Experiment: BHIS with Trimethoprim 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0059 and BT0060 are separated by 38 nucleotides BT0060 and BT0061 are separated by 15 nucleotides BT0061 and BT0062 overlap by 4 nucleotides BT0062 and BT0063 are separated by 52 nucleotides BT0063 and BT0064 are separated by 20 nucleotides
BT0059: BT0059 - hypothetical protein (NCBI ptt file), at 54,774 to 55,715
BT0059
BT0060: BT0060 - putative polysaccharide export protein (NCBI ptt file), at 55,754 to 56,539
BT0060
BT0061: BT0061 - putative tyrosine-protein kinase ptk (NCBI ptt file), at 56,555 to 58,894
BT0061
BT0062: BT0062 - hypothetical protein (NCBI ptt file), at 58,891 to 59,103
BT0062
BT0063: BT0063 - hypothetical protein (NCBI ptt file), at 59,156 to 59,356
BT0063
BT0064: BT0064 - hypothetical protein (NCBI ptt file), at 59,377 to 61,551
BT0064
Position (kb) 56
57
58
59 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 55.735 kb on - strand at 55.884 kb on - strand, within BT0060 at 56.059 kb on + strand, within BT0060 at 56.151 kb on - strand, within BT0060 at 56.403 kb on - strand, within BT0060 at 56.426 kb on + strand, within BT0060 at 56.786 kb on + strand at 56.786 kb on + strand at 56.787 kb on - strand at 56.920 kb on + strand, within BT0061 at 56.921 kb on - strand, within BT0061 at 57.053 kb on + strand, within BT0061 at 57.308 kb on + strand, within BT0061 at 57.308 kb on + strand, within BT0061 at 57.309 kb on - strand, within BT0061 at 57.309 kb on - strand, within BT0061 at 57.477 kb on - strand, within BT0061 at 57.749 kb on - strand, within BT0061 at 58.625 kb on + strand, within BT0061 at 59.155 kb on + strand at 59.285 kb on - strand, within BT0063 at 59.330 kb on - strand, within BT0063 at 59.351 kb on - strand at 59.361 kb on + strand at 59.362 kb on - strand at 59.362 kb on - strand at 59.460 kb on + strand at 59.461 kb on - strand at 59.490 kb on + strand at 59.599 kb on + strand, within BT0064 at 59.624 kb on - strand, within BT0064 at 59.624 kb on - strand, within BT0064 at 59.624 kb on - strand, within BT0064 at 59.624 kb on - strand, within BT0064 at 59.624 kb on - strand, within BT0064 at 59.624 kb on - strand, within BT0064 at 59.629 kb on + strand, within BT0064 at 59.668 kb on + strand, within BT0064 at 59.669 kb on - strand, within BT0064 at 59.767 kb on + strand, within BT0064
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 0.00625 mM remove 55,735 - -1.8 55,884 - BT0060 0.17 -3.3 56,059 + BT0060 0.39 +0.9 56,151 - BT0060 0.51 -1.6 56,403 - BT0060 0.83 -0.1 56,426 + BT0060 0.85 -0.1 56,786 + +2.7 56,786 + -1.1 56,787 - -0.1 56,920 + BT0061 0.16 -2.3 56,921 - BT0061 0.16 +1.7 57,053 + BT0061 0.21 +1.9 57,308 + BT0061 0.32 -1.1 57,308 + BT0061 0.32 -3.7 57,309 - BT0061 0.32 -2.1 57,309 - BT0061 0.32 -4.8 57,477 - BT0061 0.39 -2.7 57,749 - BT0061 0.51 +1.4 58,625 + BT0061 0.88 -1.7 59,155 + -2.7 59,285 - BT0063 0.64 -1.1 59,330 - BT0063 0.87 -0.6 59,351 - -1.1 59,361 + -1.1 59,362 - -0.5 59,362 - -3.2 59,460 + -0.9 59,461 - -2.9 59,490 + -0.7 59,599 + BT0064 0.10 -1.1 59,624 - BT0064 0.11 +1.4 59,624 - BT0064 0.11 -0.3 59,624 - BT0064 0.11 -1.6 59,624 - BT0064 0.11 -2.1 59,624 - BT0064 0.11 -1.6 59,624 - BT0064 0.11 -2.5 59,629 + BT0064 0.12 -1.7 59,668 + BT0064 0.13 -0.1 59,669 - BT0064 0.13 -1.1 59,767 + BT0064 0.18 +0.3
Or see this region's nucleotide sequence