Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2271 and Psyr_2272 are separated by 48 nucleotides Psyr_2272 and Psyr_2273 are separated by 288 nucleotides Psyr_2273 and Psyr_2274 are separated by 150 nucleotides
Psyr_2271: Psyr_2271 - membrane protein, putative, at 2,636,708 to 2,637,874
_2271
Psyr_2272: Psyr_2272 - Helix-turn-helix motif protein, at 2,637,923 to 2,638,528
_2272
Psyr_2273: Psyr_2273 - L-glutamine synthetase, at 2,638,817 to 2,640,151
_2273
Psyr_2274: Psyr_2274 - glutamate synthase (NADPH) GltB1 subunit, at 2,640,302 to 2,641,222
_2274
Position (kb)
2638
2639
2640
2641 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2637.866 kb on + strand at 2637.866 kb on + strand at 2637.873 kb on - strand at 2638.158 kb on + strand, within Psyr_2272 at 2638.158 kb on + strand, within Psyr_2272 at 2638.158 kb on + strand, within Psyr_2272 at 2638.158 kb on + strand, within Psyr_2272 at 2638.158 kb on + strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.207 kb on - strand, within Psyr_2272 at 2638.294 kb on - strand, within Psyr_2272 at 2638.483 kb on - strand at 2638.483 kb on - strand at 2638.617 kb on - strand at 2638.646 kb on + strand at 2638.647 kb on - strand at 2638.647 kb on - strand at 2638.647 kb on - strand at 2638.692 kb on - strand at 2638.749 kb on - strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.866 kb on - strand at 2638.869 kb on + strand at 2638.870 kb on - strand at 2639.127 kb on - strand, within Psyr_2273 at 2639.279 kb on + strand, within Psyr_2273 at 2639.280 kb on - strand, within Psyr_2273 at 2639.355 kb on - strand, within Psyr_2273 at 2639.399 kb on + strand, within Psyr_2273 at 2639.399 kb on + strand, within Psyr_2273 at 2639.399 kb on + strand, within Psyr_2273 at 2639.399 kb on + strand, within Psyr_2273 at 2639.399 kb on + strand, within Psyr_2273 at 2639.399 kb on + strand, within Psyr_2273 at 2639.399 kb on + strand, within Psyr_2273 at 2639.400 kb on - strand, within Psyr_2273 at 2639.400 kb on - strand, within Psyr_2273 at 2639.566 kb on + strand, within Psyr_2273 at 2639.627 kb on + strand, within Psyr_2273 at 2639.627 kb on + strand, within Psyr_2273 at 2639.699 kb on + strand, within Psyr_2273 at 2639.846 kb on + strand at 2640.098 kb on + strand at 2640.099 kb on - strand at 2640.135 kb on - strand at 2640.135 kb on - strand at 2640.219 kb on + strand at 2640.219 kb on + strand at 2640.219 kb on + strand at 2640.251 kb on - strand at 2640.251 kb on - strand at 2640.268 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.301 kb on + strand at 2640.302 kb on - strand at 2640.302 kb on - strand at 2640.302 kb on - strand at 2640.302 kb on - strand at 2640.302 kb on - strand at 2640.302 kb on - strand at 2640.302 kb on - strand at 2640.302 kb on - strand at 2640.425 kb on + strand, within Psyr_2274 at 2640.425 kb on + strand, within Psyr_2274 at 2640.425 kb on + strand, within Psyr_2274 at 2640.426 kb on - strand, within Psyr_2274 at 2640.426 kb on - strand, within Psyr_2274 at 2640.426 kb on - strand, within Psyr_2274 at 2640.426 kb on - strand, within Psyr_2274 at 2640.485 kb on + strand, within Psyr_2274 at 2640.485 kb on + strand, within Psyr_2274 at 2640.486 kb on - strand, within Psyr_2274 at 2641.003 kb on + strand, within Psyr_2274 at 2641.091 kb on + strand, within Psyr_2274 at 2641.091 kb on + strand, within Psyr_2274 at 2641.092 kb on - strand, within Psyr_2274 at 2641.092 kb on - strand, within Psyr_2274 at 2641.092 kb on - strand, within Psyr_2274 at 2641.092 kb on - strand, within Psyr_2274 at 2641.092 kb on - strand, within Psyr_2274
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 2,637,866 + +1.9 2,637,866 + -1.2 2,637,873 - -0.0 2,638,158 + Psyr_2272 0.39 +1.8 2,638,158 + Psyr_2272 0.39 -0.0 2,638,158 + Psyr_2272 0.39 +0.7 2,638,158 + Psyr_2272 0.39 -0.1 2,638,158 + Psyr_2272 0.39 -0.3 2,638,159 - Psyr_2272 0.39 +0.0 2,638,159 - Psyr_2272 0.39 -0.8 2,638,159 - Psyr_2272 0.39 +0.3 2,638,159 - Psyr_2272 0.39 +1.2 2,638,207 - Psyr_2272 0.47 +2.4 2,638,294 - Psyr_2272 0.61 -0.8 2,638,483 - +1.0 2,638,483 - +1.4 2,638,617 - -3.0 2,638,646 + -0.7 2,638,647 - -0.9 2,638,647 - -0.6 2,638,647 - -2.2 2,638,692 - +0.3 2,638,749 - -0.1 2,638,865 + -0.1 2,638,865 + -0.8 2,638,865 + +1.6 2,638,865 + +3.1 2,638,865 + +1.4 2,638,865 + -0.5 2,638,866 - +0.5 2,638,869 + -1.9 2,638,870 - +0.3 2,639,127 - Psyr_2273 0.23 -0.0 2,639,279 + Psyr_2273 0.35 +1.1 2,639,280 - Psyr_2273 0.35 -0.0 2,639,355 - Psyr_2273 0.40 +0.4 2,639,399 + Psyr_2273 0.44 +1.2 2,639,399 + Psyr_2273 0.44 +0.4 2,639,399 + Psyr_2273 0.44 +1.1 2,639,399 + Psyr_2273 0.44 +2.0 2,639,399 + Psyr_2273 0.44 +0.2 2,639,399 + Psyr_2273 0.44 +0.6 2,639,399 + Psyr_2273 0.44 +0.2 2,639,400 - Psyr_2273 0.44 -0.2 2,639,400 - Psyr_2273 0.44 -0.0 2,639,566 + Psyr_2273 0.56 -2.8 2,639,627 + Psyr_2273 0.61 +0.1 2,639,627 + Psyr_2273 0.61 +0.1 2,639,699 + Psyr_2273 0.66 +0.2 2,639,846 + +1.6 2,640,098 + +0.1 2,640,099 - +0.8 2,640,135 - +1.4 2,640,135 - -1.1 2,640,219 + -0.7 2,640,219 + +3.9 2,640,219 + -0.4 2,640,251 - -0.9 2,640,251 - -0.5 2,640,268 + -0.7 2,640,301 + -0.4 2,640,301 + +2.6 2,640,301 + +0.9 2,640,301 + +1.6 2,640,301 + -0.5 2,640,301 + +0.2 2,640,301 + -0.0 2,640,301 + -0.5 2,640,301 + -0.7 2,640,301 + +1.0 2,640,301 + +0.2 2,640,301 + -0.1 2,640,302 - -0.2 2,640,302 - -2.5 2,640,302 - -2.1 2,640,302 - +2.6 2,640,302 - +0.6 2,640,302 - +0.4 2,640,302 - -1.7 2,640,302 - +0.5 2,640,425 + Psyr_2274 0.13 -0.8 2,640,425 + Psyr_2274 0.13 -0.1 2,640,425 + Psyr_2274 0.13 -0.5 2,640,426 - Psyr_2274 0.13 -0.5 2,640,426 - Psyr_2274 0.13 +0.3 2,640,426 - Psyr_2274 0.13 -0.7 2,640,426 - Psyr_2274 0.13 -1.4 2,640,485 + Psyr_2274 0.20 +1.6 2,640,485 + Psyr_2274 0.20 -1.2 2,640,486 - Psyr_2274 0.20 -1.6 2,641,003 + Psyr_2274 0.76 -1.9 2,641,091 + Psyr_2274 0.86 -0.2 2,641,091 + Psyr_2274 0.86 -0.6 2,641,092 - Psyr_2274 0.86 -0.5 2,641,092 - Psyr_2274 0.86 +0.9 2,641,092 - Psyr_2274 0.86 +2.2 2,641,092 - Psyr_2274 0.86 -1.0 2,641,092 - Psyr_2274 0.86 -0.8
Or see this region's nucleotide sequence