Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2849 and Psyr_2850 are separated by 82 nucleotides Psyr_2850 and Psyr_2851 are separated by 81 nucleotides Psyr_2851 and Psyr_2852 are separated by 298 nucleotides Psyr_2852 and Psyr_2853 are separated by 147 nucleotides
Psyr_2849: Psyr_2849 - CDS, at 3,422,438 to 3,423,085
_2849
Psyr_2850: Psyr_2850 - hypothetical protein, at 3,423,168 to 3,423,527
_2850
Psyr_2851: Psyr_2851 - conserved hypothetical protein, at 3,423,609 to 3,423,962
_2851
Psyr_2852: Psyr_2852 - conserved hypothetical protein, at 3,424,261 to 3,424,794
_2852
Psyr_2853: Psyr_2853 - CDS, at 3,424,942 to 3,425,439
_2853
Position (kb)
3423
3424 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3423.114 kb on + strand at 3423.146 kb on + strand at 3423.146 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.184 kb on + strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.185 kb on - strand at 3423.189 kb on - strand at 3423.189 kb on - strand at 3423.236 kb on - strand, within Psyr_2850 at 3423.337 kb on - strand, within Psyr_2850 at 3423.337 kb on - strand, within Psyr_2850 at 3423.337 kb on - strand, within Psyr_2850 at 3423.360 kb on + strand, within Psyr_2850 at 3423.360 kb on + strand, within Psyr_2850 at 3423.360 kb on + strand, within Psyr_2850 at 3423.360 kb on + strand, within Psyr_2850 at 3423.361 kb on - strand, within Psyr_2850 at 3423.361 kb on - strand, within Psyr_2850 at 3423.492 kb on + strand at 3423.594 kb on + strand at 3423.607 kb on + strand at 3423.826 kb on - strand, within Psyr_2851 at 3423.845 kb on - strand, within Psyr_2851 at 3423.873 kb on + strand, within Psyr_2851 at 3423.874 kb on - strand, within Psyr_2851 at 3423.993 kb on + strand at 3423.993 kb on + strand at 3424.083 kb on - strand at 3424.083 kb on - strand at 3424.083 kb on - strand at 3424.129 kb on + strand at 3424.130 kb on - strand at 3424.130 kb on - strand at 3424.130 kb on - strand at 3424.130 kb on - strand at 3424.130 kb on - strand at 3424.155 kb on + strand at 3424.156 kb on - strand at 3424.156 kb on - strand at 3424.192 kb on + strand at 3424.193 kb on - strand at 3424.193 kb on - strand at 3424.253 kb on + strand at 3424.268 kb on + strand at 3424.268 kb on + strand at 3424.268 kb on + strand at 3424.269 kb on - strand at 3424.269 kb on - strand at 3424.269 kb on - strand at 3424.276 kb on - strand at 3424.396 kb on + strand at 3424.397 kb on - strand, within Psyr_2852 at 3424.397 kb on - strand, within Psyr_2852 at 3424.397 kb on - strand, within Psyr_2852 at 3424.477 kb on + strand, within Psyr_2852 at 3424.477 kb on + strand, within Psyr_2852 at 3424.478 kb on - strand, within Psyr_2852 at 3424.478 kb on - strand, within Psyr_2852 at 3424.478 kb on - strand, within Psyr_2852 at 3424.478 kb on - strand, within Psyr_2852 at 3424.533 kb on + strand, within Psyr_2852 at 3424.559 kb on + strand, within Psyr_2852 at 3424.613 kb on - strand, within Psyr_2852 at 3424.774 kb on + strand at 3424.774 kb on + strand at 3424.860 kb on + strand at 3424.860 kb on + strand at 3424.861 kb on - strand at 3424.861 kb on - strand at 3424.861 kb on - strand at 3424.916 kb on - strand at 3424.916 kb on - strand at 3424.943 kb on + strand at 3424.943 kb on + strand at 3424.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 3,423,114 + +0.4 3,423,146 + +3.1 3,423,146 + -0.6 3,423,184 + +1.5 3,423,184 + +2.5 3,423,184 + +1.5 3,423,184 + +0.5 3,423,184 + -0.5 3,423,184 + -0.2 3,423,184 + +1.1 3,423,185 - -0.4 3,423,185 - +0.7 3,423,185 - +0.2 3,423,185 - +2.0 3,423,185 - +0.4 3,423,185 - -0.8 3,423,189 - +1.4 3,423,189 - +1.1 3,423,236 - Psyr_2850 0.19 +0.4 3,423,337 - Psyr_2850 0.47 -2.0 3,423,337 - Psyr_2850 0.47 +1.9 3,423,337 - Psyr_2850 0.47 +1.9 3,423,360 + Psyr_2850 0.53 +0.5 3,423,360 + Psyr_2850 0.53 +0.7 3,423,360 + Psyr_2850 0.53 -0.6 3,423,360 + Psyr_2850 0.53 +1.1 3,423,361 - Psyr_2850 0.54 -0.7 3,423,361 - Psyr_2850 0.54 +0.5 3,423,492 + -0.1 3,423,594 + +0.4 3,423,607 + -0.9 3,423,826 - Psyr_2851 0.61 +1.5 3,423,845 - Psyr_2851 0.67 +0.9 3,423,873 + Psyr_2851 0.75 -0.6 3,423,874 - Psyr_2851 0.75 +0.2 3,423,993 + +0.9 3,423,993 + -2.9 3,424,083 - -1.3 3,424,083 - -1.1 3,424,083 - +0.8 3,424,129 + +0.5 3,424,130 - +0.4 3,424,130 - +0.9 3,424,130 - -1.5 3,424,130 - -1.9 3,424,130 - +2.5 3,424,155 + -0.5 3,424,156 - +1.5 3,424,156 - -0.4 3,424,192 + +0.4 3,424,193 - -1.9 3,424,193 - +0.1 3,424,253 + -0.1 3,424,268 + -0.4 3,424,268 + -0.5 3,424,268 + -0.0 3,424,269 - +0.4 3,424,269 - -0.2 3,424,269 - +1.7 3,424,276 - -0.9 3,424,396 + -1.6 3,424,397 - Psyr_2852 0.25 +1.0 3,424,397 - Psyr_2852 0.25 -0.1 3,424,397 - Psyr_2852 0.25 -0.6 3,424,477 + Psyr_2852 0.40 -0.4 3,424,477 + Psyr_2852 0.40 +0.1 3,424,478 - Psyr_2852 0.41 +1.3 3,424,478 - Psyr_2852 0.41 +1.2 3,424,478 - Psyr_2852 0.41 -1.1 3,424,478 - Psyr_2852 0.41 -1.2 3,424,533 + Psyr_2852 0.51 +1.1 3,424,559 + Psyr_2852 0.56 +1.5 3,424,613 - Psyr_2852 0.66 -2.4 3,424,774 + -0.3 3,424,774 + -0.8 3,424,860 + +1.5 3,424,860 + +0.4 3,424,861 - +0.7 3,424,861 - +1.8 3,424,861 - -0.4 3,424,916 - +0.7 3,424,916 - -1.2 3,424,943 + -1.6 3,424,943 + -1.0 3,424,944 - -1.6
Or see this region's nucleotide sequence