Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3232 and Psyr_3233 are separated by 250 nucleotides Psyr_3233 and Psyr_3234 are separated by 19 nucleotides Psyr_3234 and Psyr_3235 are separated by 124 nucleotides Psyr_3235 and Psyr_3236 are separated by 88 nucleotides
Psyr_3232: Psyr_3232 - Polysaccharide export protein, at 3,871,564 to 3,872,652
_3232
Psyr_3233: Psyr_3233 - FMN reductase, at 3,872,903 to 3,873,484
_3233
Psyr_3234: Psyr_3234 - Lysine exporter protein (LYSE/YGGA), at 3,873,504 to 3,874,112
_3234
Psyr_3235: Psyr_3235 - lipoprotein, putative, at 3,874,237 to 3,874,518
_3235
Psyr_3236: Psyr_3236 - transcriptional regulator, LysR family, at 3,874,607 to 3,875,497
_3236
Position (kb)
3873
3874
3875 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3872.625 kb on - strand at 3872.860 kb on + strand at 3872.860 kb on + strand at 3872.860 kb on + strand at 3872.860 kb on + strand at 3872.860 kb on + strand at 3872.860 kb on + strand at 3872.861 kb on - strand at 3872.861 kb on - strand at 3872.861 kb on - strand at 3872.861 kb on - strand at 3872.861 kb on - strand at 3872.861 kb on - strand at 3872.878 kb on + strand at 3872.878 kb on + strand at 3872.878 kb on + strand at 3872.878 kb on + strand at 3872.879 kb on - strand at 3872.879 kb on - strand at 3872.879 kb on - strand at 3872.879 kb on - strand at 3872.879 kb on - strand at 3872.958 kb on + strand at 3872.959 kb on - strand at 3873.004 kb on + strand, within Psyr_3233 at 3873.131 kb on + strand, within Psyr_3233 at 3873.131 kb on + strand, within Psyr_3233 at 3873.131 kb on + strand, within Psyr_3233 at 3873.132 kb on - strand, within Psyr_3233 at 3873.132 kb on - strand, within Psyr_3233 at 3873.354 kb on + strand, within Psyr_3233 at 3873.483 kb on - strand at 3873.483 kb on - strand at 3873.570 kb on - strand, within Psyr_3234 at 3873.570 kb on - strand, within Psyr_3234 at 3873.573 kb on + strand, within Psyr_3234 at 3873.685 kb on + strand, within Psyr_3234 at 3873.686 kb on - strand, within Psyr_3234 at 3873.691 kb on + strand, within Psyr_3234 at 3873.692 kb on - strand, within Psyr_3234 at 3873.710 kb on - strand, within Psyr_3234 at 3873.710 kb on - strand, within Psyr_3234 at 3873.710 kb on - strand, within Psyr_3234 at 3873.710 kb on - strand, within Psyr_3234 at 3873.853 kb on + strand, within Psyr_3234 at 3873.862 kb on + strand, within Psyr_3234 at 3873.863 kb on - strand, within Psyr_3234 at 3873.877 kb on + strand, within Psyr_3234 at 3873.891 kb on - strand, within Psyr_3234 at 3873.892 kb on + strand, within Psyr_3234 at 3873.892 kb on + strand, within Psyr_3234 at 3873.892 kb on + strand, within Psyr_3234 at 3873.893 kb on - strand, within Psyr_3234 at 3874.052 kb on + strand at 3874.052 kb on + strand at 3874.140 kb on + strand at 3874.140 kb on + strand at 3874.141 kb on - strand at 3874.239 kb on - strand at 3874.356 kb on - strand, within Psyr_3235 at 3874.509 kb on + strand at 3874.509 kb on + strand at 3874.510 kb on - strand at 3874.510 kb on - strand at 3874.510 kb on - strand at 3874.510 kb on - strand at 3874.510 kb on - strand at 3874.898 kb on + strand, within Psyr_3236 at 3874.898 kb on + strand, within Psyr_3236 at 3874.899 kb on - strand, within Psyr_3236 at 3875.016 kb on + strand, within Psyr_3236 at 3875.016 kb on + strand, within Psyr_3236 at 3875.016 kb on + strand, within Psyr_3236 at 3875.016 kb on + strand, within Psyr_3236 at 3875.016 kb on + strand, within Psyr_3236 at 3875.017 kb on - strand, within Psyr_3236 at 3875.017 kb on - strand, within Psyr_3236 at 3875.017 kb on - strand, within Psyr_3236 at 3875.017 kb on - strand, within Psyr_3236 at 3875.017 kb on - strand, within Psyr_3236
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 3,872,625 - -1.9 3,872,860 + +0.8 3,872,860 + +2.0 3,872,860 + -0.6 3,872,860 + -0.6 3,872,860 + -0.5 3,872,860 + +0.2 3,872,861 - -0.2 3,872,861 - +1.2 3,872,861 - -1.2 3,872,861 - +0.7 3,872,861 - -0.2 3,872,861 - -2.6 3,872,878 + -1.2 3,872,878 + +0.4 3,872,878 + +1.4 3,872,878 + -0.5 3,872,879 - -0.2 3,872,879 - -0.4 3,872,879 - +1.1 3,872,879 - -1.1 3,872,879 - +0.8 3,872,958 + +1.0 3,872,959 - +0.3 3,873,004 + Psyr_3233 0.17 +0.1 3,873,131 + Psyr_3233 0.39 -0.2 3,873,131 + Psyr_3233 0.39 +0.2 3,873,131 + Psyr_3233 0.39 +0.2 3,873,132 - Psyr_3233 0.39 +2.8 3,873,132 - Psyr_3233 0.39 -0.3 3,873,354 + Psyr_3233 0.77 +1.2 3,873,483 - +0.5 3,873,483 - -0.6 3,873,570 - Psyr_3234 0.11 +0.5 3,873,570 - Psyr_3234 0.11 +0.3 3,873,573 + Psyr_3234 0.11 +1.8 3,873,685 + Psyr_3234 0.30 +0.1 3,873,686 - Psyr_3234 0.30 -1.2 3,873,691 + Psyr_3234 0.31 -0.5 3,873,692 - Psyr_3234 0.31 -1.7 3,873,710 - Psyr_3234 0.34 +0.3 3,873,710 - Psyr_3234 0.34 +0.1 3,873,710 - Psyr_3234 0.34 -0.6 3,873,710 - Psyr_3234 0.34 +0.1 3,873,853 + Psyr_3234 0.57 +1.0 3,873,862 + Psyr_3234 0.59 +0.6 3,873,863 - Psyr_3234 0.59 -0.2 3,873,877 + Psyr_3234 0.61 -0.7 3,873,891 - Psyr_3234 0.64 +0.7 3,873,892 + Psyr_3234 0.64 +2.7 3,873,892 + Psyr_3234 0.64 +0.4 3,873,892 + Psyr_3234 0.64 -0.3 3,873,893 - Psyr_3234 0.64 -0.6 3,874,052 + -2.7 3,874,052 + +2.0 3,874,140 + -0.5 3,874,140 + +0.4 3,874,141 - -1.8 3,874,239 - -0.4 3,874,356 - Psyr_3235 0.42 -0.3 3,874,509 + +1.4 3,874,509 + +0.1 3,874,510 - -0.4 3,874,510 - -0.6 3,874,510 - -0.5 3,874,510 - -0.1 3,874,510 - -0.4 3,874,898 + Psyr_3236 0.33 +0.1 3,874,898 + Psyr_3236 0.33 -1.3 3,874,899 - Psyr_3236 0.33 -0.8 3,875,016 + Psyr_3236 0.46 -0.2 3,875,016 + Psyr_3236 0.46 +4.2 3,875,016 + Psyr_3236 0.46 +0.9 3,875,016 + Psyr_3236 0.46 +0.2 3,875,016 + Psyr_3236 0.46 +0.4 3,875,017 - Psyr_3236 0.46 +0.3 3,875,017 - Psyr_3236 0.46 +0.3 3,875,017 - Psyr_3236 0.46 -0.3 3,875,017 - Psyr_3236 0.46 -1.7 3,875,017 - Psyr_3236 0.46 +1.2
Or see this region's nucleotide sequence