Experiment: MoLS4 with L-Arginine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prfC and DVU3117 are separated by 282 nucleotides DVU3117 and DVU3118 are separated by 127 nucleotides DVU3118 and DVU3119 are separated by 180 nucleotides
DVU3116: prfC - peptide chain release factor 3 (TIGR), at 3,264,560 to 3,266,149
prfC
DVU3117: DVU3117 - hypothetical protein (TIGR), at 3,266,432 to 3,267,016
DVU3117
DVU3118: DVU3118 - conserved hypothetical protein (TIGR), at 3,267,144 to 3,267,449
DVU3118
DVU3119: DVU3119 - AMP-binding enzyme family protein (TIGR), at 3,267,630 to 3,269,324
DVU3119
Position (kb)
3266
3267
3268 Strain fitness (log2 ratio)
-2
-1
0
1 at 3265.469 kb on + strand, within prfC at 3265.483 kb on - strand, within prfC at 3265.569 kb on - strand, within prfC at 3265.570 kb on - strand, within prfC at 3265.575 kb on - strand, within prfC at 3265.779 kb on + strand, within prfC at 3265.799 kb on + strand, within prfC at 3265.857 kb on - strand, within prfC at 3265.881 kb on + strand, within prfC at 3265.929 kb on + strand, within prfC at 3265.989 kb on + strand, within prfC at 3266.243 kb on + strand at 3266.310 kb on + strand at 3266.313 kb on - strand at 3266.326 kb on - strand at 3266.328 kb on + strand at 3266.329 kb on + strand at 3266.339 kb on + strand at 3266.407 kb on - strand at 3266.437 kb on + strand at 3266.445 kb on - strand at 3266.616 kb on + strand, within DVU3117 at 3266.858 kb on + strand, within DVU3117 at 3266.866 kb on - strand, within DVU3117 at 3266.992 kb on + strand at 3266.992 kb on + strand at 3267.000 kb on - strand at 3267.005 kb on + strand at 3267.013 kb on - strand at 3267.080 kb on - strand at 3267.096 kb on + strand at 3267.137 kb on - strand at 3267.204 kb on + strand, within DVU3118 at 3267.212 kb on - strand, within DVU3118 at 3267.212 kb on - strand, within DVU3118 at 3267.284 kb on + strand, within DVU3118 at 3267.317 kb on + strand, within DVU3118 at 3267.325 kb on - strand, within DVU3118 at 3267.355 kb on - strand, within DVU3118 at 3267.458 kb on - strand at 3267.480 kb on + strand at 3267.488 kb on - strand at 3267.515 kb on + strand at 3267.563 kb on - strand at 3267.572 kb on + strand at 3267.594 kb on + strand at 3267.597 kb on - strand at 3267.602 kb on - strand at 3267.611 kb on - strand at 3267.645 kb on - strand at 3267.676 kb on + strand at 3267.686 kb on - strand at 3267.691 kb on - strand at 3267.744 kb on + strand at 3267.830 kb on - strand, within DVU3119 at 3267.894 kb on - strand, within DVU3119 at 3267.918 kb on + strand, within DVU3119 at 3267.926 kb on - strand, within DVU3119 at 3267.945 kb on - strand, within DVU3119 at 3267.990 kb on - strand, within DVU3119 at 3267.992 kb on + strand, within DVU3119 at 3268.001 kb on - strand, within DVU3119 at 3268.014 kb on + strand, within DVU3119
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Arginine as nutrient 10mM remove 3,265,469 + prfC DVU3116 0.57 -0.4 3,265,483 - prfC DVU3116 0.58 -0.5 3,265,569 - prfC DVU3116 0.63 -0.6 3,265,570 - prfC DVU3116 0.64 -0.0 3,265,575 - prfC DVU3116 0.64 -0.1 3,265,779 + prfC DVU3116 0.77 -0.6 3,265,799 + prfC DVU3116 0.78 -1.0 3,265,857 - prfC DVU3116 0.82 -1.2 3,265,881 + prfC DVU3116 0.83 -0.2 3,265,929 + prfC DVU3116 0.86 -0.6 3,265,989 + prfC DVU3116 0.90 -0.5 3,266,243 + +0.2 3,266,310 + +0.5 3,266,313 - -0.5 3,266,326 - -0.3 3,266,328 + +0.5 3,266,329 + +0.0 3,266,339 + +0.0 3,266,407 - +0.0 3,266,437 + -0.5 3,266,445 - +0.3 3,266,616 + DVU3117 0.31 +1.0 3,266,858 + DVU3117 0.73 +0.1 3,266,866 - DVU3117 0.74 +0.2 3,266,992 + +1.4 3,266,992 + +0.0 3,267,000 - -0.3 3,267,005 + -1.3 3,267,013 - +0.2 3,267,080 - -0.6 3,267,096 + -0.1 3,267,137 - -0.5 3,267,204 + DVU3118 0.20 +1.3 3,267,212 - DVU3118 0.22 +0.4 3,267,212 - DVU3118 0.22 +0.6 3,267,284 + DVU3118 0.46 +0.1 3,267,317 + DVU3118 0.57 -0.4 3,267,325 - DVU3118 0.59 -1.2 3,267,355 - DVU3118 0.69 -0.3 3,267,458 - -0.9 3,267,480 + -0.5 3,267,488 - -0.3 3,267,515 + -0.5 3,267,563 - +0.4 3,267,572 + -0.7 3,267,594 + -0.6 3,267,597 - -0.3 3,267,602 - -2.0 3,267,611 - +1.1 3,267,645 - +0.3 3,267,676 + -0.3 3,267,686 - +1.4 3,267,691 - -0.3 3,267,744 + +0.1 3,267,830 - DVU3119 0.12 -1.4 3,267,894 - DVU3119 0.16 +0.7 3,267,918 + DVU3119 0.17 -1.0 3,267,926 - DVU3119 0.17 +0.4 3,267,945 - DVU3119 0.19 -0.3 3,267,990 - DVU3119 0.21 -0.6 3,267,992 + DVU3119 0.21 -0.4 3,268,001 - DVU3119 0.22 +0.1 3,268,014 + DVU3119 0.23 -1.6
Or see this region's nucleotide sequence