Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2140 and Psyr_2141 are separated by 19 nucleotides Psyr_2141 and Psyr_2142 are separated by 225 nucleotides Psyr_2142 and Psyr_2143 are separated by 259 nucleotides
Psyr_2140: Psyr_2140 - conserved hypothetical protein, at 2,500,040 to 2,500,228
_2140
Psyr_2141: Psyr_2141 - hypothetical protein, at 2,500,248 to 2,500,640
_2141
Psyr_2142: Psyr_2142 - FecR protein, at 2,500,866 to 2,501,894
_2142
Psyr_2143: Psyr_2143 - delta1-piperideine 2-carboxylate reductase, at 2,502,154 to 2,503,185
_2143
Position (kb)
2500
2501
2502 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2499.906 kb on + strand at 2499.906 kb on + strand at 2499.984 kb on + strand at 2499.985 kb on - strand at 2499.985 kb on - strand at 2500.157 kb on + strand, within Psyr_2140 at 2500.157 kb on + strand, within Psyr_2140 at 2500.157 kb on + strand, within Psyr_2140 at 2500.158 kb on - strand, within Psyr_2140 at 2500.158 kb on - strand, within Psyr_2140 at 2500.158 kb on - strand, within Psyr_2140 at 2500.158 kb on - strand, within Psyr_2140 at 2500.158 kb on - strand, within Psyr_2140 at 2500.226 kb on + strand at 2500.227 kb on - strand at 2500.309 kb on + strand, within Psyr_2141 at 2500.309 kb on + strand, within Psyr_2141 at 2500.309 kb on + strand, within Psyr_2141 at 2500.310 kb on - strand, within Psyr_2141 at 2500.310 kb on - strand, within Psyr_2141 at 2500.420 kb on - strand, within Psyr_2141 at 2500.665 kb on + strand at 2500.679 kb on + strand at 2500.679 kb on + strand at 2500.679 kb on + strand at 2500.679 kb on + strand at 2500.680 kb on - strand at 2500.680 kb on - strand at 2500.682 kb on + strand at 2500.683 kb on - strand at 2500.683 kb on - strand at 2500.683 kb on - strand at 2500.713 kb on - strand at 2500.778 kb on - strand at 2500.933 kb on + strand at 2500.933 kb on + strand at 2500.933 kb on + strand at 2500.933 kb on + strand at 2500.933 kb on + strand at 2500.933 kb on + strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.934 kb on - strand at 2500.990 kb on + strand, within Psyr_2142 at 2500.991 kb on - strand, within Psyr_2142 at 2500.991 kb on - strand, within Psyr_2142 at 2501.182 kb on + strand, within Psyr_2142 at 2501.183 kb on - strand, within Psyr_2142 at 2501.290 kb on + strand, within Psyr_2142 at 2501.290 kb on + strand, within Psyr_2142 at 2501.290 kb on + strand, within Psyr_2142 at 2501.290 kb on + strand, within Psyr_2142 at 2501.291 kb on - strand, within Psyr_2142 at 2501.291 kb on - strand, within Psyr_2142 at 2501.298 kb on + strand, within Psyr_2142 at 2501.298 kb on + strand, within Psyr_2142 at 2501.298 kb on + strand, within Psyr_2142 at 2501.298 kb on + strand, within Psyr_2142 at 2501.298 kb on + strand, within Psyr_2142 at 2501.298 kb on + strand, within Psyr_2142 at 2501.299 kb on - strand, within Psyr_2142 at 2501.299 kb on - strand at 2501.299 kb on - strand, within Psyr_2142 at 2501.299 kb on - strand, within Psyr_2142 at 2501.299 kb on - strand, within Psyr_2142 at 2501.299 kb on - strand, within Psyr_2142 at 2501.299 kb on - strand, within Psyr_2142 at 2501.437 kb on + strand, within Psyr_2142 at 2501.518 kb on + strand, within Psyr_2142 at 2501.519 kb on - strand, within Psyr_2142 at 2501.519 kb on - strand, within Psyr_2142 at 2501.519 kb on - strand, within Psyr_2142 at 2501.519 kb on - strand, within Psyr_2142 at 2501.527 kb on + strand, within Psyr_2142 at 2501.740 kb on + strand, within Psyr_2142 at 2501.740 kb on + strand, within Psyr_2142 at 2501.741 kb on - strand, within Psyr_2142 at 2501.741 kb on - strand, within Psyr_2142 at 2501.741 kb on - strand, within Psyr_2142 at 2501.908 kb on + strand at 2501.908 kb on + strand at 2501.909 kb on - strand at 2501.909 kb on - strand at 2501.909 kb on - strand at 2501.910 kb on + strand at 2501.910 kb on + strand at 2501.910 kb on + strand at 2501.910 kb on + strand at 2501.910 kb on + strand at 2501.926 kb on + strand at 2501.985 kb on + strand at 2502.044 kb on - strand at 2502.080 kb on + strand at 2502.081 kb on - strand at 2502.130 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 2,499,906 + -0.0 2,499,906 + +0.0 2,499,984 + -1.8 2,499,985 - +1.2 2,499,985 - -1.1 2,500,157 + Psyr_2140 0.62 +0.2 2,500,157 + Psyr_2140 0.62 +1.6 2,500,157 + Psyr_2140 0.62 +0.5 2,500,158 - Psyr_2140 0.62 -2.2 2,500,158 - Psyr_2140 0.62 -1.7 2,500,158 - Psyr_2140 0.62 -0.6 2,500,158 - Psyr_2140 0.62 +0.9 2,500,158 - Psyr_2140 0.62 -1.3 2,500,226 + -0.3 2,500,227 - -1.0 2,500,309 + Psyr_2141 0.16 -1.5 2,500,309 + Psyr_2141 0.16 -1.1 2,500,309 + Psyr_2141 0.16 -0.7 2,500,310 - Psyr_2141 0.16 -0.3 2,500,310 - Psyr_2141 0.16 +0.3 2,500,420 - Psyr_2141 0.44 -1.3 2,500,665 + -2.4 2,500,679 + -2.2 2,500,679 + -1.5 2,500,679 + -1.3 2,500,679 + +0.7 2,500,680 - -0.6 2,500,680 - +1.1 2,500,682 + +1.5 2,500,683 - -2.6 2,500,683 - +1.1 2,500,683 - +1.2 2,500,713 - +1.0 2,500,778 - +2.5 2,500,933 + -2.0 2,500,933 + +0.2 2,500,933 + +1.2 2,500,933 + +1.0 2,500,933 + -0.2 2,500,933 + -1.5 2,500,934 - +3.1 2,500,934 - +1.7 2,500,934 - -2.6 2,500,934 - +0.0 2,500,934 - +1.5 2,500,934 - -1.3 2,500,934 - +3.8 2,500,934 - +1.7 2,500,934 - +0.0 2,500,934 - -0.5 2,500,990 + Psyr_2142 0.12 +0.5 2,500,991 - Psyr_2142 0.12 +3.1 2,500,991 - Psyr_2142 0.12 -0.4 2,501,182 + Psyr_2142 0.31 -2.2 2,501,183 - Psyr_2142 0.31 -0.3 2,501,290 + Psyr_2142 0.41 -0.5 2,501,290 + Psyr_2142 0.41 +0.0 2,501,290 + Psyr_2142 0.41 -0.4 2,501,290 + Psyr_2142 0.41 -1.3 2,501,291 - Psyr_2142 0.41 -1.4 2,501,291 - Psyr_2142 0.41 +0.8 2,501,298 + Psyr_2142 0.42 +0.4 2,501,298 + Psyr_2142 0.42 -1.9 2,501,298 + Psyr_2142 0.42 +0.2 2,501,298 + Psyr_2142 0.42 -0.1 2,501,298 + Psyr_2142 0.42 -0.9 2,501,298 + Psyr_2142 0.42 -0.2 2,501,299 - Psyr_2142 0.42 +0.5 2,501,299 - +2.1 2,501,299 - Psyr_2142 0.42 -0.8 2,501,299 - Psyr_2142 0.42 -1.3 2,501,299 - Psyr_2142 0.42 -0.2 2,501,299 - Psyr_2142 0.42 -1.2 2,501,299 - Psyr_2142 0.42 +0.2 2,501,437 + Psyr_2142 0.55 -0.7 2,501,518 + Psyr_2142 0.63 -1.2 2,501,519 - Psyr_2142 0.63 -2.2 2,501,519 - Psyr_2142 0.63 -0.4 2,501,519 - Psyr_2142 0.63 +1.7 2,501,519 - Psyr_2142 0.63 -1.3 2,501,527 + Psyr_2142 0.64 -1.1 2,501,740 + Psyr_2142 0.85 -1.4 2,501,740 + Psyr_2142 0.85 -1.1 2,501,741 - Psyr_2142 0.85 -1.1 2,501,741 - Psyr_2142 0.85 +0.2 2,501,741 - Psyr_2142 0.85 +0.2 2,501,908 + +1.0 2,501,908 + -0.8 2,501,909 - -0.3 2,501,909 - +0.9 2,501,909 - +0.1 2,501,910 + +1.3 2,501,910 + -0.3 2,501,910 + -0.3 2,501,910 + -0.4 2,501,910 + -0.1 2,501,926 + -0.2 2,501,985 + +1.8 2,502,044 - +0.6 2,502,080 + -1.3 2,502,081 - -2.6 2,502,130 + +0.1
Or see this region's nucleotide sequence