Experiment: BHIS with Sulfamethizole 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2417 and BT2418 are separated by 124 nucleotides BT2418 and tRNA-Met are separated by 224 nucleotides tRNA-Met and BT2419 are separated by 166 nucleotides BT2419 and BT2420 are separated by 185 nucleotides
BT2417: BT2417 - conserved hypothetical protein (NCBI ptt file), at 3,018,122 to 3,019,972
BT2417
BT2418: BT2418 - hypothetical protein (NCBI ptt file), at 3,020,097 to 3,020,483
BT2418
BT_t37: tRNA-Met - tRNA-Met (RefSeq), at 3,020,708 to 3,020,781
tRNA-Met
BT2419: BT2419 - putative GMP synthase [glutamine-hydrolyzing] (NCBI ptt file), at 3,020,948 to 3,022,255
BT2419
BT2420: BT2420 - two-component system histidine kinase, FrrD (NCBI ptt file), at 3,022,441 to 3,023,889
BT2420
Position (kb)
3020
3021
3022
3023 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3020.041 kb on - strand at 3020.041 kb on - strand at 3020.098 kb on + strand at 3020.194 kb on - strand, within BT2418 at 3020.196 kb on + strand, within BT2418 at 3020.200 kb on + strand, within BT2418 at 3020.201 kb on - strand, within BT2418 at 3020.201 kb on - strand, within BT2418 at 3020.220 kb on - strand, within BT2418 at 3020.221 kb on + strand, within BT2418 at 3020.221 kb on + strand, within BT2418 at 3020.221 kb on + strand, within BT2418 at 3020.349 kb on + strand, within BT2418 at 3020.357 kb on + strand, within BT2418 at 3020.358 kb on - strand, within BT2418 at 3020.371 kb on - strand, within BT2418 at 3020.412 kb on - strand, within BT2418 at 3020.415 kb on - strand, within BT2418 at 3020.415 kb on - strand, within BT2418 at 3020.435 kb on + strand, within BT2418 at 3020.455 kb on + strand at 3020.503 kb on + strand at 3020.544 kb on + strand at 3020.787 kb on + strand at 3020.793 kb on + strand at 3020.794 kb on - strand at 3020.838 kb on + strand at 3020.838 kb on + strand at 3020.838 kb on + strand at 3020.865 kb on + strand at 3020.866 kb on - strand at 3020.907 kb on - strand at 3020.929 kb on + strand at 3020.930 kb on - strand at 3020.930 kb on - strand at 3020.942 kb on - strand at 3020.944 kb on - strand at 3020.950 kb on - strand at 3020.987 kb on - strand at 3020.987 kb on - strand at 3020.991 kb on + strand at 3020.991 kb on + strand at 3020.991 kb on + strand at 3020.992 kb on - strand at 3021.029 kb on - strand at 3021.074 kb on - strand at 3021.077 kb on - strand at 3021.093 kb on - strand, within BT2419 at 3021.254 kb on + strand, within BT2419 at 3021.296 kb on + strand, within BT2419 at 3021.297 kb on - strand, within BT2419 at 3021.342 kb on + strand, within BT2419 at 3021.342 kb on + strand, within BT2419 at 3021.443 kb on + strand, within BT2419 at 3021.449 kb on - strand, within BT2419 at 3021.449 kb on - strand, within BT2419 at 3021.505 kb on - strand, within BT2419 at 3021.545 kb on - strand, within BT2419 at 3021.648 kb on - strand, within BT2419 at 3021.653 kb on + strand, within BT2419 at 3021.654 kb on - strand, within BT2419 at 3021.654 kb on - strand, within BT2419 at 3021.780 kb on - strand, within BT2419 at 3021.827 kb on + strand, within BT2419 at 3021.923 kb on + strand, within BT2419 at 3021.923 kb on + strand, within BT2419 at 3021.924 kb on - strand, within BT2419 at 3021.935 kb on + strand, within BT2419 at 3021.938 kb on - strand, within BT2419 at 3021.946 kb on + strand, within BT2419 at 3021.958 kb on + strand, within BT2419 at 3021.968 kb on - strand, within BT2419 at 3022.037 kb on - strand, within BT2419 at 3022.039 kb on - strand, within BT2419 at 3022.099 kb on + strand, within BT2419 at 3022.102 kb on + strand, within BT2419 at 3022.103 kb on - strand, within BT2419 at 3022.103 kb on - strand, within BT2419 at 3022.105 kb on + strand, within BT2419 at 3022.105 kb on + strand, within BT2419 at 3022.170 kb on + strand at 3022.170 kb on + strand at 3022.235 kb on - strand at 3022.272 kb on + strand at 3022.273 kb on - strand at 3022.278 kb on - strand at 3022.281 kb on - strand at 3022.281 kb on - strand at 3022.283 kb on - strand at 3022.312 kb on - strand at 3022.599 kb on + strand, within BT2420 at 3022.660 kb on - strand, within BT2420 at 3022.750 kb on + strand, within BT2420 at 3022.801 kb on + strand, within BT2420 at 3022.848 kb on + strand, within BT2420 at 3022.856 kb on + strand, within BT2420 at 3022.856 kb on + strand, within BT2420 at 3022.857 kb on - strand, within BT2420 at 3022.857 kb on - strand, within BT2420 at 3022.862 kb on - strand, within BT2420 at 3022.883 kb on - strand, within BT2420 at 3022.894 kb on + strand, within BT2420 at 3022.895 kb on - strand, within BT2420 at 3022.927 kb on - strand, within BT2420 at 3023.035 kb on + strand, within BT2420 at 3023.098 kb on - strand, within BT2420 at 3023.104 kb on - strand, within BT2420 at 3023.140 kb on - strand, within BT2420 at 3023.140 kb on - strand, within BT2420 at 3023.165 kb on - strand, within BT2420 at 3023.200 kb on + strand, within BT2420 at 3023.213 kb on + strand, within BT2420 at 3023.213 kb on + strand, within BT2420 at 3023.250 kb on - strand, within BT2420 at 3023.251 kb on + strand, within BT2420 at 3023.251 kb on + strand, within BT2420
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfamethizole 0.1 mM remove 3,020,041 - -0.0 3,020,041 - -1.6 3,020,098 + -1.6 3,020,194 - BT2418 0.25 +0.6 3,020,196 + BT2418 0.26 -1.6 3,020,200 + BT2418 0.27 -1.0 3,020,201 - BT2418 0.27 -3.9 3,020,201 - BT2418 0.27 -1.0 3,020,220 - BT2418 0.32 -0.0 3,020,221 + BT2418 0.32 -2.0 3,020,221 + BT2418 0.32 -2.4 3,020,221 + BT2418 0.32 -1.6 3,020,349 + BT2418 0.65 -1.8 3,020,357 + BT2418 0.67 -0.0 3,020,358 - BT2418 0.67 -3.5 3,020,371 - BT2418 0.71 -1.0 3,020,412 - BT2418 0.81 -3.6 3,020,415 - BT2418 0.82 -1.6 3,020,415 - BT2418 0.82 -1.6 3,020,435 + BT2418 0.87 -3.9 3,020,455 + -0.0 3,020,503 + -1.0 3,020,544 + -2.5 3,020,787 + -1.0 3,020,793 + -0.6 3,020,794 - -2.5 3,020,838 + -1.4 3,020,838 + -0.0 3,020,838 + +2.8 3,020,865 + -1.2 3,020,866 - -2.0 3,020,907 - -1.6 3,020,929 + -1.5 3,020,930 - -0.0 3,020,930 - +1.0 3,020,942 - -1.4 3,020,944 - -1.4 3,020,950 - -0.0 3,020,987 - -0.4 3,020,987 - -3.3 3,020,991 + -0.8 3,020,991 + -0.7 3,020,991 + -3.7 3,020,992 - -0.0 3,021,029 - -1.5 3,021,074 - -2.0 3,021,077 - -2.9 3,021,093 - BT2419 0.11 -0.4 3,021,254 + BT2419 0.23 -0.6 3,021,296 + BT2419 0.27 -0.7 3,021,297 - BT2419 0.27 -1.6 3,021,342 + BT2419 0.30 -1.0 3,021,342 + BT2419 0.30 -1.6 3,021,443 + BT2419 0.38 -1.6 3,021,449 - BT2419 0.38 +1.0 3,021,449 - BT2419 0.38 +0.2 3,021,505 - BT2419 0.43 +1.0 3,021,545 - BT2419 0.46 +2.0 3,021,648 - BT2419 0.54 -0.5 3,021,653 + BT2419 0.54 -0.6 3,021,654 - BT2419 0.54 -1.1 3,021,654 - BT2419 0.54 -1.6 3,021,780 - BT2419 0.64 -0.0 3,021,827 + BT2419 0.67 -4.1 3,021,923 + BT2419 0.75 -0.0 3,021,923 + BT2419 0.75 -0.6 3,021,924 - BT2419 0.75 -2.5 3,021,935 + BT2419 0.75 +0.6 3,021,938 - BT2419 0.76 -2.2 3,021,946 + BT2419 0.76 -0.6 3,021,958 + BT2419 0.77 -1.9 3,021,968 - BT2419 0.78 -1.0 3,022,037 - BT2419 0.83 -1.8 3,022,039 - BT2419 0.83 -1.0 3,022,099 + BT2419 0.88 +0.4 3,022,102 + BT2419 0.88 -1.0 3,022,103 - BT2419 0.88 -1.0 3,022,103 - BT2419 0.88 -1.6 3,022,105 + BT2419 0.88 -1.0 3,022,105 + BT2419 0.88 -1.6 3,022,170 + -1.0 3,022,170 + -1.5 3,022,235 - -0.0 3,022,272 + -0.9 3,022,273 - -1.0 3,022,278 - -0.2 3,022,281 - -1.6 3,022,281 - -0.0 3,022,283 - -1.2 3,022,312 - -0.7 3,022,599 + BT2420 0.11 -1.0 3,022,660 - BT2420 0.15 -2.5 3,022,750 + BT2420 0.21 -2.4 3,022,801 + BT2420 0.25 +0.4 3,022,848 + BT2420 0.28 -1.6 3,022,856 + BT2420 0.29 -2.0 3,022,856 + BT2420 0.29 -0.0 3,022,857 - BT2420 0.29 -1.8 3,022,857 - BT2420 0.29 +1.6 3,022,862 - BT2420 0.29 -1.6 3,022,883 - BT2420 0.31 -2.4 3,022,894 + BT2420 0.31 -2.0 3,022,895 - BT2420 0.31 -1.8 3,022,927 - BT2420 0.34 -1.3 3,023,035 + BT2420 0.41 -0.6 3,023,098 - BT2420 0.45 +0.6 3,023,104 - BT2420 0.46 +1.0 3,023,140 - BT2420 0.48 -0.8 3,023,140 - BT2420 0.48 -1.7 3,023,165 - BT2420 0.50 -1.7 3,023,200 + BT2420 0.52 -1.7 3,023,213 + BT2420 0.53 -0.0 3,023,213 + BT2420 0.53 -1.5 3,023,250 - BT2420 0.56 -0.0 3,023,251 + BT2420 0.56 -3.2 3,023,251 + BT2420 0.56 -0.4
Or see this region's nucleotide sequence