Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3016 and Psyr_3017 are separated by 715 nucleotides Psyr_3017 and Psyr_3018 are separated by 10 nucleotides Psyr_3018 and Psyr_3019 overlap by 23 nucleotides
Psyr_3016: Psyr_3016 - Cobalamin synthesis protein/P47K:Cobalamin synthesis protein/P47K, at 3,612,306 to 3,613,370
_3016
Psyr_3017: Psyr_3017 - conserved hypothetical protein, at 3,614,086 to 3,614,268
_3017
Psyr_3018: Psyr_3018 - membrane protein, putative, at 3,614,279 to 3,615,001
_3018
Psyr_3019: Psyr_3019 - Cobalamin (vitamin B12) biosynthesis CbiG protein, at 3,614,979 to 3,615,428
_3019
Position (kb)
3614
3615 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3613.163 kb on - strand, within Psyr_3016 at 3613.163 kb on - strand, within Psyr_3016 at 3613.464 kb on + strand at 3613.550 kb on - strand at 3613.550 kb on - strand at 3613.661 kb on - strand at 3613.876 kb on + strand at 3613.876 kb on + strand at 3613.877 kb on - strand at 3613.877 kb on - strand at 3613.877 kb on - strand at 3613.974 kb on - strand at 3614.185 kb on + strand, within Psyr_3017 at 3614.185 kb on + strand, within Psyr_3017 at 3614.186 kb on - strand, within Psyr_3017 at 3614.297 kb on + strand at 3614.297 kb on + strand at 3614.298 kb on - strand at 3614.455 kb on + strand, within Psyr_3018 at 3614.456 kb on - strand, within Psyr_3018 at 3614.456 kb on - strand, within Psyr_3018 at 3614.567 kb on + strand at 3614.568 kb on - strand, within Psyr_3018 at 3614.568 kb on - strand, within Psyr_3018 at 3614.709 kb on + strand, within Psyr_3018 at 3614.709 kb on + strand, within Psyr_3018 at 3614.709 kb on + strand, within Psyr_3018 at 3614.710 kb on - strand, within Psyr_3018
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 3,613,163 - Psyr_3016 0.80 -0.8 3,613,163 - Psyr_3016 0.80 -1.4 3,613,464 + -0.8 3,613,550 - +0.6 3,613,550 - -1.2 3,613,661 - +0.1 3,613,876 + -1.7 3,613,876 + -0.5 3,613,877 - -2.8 3,613,877 - -0.0 3,613,877 - -0.9 3,613,974 - +0.9 3,614,185 + Psyr_3017 0.54 -0.0 3,614,185 + Psyr_3017 0.54 +0.4 3,614,186 - Psyr_3017 0.55 +0.7 3,614,297 + +0.2 3,614,297 + -0.6 3,614,298 - +0.1 3,614,455 + Psyr_3018 0.24 -2.7 3,614,456 - Psyr_3018 0.24 -1.7 3,614,456 - Psyr_3018 0.24 +0.2 3,614,567 + +0.4 3,614,568 - Psyr_3018 0.40 +0.2 3,614,568 - Psyr_3018 0.40 +0.8 3,614,709 + Psyr_3018 0.59 +3.0 3,614,709 + Psyr_3018 0.59 -0.3 3,614,709 + Psyr_3018 0.59 +2.4 3,614,710 - Psyr_3018 0.60 +0.1
Or see this region's nucleotide sequence