Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1084 and Psyr_1085 are separated by 583 nucleotides Psyr_1085 and Psyr_1086 are separated by 156 nucleotides
Psyr_1084: Psyr_1084 - Histone-like bacterial DNA-binding protein, at 1,225,706 to 1,225,987
_1084
Psyr_1085: Psyr_1085 - 23S rRNA m(6)A-1618 methyltransferase, at 1,226,571 to 1,227,557
_1085
Psyr_1086: Psyr_1086 - valyl-tRNA synthetase, at 1,227,714 to 1,230,560
_1086
Position (kb) 1226
1227
1228 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1225.589 kb on + strand at 1225.590 kb on - strand at 1225.593 kb on + strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.594 kb on - strand at 1225.607 kb on + strand at 1225.608 kb on - strand at 1225.714 kb on + strand at 1225.714 kb on + strand at 1225.715 kb on - strand at 1225.715 kb on - strand at 1225.715 kb on - strand at 1225.718 kb on - strand at 1225.718 kb on - strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.985 kb on + strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1225.986 kb on - strand at 1226.184 kb on + strand at 1226.185 kb on - strand at 1226.185 kb on - strand at 1226.242 kb on + strand at 1226.282 kb on + strand at 1226.321 kb on - strand at 1226.547 kb on - strand at 1226.653 kb on - strand at 1226.653 kb on - strand at 1227.115 kb on + strand, within Psyr_1085 at 1227.123 kb on - strand, within Psyr_1085 at 1227.140 kb on - strand, within Psyr_1085 at 1227.245 kb on - strand, within Psyr_1085 at 1227.245 kb on - strand, within Psyr_1085 at 1227.245 kb on - strand, within Psyr_1085 at 1227.277 kb on + strand, within Psyr_1085 at 1227.278 kb on - strand, within Psyr_1085 at 1227.311 kb on - strand, within Psyr_1085 at 1227.311 kb on - strand, within Psyr_1085 at 1227.648 kb on + strand at 1227.648 kb on + strand at 1227.648 kb on + strand at 1227.649 kb on - strand at 1227.656 kb on + strand at 1227.670 kb on + strand at 1227.715 kb on + strand at 1227.716 kb on - strand at 1227.716 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 1,225,589 + -1.2 1,225,590 - -0.0 1,225,593 + +0.3 1,225,594 - -2.5 1,225,594 - -0.7 1,225,594 - -1.4 1,225,594 - +2.0 1,225,594 - +0.2 1,225,594 - -0.7 1,225,594 - +0.4 1,225,594 - +0.6 1,225,607 + -0.0 1,225,608 - +0.5 1,225,714 + -0.8 1,225,714 + -0.7 1,225,715 - +2.0 1,225,715 - +0.0 1,225,715 - +1.3 1,225,718 - +3.2 1,225,718 - -2.4 1,225,985 + -0.2 1,225,985 + -0.5 1,225,985 + +0.0 1,225,985 + -0.7 1,225,985 + -0.2 1,225,985 + +0.2 1,225,985 + -0.8 1,225,985 + +0.5 1,225,985 + +1.3 1,225,985 + +0.8 1,225,985 + +0.4 1,225,985 + -1.2 1,225,985 + +1.0 1,225,985 + -0.6 1,225,986 - +0.5 1,225,986 - -1.1 1,225,986 - -0.6 1,225,986 - +0.6 1,225,986 - -0.4 1,225,986 - -2.6 1,225,986 - -1.1 1,226,184 + +1.6 1,226,185 - +1.0 1,226,185 - +0.6 1,226,242 + -0.5 1,226,282 + -0.8 1,226,321 - -0.6 1,226,547 - +1.9 1,226,653 - -1.0 1,226,653 - -0.3 1,227,115 + Psyr_1085 0.55 -0.4 1,227,123 - Psyr_1085 0.56 -0.4 1,227,140 - Psyr_1085 0.58 +0.5 1,227,245 - Psyr_1085 0.68 +0.3 1,227,245 - Psyr_1085 0.68 +0.2 1,227,245 - Psyr_1085 0.68 -0.2 1,227,277 + Psyr_1085 0.72 +0.8 1,227,278 - Psyr_1085 0.72 +1.4 1,227,311 - Psyr_1085 0.75 +2.2 1,227,311 - Psyr_1085 0.75 +1.1 1,227,648 + +0.8 1,227,648 + -1.4 1,227,648 + +0.1 1,227,649 - -0.1 1,227,656 + -0.4 1,227,670 + +0.0 1,227,715 + -1.4 1,227,716 - +0.9 1,227,716 - -0.3
Or see this region's nucleotide sequence