Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3779 and Psyr_3780 are separated by 24 nucleotides Psyr_3780 and Psyr_3781 are separated by 50 nucleotides Psyr_3781 and Psyr_3782 are separated by 223 nucleotides Psyr_3782 and Psyr_3783 are separated by 212 nucleotides
Psyr_3779: Psyr_3779 - GCN5-related N-acetyltransferase, at 4,497,536 to 4,497,982
_3779
Psyr_3780: Psyr_3780 - Formylmethionine deformylase, at 4,498,007 to 4,498,546
_3780
Psyr_3781: Psyr_3781 - Ribonuclease BN, at 4,498,597 to 4,499,562
_3781
Psyr_3782: Psyr_3782 - CsbD-like protein, at 4,499,786 to 4,499,977
_3782
Psyr_3783: Psyr_3783 - conserved hypothetical protein, at 4,500,190 to 4,500,570
_3783
Position (kb)
4498
4499
4500 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4497.632 kb on + strand, within Psyr_3779 at 4497.633 kb on - strand, within Psyr_3779 at 4497.873 kb on - strand, within Psyr_3779 at 4497.873 kb on - strand, within Psyr_3779 at 4497.873 kb on - strand, within Psyr_3779 at 4498.788 kb on + strand, within Psyr_3781 at 4498.790 kb on + strand, within Psyr_3781 at 4498.790 kb on + strand, within Psyr_3781 at 4498.790 kb on + strand, within Psyr_3781 at 4498.791 kb on - strand, within Psyr_3781 at 4498.791 kb on - strand, within Psyr_3781 at 4498.791 kb on - strand, within Psyr_3781 at 4498.791 kb on - strand, within Psyr_3781 at 4498.796 kb on + strand, within Psyr_3781 at 4498.796 kb on + strand, within Psyr_3781 at 4498.796 kb on + strand, within Psyr_3781 at 4498.796 kb on + strand, within Psyr_3781 at 4498.796 kb on + strand, within Psyr_3781 at 4498.796 kb on + strand, within Psyr_3781 at 4498.796 kb on + strand, within Psyr_3781 at 4498.797 kb on - strand, within Psyr_3781 at 4498.797 kb on - strand, within Psyr_3781 at 4498.797 kb on - strand, within Psyr_3781 at 4498.797 kb on - strand, within Psyr_3781 at 4498.833 kb on - strand, within Psyr_3781 at 4498.833 kb on - strand, within Psyr_3781 at 4498.844 kb on + strand, within Psyr_3781 at 4498.845 kb on - strand, within Psyr_3781 at 4498.845 kb on - strand, within Psyr_3781 at 4498.845 kb on - strand, within Psyr_3781 at 4498.866 kb on - strand, within Psyr_3781 at 4498.964 kb on + strand, within Psyr_3781 at 4498.964 kb on + strand, within Psyr_3781 at 4498.964 kb on + strand, within Psyr_3781 at 4498.964 kb on + strand, within Psyr_3781 at 4498.964 kb on + strand, within Psyr_3781 at 4498.965 kb on - strand, within Psyr_3781 at 4498.965 kb on - strand, within Psyr_3781 at 4498.965 kb on - strand, within Psyr_3781 at 4498.965 kb on - strand, within Psyr_3781 at 4498.965 kb on - strand, within Psyr_3781 at 4498.965 kb on - strand, within Psyr_3781 at 4498.967 kb on + strand, within Psyr_3781 at 4498.967 kb on + strand, within Psyr_3781 at 4498.967 kb on + strand, within Psyr_3781 at 4498.967 kb on + strand, within Psyr_3781 at 4498.967 kb on + strand, within Psyr_3781 at 4498.967 kb on + strand, within Psyr_3781 at 4498.968 kb on - strand, within Psyr_3781 at 4498.968 kb on - strand, within Psyr_3781 at 4498.968 kb on - strand, within Psyr_3781 at 4498.968 kb on - strand, within Psyr_3781 at 4498.998 kb on + strand, within Psyr_3781 at 4498.999 kb on - strand, within Psyr_3781 at 4499.120 kb on - strand, within Psyr_3781 at 4499.138 kb on - strand, within Psyr_3781 at 4499.144 kb on + strand, within Psyr_3781 at 4499.144 kb on + strand, within Psyr_3781 at 4499.144 kb on + strand, within Psyr_3781 at 4499.145 kb on - strand, within Psyr_3781 at 4499.175 kb on + strand, within Psyr_3781 at 4499.176 kb on - strand, within Psyr_3781 at 4499.198 kb on + strand, within Psyr_3781 at 4499.198 kb on + strand, within Psyr_3781 at 4499.199 kb on - strand, within Psyr_3781 at 4499.199 kb on - strand, within Psyr_3781 at 4499.199 kb on - strand, within Psyr_3781 at 4499.456 kb on + strand, within Psyr_3781 at 4499.474 kb on + strand at 4499.474 kb on + strand at 4499.474 kb on + strand at 4499.474 kb on + strand at 4499.474 kb on + strand at 4499.474 kb on + strand at 4499.475 kb on - strand at 4499.475 kb on - strand at 4499.475 kb on - strand at 4499.475 kb on - strand at 4499.475 kb on - strand at 4499.475 kb on - strand at 4499.475 kb on - strand at 4499.475 kb on - strand at 4499.475 kb on - strand at 4499.596 kb on + strand at 4499.596 kb on + strand at 4499.596 kb on + strand at 4499.597 kb on - strand at 4499.597 kb on - strand at 4499.597 kb on - strand at 4499.597 kb on - strand at 4499.597 kb on - strand at 4499.620 kb on + strand at 4499.620 kb on + strand at 4499.620 kb on + strand at 4499.620 kb on + strand at 4499.620 kb on + strand at 4499.620 kb on + strand at 4499.620 kb on + strand at 4499.620 kb on + strand at 4499.620 kb on + strand at 4499.620 kb on + strand at 4499.620 kb on + strand at 4499.621 kb on - strand at 4499.621 kb on - strand at 4499.621 kb on - strand at 4499.621 kb on - strand at 4499.621 kb on - strand at 4499.621 kb on - strand at 4499.621 kb on - strand at 4499.718 kb on - strand at 4499.747 kb on + strand at 4499.831 kb on + strand, within Psyr_3782 at 4499.831 kb on + strand, within Psyr_3782 at 4499.864 kb on + strand, within Psyr_3782 at 4499.864 kb on + strand, within Psyr_3782 at 4499.865 kb on - strand, within Psyr_3782 at 4499.865 kb on - strand, within Psyr_3782 at 4499.948 kb on - strand, within Psyr_3782 at 4500.067 kb on + strand at 4500.067 kb on + strand at 4500.336 kb on - strand, within Psyr_3783 at 4500.343 kb on + strand, within Psyr_3783 at 4500.344 kb on - strand, within Psyr_3783 at 4500.344 kb on - strand, within Psyr_3783 at 4500.413 kb on + strand, within Psyr_3783 at 4500.414 kb on - strand, within Psyr_3783
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 4,497,632 + Psyr_3779 0.21 +0.5 4,497,633 - Psyr_3779 0.22 +1.0 4,497,873 - Psyr_3779 0.75 +0.4 4,497,873 - Psyr_3779 0.75 -1.0 4,497,873 - Psyr_3779 0.75 -0.6 4,498,788 + Psyr_3781 0.20 +0.4 4,498,790 + Psyr_3781 0.20 +0.3 4,498,790 + Psyr_3781 0.20 -0.2 4,498,790 + Psyr_3781 0.20 -0.8 4,498,791 - Psyr_3781 0.20 -0.5 4,498,791 - Psyr_3781 0.20 +0.5 4,498,791 - Psyr_3781 0.20 +0.9 4,498,791 - Psyr_3781 0.20 +0.6 4,498,796 + Psyr_3781 0.21 -1.0 4,498,796 + Psyr_3781 0.21 +3.5 4,498,796 + Psyr_3781 0.21 -0.0 4,498,796 + Psyr_3781 0.21 -1.2 4,498,796 + Psyr_3781 0.21 -2.0 4,498,796 + Psyr_3781 0.21 -2.0 4,498,796 + Psyr_3781 0.21 -0.8 4,498,797 - Psyr_3781 0.21 +1.0 4,498,797 - Psyr_3781 0.21 -1.5 4,498,797 - Psyr_3781 0.21 +1.1 4,498,797 - Psyr_3781 0.21 -0.6 4,498,833 - Psyr_3781 0.24 -0.0 4,498,833 - Psyr_3781 0.24 -1.1 4,498,844 + Psyr_3781 0.26 -1.6 4,498,845 - Psyr_3781 0.26 -1.6 4,498,845 - Psyr_3781 0.26 -0.5 4,498,845 - Psyr_3781 0.26 +0.1 4,498,866 - Psyr_3781 0.28 +0.8 4,498,964 + Psyr_3781 0.38 +0.4 4,498,964 + Psyr_3781 0.38 +3.5 4,498,964 + Psyr_3781 0.38 +0.8 4,498,964 + Psyr_3781 0.38 -0.7 4,498,964 + Psyr_3781 0.38 -0.5 4,498,965 - Psyr_3781 0.38 -1.4 4,498,965 - Psyr_3781 0.38 -0.8 4,498,965 - Psyr_3781 0.38 -1.3 4,498,965 - Psyr_3781 0.38 +0.7 4,498,965 - Psyr_3781 0.38 +1.7 4,498,965 - Psyr_3781 0.38 -0.6 4,498,967 + Psyr_3781 0.38 +1.5 4,498,967 + Psyr_3781 0.38 -0.7 4,498,967 + Psyr_3781 0.38 -0.9 4,498,967 + Psyr_3781 0.38 +1.0 4,498,967 + Psyr_3781 0.38 +0.2 4,498,967 + Psyr_3781 0.38 -0.6 4,498,968 - Psyr_3781 0.38 +2.9 4,498,968 - Psyr_3781 0.38 +0.5 4,498,968 - Psyr_3781 0.38 -2.0 4,498,968 - Psyr_3781 0.38 +1.5 4,498,998 + Psyr_3781 0.42 +0.1 4,498,999 - Psyr_3781 0.42 +0.2 4,499,120 - Psyr_3781 0.54 -0.8 4,499,138 - Psyr_3781 0.56 -0.8 4,499,144 + Psyr_3781 0.57 -1.0 4,499,144 + Psyr_3781 0.57 -2.8 4,499,144 + Psyr_3781 0.57 +3.1 4,499,145 - Psyr_3781 0.57 +0.7 4,499,175 + Psyr_3781 0.60 -2.8 4,499,176 - Psyr_3781 0.60 -1.3 4,499,198 + Psyr_3781 0.62 -0.5 4,499,198 + Psyr_3781 0.62 -0.5 4,499,199 - Psyr_3781 0.62 +1.5 4,499,199 - Psyr_3781 0.62 -0.8 4,499,199 - Psyr_3781 0.62 -0.6 4,499,456 + Psyr_3781 0.89 -1.8 4,499,474 + -0.8 4,499,474 + +0.2 4,499,474 + -0.5 4,499,474 + +0.2 4,499,474 + +1.5 4,499,474 + -0.0 4,499,475 - -0.5 4,499,475 - +0.5 4,499,475 - -1.7 4,499,475 - +2.4 4,499,475 - -0.5 4,499,475 - +0.8 4,499,475 - -0.5 4,499,475 - +0.5 4,499,475 - +0.4 4,499,596 + -0.2 4,499,596 + -0.5 4,499,596 + -0.0 4,499,597 - -0.0 4,499,597 - +0.4 4,499,597 - +0.4 4,499,597 - -0.8 4,499,597 - -0.0 4,499,620 + +0.7 4,499,620 + -0.8 4,499,620 + -0.0 4,499,620 + -0.4 4,499,620 + +0.9 4,499,620 + +0.5 4,499,620 + -0.6 4,499,620 + -0.7 4,499,620 + +0.5 4,499,620 + -1.3 4,499,620 + -0.0 4,499,621 - +4.4 4,499,621 - -0.5 4,499,621 - -0.5 4,499,621 - +1.5 4,499,621 - -0.5 4,499,621 - -0.4 4,499,621 - +3.1 4,499,718 - +0.0 4,499,747 + +2.1 4,499,831 + Psyr_3782 0.23 +1.5 4,499,831 + Psyr_3782 0.23 -0.7 4,499,864 + Psyr_3782 0.41 -0.6 4,499,864 + Psyr_3782 0.41 +0.5 4,499,865 - Psyr_3782 0.41 -0.6 4,499,865 - Psyr_3782 0.41 -0.5 4,499,948 - Psyr_3782 0.84 -0.9 4,500,067 + +0.0 4,500,067 + -0.1 4,500,336 - Psyr_3783 0.38 +0.1 4,500,343 + Psyr_3783 0.40 +1.8 4,500,344 - Psyr_3783 0.40 -0.7 4,500,344 - Psyr_3783 0.40 -0.2 4,500,413 + Psyr_3783 0.59 -0.0 4,500,414 - Psyr_3783 0.59 +1.3
Or see this region's nucleotide sequence