Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3506 and Psyr_3507 are separated by 10 nucleotides Psyr_3507 and Psyr_3508 overlap by 4 nucleotides
Psyr_3506: Psyr_3506 - Sulfatase, at 4,180,950 to 4,182,983
_3506
Psyr_3507: Psyr_3507 - membrane protein, putative, at 4,182,994 to 4,183,614
_3507
Psyr_3508: Psyr_3508 - Glycosyl hydrolase, BNR repeat protein, at 4,183,611 to 4,184,909
_3508
Position (kb)
4182
4183
4184 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4182.200 kb on - strand at 4182.218 kb on - strand, within Psyr_3506 at 4182.502 kb on - strand, within Psyr_3506 at 4182.560 kb on - strand, within Psyr_3506 at 4182.560 kb on - strand, within Psyr_3506 at 4182.641 kb on - strand, within Psyr_3506 at 4182.646 kb on + strand, within Psyr_3506 at 4182.646 kb on + strand, within Psyr_3506 at 4182.667 kb on + strand, within Psyr_3506 at 4182.667 kb on + strand, within Psyr_3506 at 4182.667 kb on + strand, within Psyr_3506 at 4182.667 kb on + strand, within Psyr_3506 at 4182.667 kb on + strand, within Psyr_3506 at 4182.667 kb on + strand, within Psyr_3506 at 4182.667 kb on + strand, within Psyr_3506 at 4182.667 kb on + strand, within Psyr_3506 at 4182.667 kb on + strand, within Psyr_3506 at 4182.668 kb on - strand, within Psyr_3506 at 4182.668 kb on - strand, within Psyr_3506 at 4182.668 kb on - strand, within Psyr_3506 at 4182.668 kb on - strand, within Psyr_3506 at 4182.668 kb on - strand, within Psyr_3506 at 4182.668 kb on - strand, within Psyr_3506 at 4182.668 kb on - strand, within Psyr_3506 at 4182.668 kb on - strand, within Psyr_3506 at 4182.668 kb on - strand, within Psyr_3506 at 4182.668 kb on - strand, within Psyr_3506 at 4182.889 kb on + strand at 4182.890 kb on - strand at 4182.996 kb on - strand at 4183.091 kb on + strand, within Psyr_3507 at 4183.091 kb on + strand, within Psyr_3507 at 4183.091 kb on + strand, within Psyr_3507 at 4183.207 kb on - strand, within Psyr_3507 at 4183.286 kb on + strand, within Psyr_3507 at 4183.286 kb on + strand, within Psyr_3507 at 4183.286 kb on + strand, within Psyr_3507 at 4183.460 kb on + strand, within Psyr_3507 at 4183.461 kb on - strand, within Psyr_3507 at 4183.614 kb on + strand at 4183.614 kb on + strand at 4183.670 kb on + strand at 4183.681 kb on + strand at 4183.682 kb on - strand at 4183.682 kb on - strand at 4183.694 kb on - strand at 4183.694 kb on - strand at 4183.720 kb on + strand at 4183.727 kb on - strand at 4183.727 kb on - strand at 4183.855 kb on + strand, within Psyr_3508 at 4183.855 kb on + strand, within Psyr_3508 at 4184.248 kb on + strand, within Psyr_3508 at 4184.255 kb on - strand, within Psyr_3508 at 4184.255 kb on - strand, within Psyr_3508 at 4184.255 kb on - strand, within Psyr_3508 at 4184.284 kb on + strand, within Psyr_3508 at 4184.334 kb on + strand, within Psyr_3508 at 4184.343 kb on - strand, within Psyr_3508 at 4184.464 kb on + strand, within Psyr_3508 at 4184.464 kb on + strand, within Psyr_3508 at 4184.464 kb on + strand, within Psyr_3508 at 4184.465 kb on - strand, within Psyr_3508 at 4184.465 kb on - strand, within Psyr_3508 at 4184.527 kb on + strand, within Psyr_3508 at 4184.527 kb on + strand, within Psyr_3508 at 4184.527 kb on + strand, within Psyr_3508 at 4184.527 kb on + strand, within Psyr_3508 at 4184.527 kb on + strand, within Psyr_3508 at 4184.528 kb on - strand, within Psyr_3508 at 4184.528 kb on - strand, within Psyr_3508 at 4184.528 kb on - strand, within Psyr_3508
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 4,182,200 - -1.2 4,182,218 - Psyr_3506 0.62 +0.7 4,182,502 - Psyr_3506 0.76 -0.8 4,182,560 - Psyr_3506 0.79 -1.6 4,182,560 - Psyr_3506 0.79 +1.6 4,182,641 - Psyr_3506 0.83 -0.8 4,182,646 + Psyr_3506 0.83 +0.7 4,182,646 + Psyr_3506 0.83 -1.0 4,182,667 + Psyr_3506 0.84 -3.0 4,182,667 + Psyr_3506 0.84 -0.1 4,182,667 + Psyr_3506 0.84 +2.4 4,182,667 + Psyr_3506 0.84 -0.3 4,182,667 + Psyr_3506 0.84 -0.4 4,182,667 + Psyr_3506 0.84 -0.6 4,182,667 + Psyr_3506 0.84 +0.2 4,182,667 + Psyr_3506 0.84 -0.6 4,182,667 + Psyr_3506 0.84 +2.4 4,182,668 - Psyr_3506 0.84 +0.9 4,182,668 - Psyr_3506 0.84 -1.0 4,182,668 - Psyr_3506 0.84 +0.9 4,182,668 - Psyr_3506 0.84 -1.0 4,182,668 - Psyr_3506 0.84 -0.5 4,182,668 - Psyr_3506 0.84 -0.4 4,182,668 - Psyr_3506 0.84 -0.1 4,182,668 - Psyr_3506 0.84 +0.3 4,182,668 - Psyr_3506 0.84 -3.9 4,182,668 - Psyr_3506 0.84 -0.1 4,182,889 + -1.7 4,182,890 - -1.0 4,182,996 - +0.9 4,183,091 + Psyr_3507 0.16 +0.4 4,183,091 + Psyr_3507 0.16 +0.8 4,183,091 + Psyr_3507 0.16 -2.7 4,183,207 - Psyr_3507 0.34 +0.4 4,183,286 + Psyr_3507 0.47 +2.0 4,183,286 + Psyr_3507 0.47 +2.1 4,183,286 + Psyr_3507 0.47 +0.0 4,183,460 + Psyr_3507 0.75 +1.0 4,183,461 - Psyr_3507 0.75 -0.1 4,183,614 + +0.5 4,183,614 + -0.6 4,183,670 + +0.4 4,183,681 + +0.8 4,183,682 - -1.3 4,183,682 - +1.0 4,183,694 - -0.4 4,183,694 - +0.1 4,183,720 + -0.6 4,183,727 - -2.1 4,183,727 - -0.6 4,183,855 + Psyr_3508 0.19 +0.6 4,183,855 + Psyr_3508 0.19 +1.1 4,184,248 + Psyr_3508 0.49 +1.0 4,184,255 - Psyr_3508 0.50 -1.0 4,184,255 - Psyr_3508 0.50 -0.4 4,184,255 - Psyr_3508 0.50 -0.2 4,184,284 + Psyr_3508 0.52 +1.1 4,184,334 + Psyr_3508 0.56 +2.6 4,184,343 - Psyr_3508 0.56 +0.0 4,184,464 + Psyr_3508 0.66 +1.2 4,184,464 + Psyr_3508 0.66 -1.2 4,184,464 + Psyr_3508 0.66 -0.6 4,184,465 - Psyr_3508 0.66 -1.2 4,184,465 - Psyr_3508 0.66 +0.9 4,184,527 + Psyr_3508 0.71 +1.2 4,184,527 + Psyr_3508 0.71 +0.1 4,184,527 + Psyr_3508 0.71 +2.0 4,184,527 + Psyr_3508 0.71 -1.4 4,184,527 + Psyr_3508 0.71 +2.4 4,184,528 - Psyr_3508 0.71 -3.3 4,184,528 - Psyr_3508 0.71 -0.3 4,184,528 - Psyr_3508 0.71 -1.2
Or see this region's nucleotide sequence