.193 kb on - strand at 205.525 kb on + strand, within BT0192 at 205.631 kb on - strand, within BT0192 at 205.689 kb on - strand, within BT0192 at 205.691 kb on - strand, within BT0192 at 205.696 kb on + strand, within BT0192 at 205.750 kb on + strand, within BT0192 at 205.804 kb on + strand, within BT0192 at 205.813 kb on + strand, within BT0192 at 205.829 kb on - strand, within BT0192 at 205.879 kb on + strand, within BT0192 at 205.971 kb on - strand, within BT0192 at 205.971 kb on - strand, within BT0192 at 205.973 kb on - strand, within BT0192 at 206.054 kb on - strand at 206.064 kb on - strand, within BT0192 at 206.098 kb on + strand, within BT0192 at 206.099 kb on - strand, within BT0192 at 206.146 kb on - strand, within BT0192 at 206.351 kb on - strand, within BT0192 at 206.351 kb on - strand, within BT0192 at 206.375 kb on - strand, within BT0192 at 206.471 kb on + strand, within BT0192 at 206.578 kb on - strand, within BT0192 at 206.628 kb on - strand, within BT0192 at 206.688 kb on - strand, within BT0192 at 206.707 kb on + strand, within BT0192 at 206.708 kb on - strand, within BT0192 at 206.762 kb on - strand, within BT0192 at 206.875 kb on + strand at 206.947 kb on + strand at 207.430 kb on + strand, within BT0193 at 207.430 kb on + strand, within BT0193 at 207.527 kb on - strand, within BT0193 at 207.619 kb on - strand, within BT0193 at 207.640 kb on - strand, within BT0193 at 207.741 kb on - strand, within BT0193 at 207.846 kb on - strand at 207.848 kb on - strand at 207.861 kb on - strand at 207.863 kb on + strand at 207.950 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 204,032 - BT0191 0.32 -2.7 204,036 - BT0191 0.32 +1.1 204,169 + BT0191 0.42 +1.4 204,301 - BT0191 0.51 -2.8 204,309 - BT0191 0.52 -1.3 204,337 - BT0191 0.54 +2.4 204,434 - BT0191 0.61 -2.0 204,529 - BT0191 0.68 -1.8 204,586 + BT0191 0.72 -1.8 204,586 + BT0191 0.72 -2.8 204,608 - BT0191 0.73 +1.3 204,711 + BT0191 0.81 -0.2 204,866 - -3.5 204,884 - -1.2 204,914 - +1.6 204,914 - -1.2 205,190 - +2.8 205,193 - +0.8 205,525 + BT0192 0.26 -0.2 205,631 - BT0192 0.32 +2.1 205,689 - BT0192 0.35 -2.6 205,691 - BT0192 0.35 -1.5 205,696 + BT0192 0.35 -2.2 205,750 + BT0192 0.38 -0.8 205,804 + BT0192 0.40 -1.2 205,813 + BT0192 0.41 -2.5 205,829 - BT0192 0.42 -1.6 205,879 + BT0192 0.44 -0.2 205,971 - BT0192 0.49 -1.8 205,971 - BT0192 0.49 +2.6 205,973 - BT0192 0.49 -0.1 206,054 - -0.2 206,064 - BT0192 0.54 +1.8 206,098 + BT0192 0.55 -1.5 206,099 - BT0192 0.55 -2.4 206,146 - BT0192 0.58 +3.6 206,351 - BT0192 0.68 -1.0 206,351 - BT0192 0.68 -4.3 206,375 - BT0192 0.70 +0.8 206,471 + BT0192 0.74 -2.8 206,578 - BT0192 0.80 +2.8 206,628 - BT0192 0.82 -0.2 206,688 - BT0192 0.85 -3.1 206,707 + BT0192 0.86 -1.2 206,708 - BT0192 0.86 +0.3 206,762 - BT0192 0.89 -1.2 206,875 + -3.9 206,947 + -2.2 207,430 + BT0193 0.51 +0.2 207,430 + BT0193 0.51 -0.2 207,527 - BT0193 0.62 -0.4 207,619 - BT0193 0.72 -0.2 207,640 - BT0193 0.75 -2.5 207,741 - BT0193 0.86 -2.2 207,846 - -3.0 207,848 - +0.1 207,861 - -2.8 207,863 + +0.8 207,950 + -1.7
Or see this region's nucleotide sequence