Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4615 and Psyr_4616 are separated by 425 nucleotides Psyr_4616 and Psyr_4617 overlap by 1 nucleotides Psyr_4617 and Psyr_4618 are separated by 177 nucleotides
Psyr_4615: Psyr_4615 - Spermidine/putrescine ABC transporter ATP-binding subunit, at 5,472,997 to 5,474,121
_4615
Psyr_4616: Psyr_4616 - Integrase, catalytic region, at 5,474,547 to 5,475,383
_4616
Psyr_4617: Psyr_4617 - ISPsy9, transposase OrfA, at 5,475,383 to 5,475,922
_4617
Psyr_4618: Psyr_4618 - regulatory protein, LuxR:Response regulator receiver, at 5,476,100 to 5,476,729
_4618
Position (kb) 5474
5475
5476 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5473.713 kb on + strand, within Psyr_4615 at 5473.835 kb on + strand, within Psyr_4615 at 5473.835 kb on + strand, within Psyr_4615 at 5473.835 kb on + strand, within Psyr_4615 at 5473.836 kb on - strand, within Psyr_4615 at 5473.836 kb on - strand, within Psyr_4615 at 5473.836 kb on - strand, within Psyr_4615 at 5473.836 kb on - strand, within Psyr_4615 at 5473.866 kb on + strand, within Psyr_4615 at 5474.051 kb on + strand at 5474.051 kb on + strand at 5474.052 kb on - strand at 5474.052 kb on - strand at 5474.159 kb on + strand at 5474.159 kb on + strand at 5474.160 kb on - strand at 5474.263 kb on + strand at 5474.264 kb on - strand at 5474.354 kb on + strand at 5474.354 kb on + strand at 5474.477 kb on + strand at 5474.477 kb on + strand at 5474.478 kb on - strand at 5474.478 kb on - strand at 5474.478 kb on - strand at 5474.478 kb on - strand at 5474.478 kb on - strand at 5474.478 kb on - strand at 5474.478 kb on - strand at 5476.055 kb on + strand at 5476.055 kb on + strand at 5476.055 kb on + strand at 5476.055 kb on + strand at 5476.055 kb on + strand at 5476.055 kb on + strand at 5476.126 kb on - strand at 5476.126 kb on - strand at 5476.126 kb on - strand at 5476.359 kb on + strand, within Psyr_4618 at 5476.360 kb on - strand, within Psyr_4618 at 5476.360 kb on - strand, within Psyr_4618
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 5,473,713 + Psyr_4615 0.64 +0.5 5,473,835 + Psyr_4615 0.74 -1.9 5,473,835 + Psyr_4615 0.74 +0.2 5,473,835 + Psyr_4615 0.74 -0.0 5,473,836 - Psyr_4615 0.75 +0.8 5,473,836 - Psyr_4615 0.75 -0.1 5,473,836 - Psyr_4615 0.75 +0.4 5,473,836 - Psyr_4615 0.75 +1.2 5,473,866 + Psyr_4615 0.77 -0.4 5,474,051 + +2.2 5,474,051 + -1.6 5,474,052 - +0.4 5,474,052 - +1.8 5,474,159 + -0.5 5,474,159 + +1.0 5,474,160 - -0.3 5,474,263 + +0.0 5,474,264 - +0.2 5,474,354 + +0.3 5,474,354 + +0.2 5,474,477 + -0.2 5,474,477 + -0.0 5,474,478 - -1.1 5,474,478 - -1.5 5,474,478 - -1.7 5,474,478 - +1.2 5,474,478 - +0.1 5,474,478 - -0.8 5,474,478 - +2.8 5,476,055 + -1.4 5,476,055 + -0.5 5,476,055 + +4.1 5,476,055 + +0.2 5,476,055 + +1.4 5,476,055 + -0.7 5,476,126 - +0.6 5,476,126 - +1.2 5,476,126 - -1.8 5,476,359 + Psyr_4618 0.41 -1.5 5,476,360 - Psyr_4618 0.41 +1.2 5,476,360 - Psyr_4618 0.41 +0.0
Or see this region's nucleotide sequence