Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0178 and BT0179 are separated by 9 nucleotides BT0179 and BT0180 are separated by 89 nucleotides BT0180 and BT0181 are separated by 12 nucleotides BT0181 and BT0182 are separated by 193 nucleotides
BT0178: BT0178 - hypothetical protein (NCBI ptt file), at 186,456 to 187,001
BT0178
BT0179: BT0179 - SsrA-binding protein (NCBI ptt file), at 187,011 to 187,463
BT0179
BT0180: BT0180 - 5-methyltetrahydrofolate-homocysteine methyltransferase (NCBI ptt file), at 187,553 to 190,300
BT0180
BT0181: BT0181 - putative NADH dehydrogenase/NAD(P)H nitroreductase (NCBI ptt file), at 190,313 to 190,912
BT0181
BT0182: BT0182 - osmo-regulated proline transporter (Na+/proline symporter) (NCBI ptt file), at 191,106 to 192,656
BT0182
Position (kb) 187
188
189
190
191 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 186.625 kb on + strand, within BT0178 at 186.695 kb on + strand, within BT0178 at 186.855 kb on + strand, within BT0178 at 186.943 kb on + strand, within BT0178 at 186.946 kb on + strand, within BT0178 at 186.946 kb on + strand, within BT0178 at 186.991 kb on + strand at 186.999 kb on + strand at 187.480 kb on - strand at 187.502 kb on - strand at 187.512 kb on + strand at 187.540 kb on + strand at 187.541 kb on - strand at 187.632 kb on - strand at 187.632 kb on - strand at 187.676 kb on - strand at 187.742 kb on - strand at 187.789 kb on + strand at 187.838 kb on + strand, within BT0180 at 187.895 kb on + strand, within BT0180 at 187.948 kb on + strand, within BT0180 at 187.949 kb on - strand, within BT0180 at 188.141 kb on - strand, within BT0180 at 188.157 kb on + strand, within BT0180 at 188.157 kb on + strand, within BT0180 at 188.158 kb on - strand, within BT0180 at 188.207 kb on - strand, within BT0180 at 188.207 kb on - strand, within BT0180 at 188.261 kb on - strand, within BT0180 at 188.345 kb on + strand, within BT0180 at 188.362 kb on + strand, within BT0180 at 188.363 kb on - strand, within BT0180 at 188.363 kb on - strand, within BT0180 at 188.438 kb on - strand, within BT0180 at 188.492 kb on + strand, within BT0180 at 188.493 kb on - strand, within BT0180 at 188.560 kb on + strand, within BT0180 at 188.618 kb on - strand, within BT0180 at 188.619 kb on + strand, within BT0180 at 188.681 kb on + strand, within BT0180 at 188.744 kb on - strand, within BT0180 at 189.037 kb on - strand, within BT0180 at 189.170 kb on - strand, within BT0180 at 189.263 kb on + strand, within BT0180 at 189.266 kb on - strand, within BT0180 at 189.543 kb on - strand, within BT0180 at 189.611 kb on - strand, within BT0180 at 189.618 kb on + strand, within BT0180 at 189.739 kb on + strand, within BT0180 at 189.739 kb on + strand, within BT0180 at 189.739 kb on + strand, within BT0180 at 189.740 kb on - strand, within BT0180 at 189.881 kb on - strand, within BT0180 at 189.882 kb on + strand, within BT0180 at 190.075 kb on + strand at 190.075 kb on + strand at 190.076 kb on - strand at 190.099 kb on - strand at 190.100 kb on + strand at 190.454 kb on - strand, within BT0181 at 190.454 kb on - strand, within BT0181 at 190.630 kb on + strand, within BT0181 at 190.640 kb on - strand, within BT0181 at 190.676 kb on + strand, within BT0181 at 190.677 kb on - strand, within BT0181 at 190.796 kb on - strand, within BT0181 at 190.938 kb on - strand at 191.020 kb on + strand at 191.037 kb on - strand at 191.080 kb on - strand at 191.111 kb on + strand at 191.142 kb on + strand at 191.181 kb on - strand at 191.206 kb on - strand at 191.262 kb on - strand, within BT0182 at 191.268 kb on - strand, within BT0182 at 191.283 kb on - strand, within BT0182 at 191.284 kb on + strand, within BT0182 at 191.284 kb on + strand, within BT0182
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 186,625 + BT0178 0.31 +0.8 186,695 + BT0178 0.44 +3.3 186,855 + BT0178 0.73 +1.8 186,943 + BT0178 0.89 +0.4 186,946 + BT0178 0.90 -2.2 186,946 + BT0178 0.90 +0.8 186,991 + +0.8 186,999 + +0.8 187,480 - -2.2 187,502 - -4.0 187,512 + +0.4 187,540 + -3.6 187,541 - -4.1 187,632 - -4.7 187,632 - -4.9 187,676 - -0.2 187,742 - -2.5 187,789 + -2.8 187,838 + BT0180 0.10 +0.8 187,895 + BT0180 0.12 -1.2 187,948 + BT0180 0.14 -0.2 187,949 - BT0180 0.14 -3.6 188,141 - BT0180 0.21 -4.0 188,157 + BT0180 0.22 -1.2 188,157 + BT0180 0.22 -5.6 188,158 - BT0180 0.22 -3.6 188,207 - BT0180 0.24 -4.4 188,207 - BT0180 0.24 -4.5 188,261 - BT0180 0.26 -4.6 188,345 + BT0180 0.29 -1.8 188,362 + BT0180 0.29 -1.8 188,363 - BT0180 0.29 -4.1 188,363 - BT0180 0.29 -3.5 188,438 - BT0180 0.32 -1.8 188,492 + BT0180 0.34 -2.2 188,493 - BT0180 0.34 -0.2 188,560 + BT0180 0.37 -2.2 188,618 - BT0180 0.39 -1.2 188,619 + BT0180 0.39 -3.2 188,681 + BT0180 0.41 -4.0 188,744 - BT0180 0.43 -1.5 189,037 - BT0180 0.54 -3.1 189,170 - BT0180 0.59 -2.4 189,263 + BT0180 0.62 -2.5 189,266 - BT0180 0.62 -1.8 189,543 - BT0180 0.72 -4.7 189,611 - BT0180 0.75 -2.2 189,618 + BT0180 0.75 -1.2 189,739 + BT0180 0.80 -3.1 189,739 + BT0180 0.80 -1.2 189,739 + BT0180 0.80 -1.2 189,740 - BT0180 0.80 -3.7 189,881 - BT0180 0.85 -0.2 189,882 + BT0180 0.85 -1.2 190,075 + -2.2 190,075 + -3.5 190,076 - -0.8 190,099 - -0.2 190,100 + -0.2 190,454 - BT0181 0.23 -0.5 190,454 - BT0181 0.23 -1.2 190,630 + BT0181 0.53 -0.2 190,640 - BT0181 0.55 +1.4 190,676 + BT0181 0.60 -1.2 190,677 - BT0181 0.61 +2.1 190,796 - BT0181 0.81 -0.9 190,938 - +0.6 191,020 + +2.8 191,037 - -2.4 191,080 - -2.5 191,111 + -0.8 191,142 + +0.2 191,181 - -0.7 191,206 - +2.1 191,262 - BT0182 0.10 -1.2 191,268 - BT0182 0.10 -2.3 191,283 - BT0182 0.11 +0.1 191,284 + BT0182 0.11 -1.8 191,284 + BT0182 0.11 -3.0
Or see this region's nucleotide sequence