Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1074 and Psyr_1075 are separated by 10 nucleotides Psyr_1075 and Psyr_1076 are separated by 153 nucleotides Psyr_1076 and Psyr_1077 are separated by 42 nucleotides
Psyr_1074: Psyr_1074 - amino acid ABC transporter membrane protein 2, PAAT family, at 1,217,239 to 1,218,336
_1074
Psyr_1075: Psyr_1075 - amino acid ABC transporter ATP-binding protein, PAAT family, at 1,218,347 to 1,219,111
_1075
Psyr_1076: Psyr_1076 - Cyanate transport system protein, at 1,219,265 to 1,220,509
_1076
Psyr_1077: Psyr_1077 - Isoflavone reductase, at 1,220,552 to 1,221,490
_1077
Position (kb) 1219
1220
1221 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1218.280 kb on - strand at 1218.309 kb on + strand at 1218.309 kb on + strand at 1218.309 kb on + strand at 1218.309 kb on + strand at 1218.309 kb on + strand at 1218.310 kb on - strand at 1218.310 kb on - strand at 1218.310 kb on - strand at 1218.310 kb on - strand at 1218.310 kb on - strand at 1218.310 kb on - strand at 1218.310 kb on - strand at 1218.358 kb on + strand at 1218.358 kb on + strand at 1218.364 kb on + strand at 1218.364 kb on + strand at 1218.406 kb on - strand at 1218.406 kb on - strand at 1218.417 kb on - strand at 1218.432 kb on + strand, within Psyr_1075 at 1218.432 kb on + strand, within Psyr_1075 at 1218.432 kb on + strand, within Psyr_1075 at 1218.432 kb on + strand, within Psyr_1075 at 1218.433 kb on - strand, within Psyr_1075 at 1218.433 kb on - strand, within Psyr_1075 at 1218.433 kb on - strand, within Psyr_1075 at 1218.433 kb on - strand, within Psyr_1075 at 1218.472 kb on + strand, within Psyr_1075 at 1218.473 kb on - strand, within Psyr_1075 at 1218.744 kb on - strand, within Psyr_1075 at 1218.744 kb on - strand, within Psyr_1075 at 1219.079 kb on - strand at 1219.161 kb on - strand at 1219.186 kb on + strand at 1219.186 kb on + strand at 1219.186 kb on + strand at 1219.186 kb on + strand at 1219.186 kb on + strand at 1219.186 kb on + strand at 1219.186 kb on + strand at 1219.186 kb on + strand at 1219.187 kb on - strand at 1219.187 kb on - strand at 1219.187 kb on - strand at 1219.187 kb on - strand at 1219.208 kb on + strand at 1219.208 kb on + strand at 1219.208 kb on + strand at 1219.208 kb on + strand at 1219.209 kb on - strand at 1219.209 kb on - strand at 1219.259 kb on + strand at 1219.259 kb on + strand at 1219.259 kb on + strand at 1219.259 kb on + strand at 1219.259 kb on + strand at 1219.259 kb on + strand at 1219.260 kb on - strand at 1219.260 kb on - strand at 1219.260 kb on - strand at 1219.260 kb on - strand at 1219.733 kb on + strand, within Psyr_1076 at 1219.733 kb on + strand, within Psyr_1076 at 1219.733 kb on + strand, within Psyr_1076 at 1219.734 kb on - strand, within Psyr_1076 at 1219.796 kb on + strand, within Psyr_1076 at 1219.796 kb on + strand, within Psyr_1076 at 1219.796 kb on + strand, within Psyr_1076 at 1219.961 kb on + strand, within Psyr_1076 at 1219.961 kb on + strand, within Psyr_1076 at 1219.961 kb on + strand, within Psyr_1076 at 1219.961 kb on + strand, within Psyr_1076 at 1219.961 kb on + strand, within Psyr_1076 at 1219.961 kb on + strand, within Psyr_1076 at 1219.961 kb on + strand, within Psyr_1076 at 1219.961 kb on + strand, within Psyr_1076 at 1219.961 kb on + strand, within Psyr_1076 at 1219.962 kb on - strand, within Psyr_1076 at 1219.962 kb on - strand, within Psyr_1076 at 1219.962 kb on - strand, within Psyr_1076 at 1219.962 kb on - strand, within Psyr_1076 at 1219.962 kb on - strand, within Psyr_1076 at 1219.962 kb on - strand, within Psyr_1076 at 1219.962 kb on - strand, within Psyr_1076 at 1219.988 kb on + strand, within Psyr_1076 at 1219.989 kb on - strand, within Psyr_1076 at 1219.989 kb on - strand, within Psyr_1076 at 1219.989 kb on - strand, within Psyr_1076 at 1219.989 kb on - strand, within Psyr_1076 at 1220.108 kb on + strand, within Psyr_1076 at 1220.109 kb on - strand, within Psyr_1076 at 1220.192 kb on + strand, within Psyr_1076 at 1220.343 kb on - strand, within Psyr_1076 at 1220.622 kb on + strand at 1220.623 kb on - strand at 1220.623 kb on - strand at 1220.623 kb on - strand at 1220.623 kb on - strand at 1220.623 kb on - strand at 1220.623 kb on - strand at 1220.623 kb on - strand at 1220.623 kb on - strand at 1220.623 kb on - strand at 1220.738 kb on + strand, within Psyr_1077 at 1220.739 kb on - strand, within Psyr_1077 at 1220.796 kb on + strand, within Psyr_1077 at 1220.796 kb on + strand, within Psyr_1077 at 1220.796 kb on + strand, within Psyr_1077 at 1220.797 kb on - strand, within Psyr_1077 at 1220.820 kb on + strand, within Psyr_1077 at 1220.820 kb on + strand, within Psyr_1077 at 1220.820 kb on + strand, within Psyr_1077 at 1220.821 kb on - strand, within Psyr_1077 at 1220.821 kb on - strand, within Psyr_1077 at 1220.821 kb on - strand, within Psyr_1077 at 1220.821 kb on - strand, within Psyr_1077 at 1220.989 kb on + strand, within Psyr_1077 at 1220.989 kb on + strand, within Psyr_1077 at 1220.990 kb on - strand, within Psyr_1077 at 1220.990 kb on - strand, within Psyr_1077 at 1220.990 kb on - strand, within Psyr_1077 at 1220.990 kb on - strand, within Psyr_1077 at 1221.106 kb on - strand, within Psyr_1077 at 1221.140 kb on + strand, within Psyr_1077 at 1221.141 kb on - strand, within Psyr_1077 at 1221.141 kb on - strand, within Psyr_1077 at 1221.141 kb on - strand, within Psyr_1077 at 1221.226 kb on - strand, within Psyr_1077 at 1221.343 kb on + strand, within Psyr_1077 at 1221.343 kb on + strand, within Psyr_1077 at 1221.362 kb on + strand, within Psyr_1077 at 1221.446 kb on + strand at 1221.446 kb on + strand at 1221.446 kb on + strand at 1221.446 kb on + strand at 1221.446 kb on + strand at 1221.446 kb on + strand at 1221.446 kb on + strand at 1221.446 kb on + strand at 1221.446 kb on + strand at 1221.447 kb on - strand at 1221.447 kb on - strand at 1221.447 kb on - strand at 1221.447 kb on - strand at 1221.447 kb on - strand at 1221.447 kb on - strand at 1221.447 kb on - strand at 1221.447 kb on - strand at 1221.447 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 1,218,280 - -1.1 1,218,309 + +0.4 1,218,309 + -0.3 1,218,309 + +0.1 1,218,309 + +1.2 1,218,309 + +0.6 1,218,310 - -0.8 1,218,310 - +0.0 1,218,310 - -0.9 1,218,310 - -1.2 1,218,310 - +0.5 1,218,310 - +0.4 1,218,310 - -0.5 1,218,358 + +1.6 1,218,358 + -1.3 1,218,364 + +0.2 1,218,364 + -0.9 1,218,406 - +0.6 1,218,406 - -1.4 1,218,417 - -0.1 1,218,432 + Psyr_1075 0.11 +0.3 1,218,432 + Psyr_1075 0.11 +0.1 1,218,432 + Psyr_1075 0.11 +0.9 1,218,432 + Psyr_1075 0.11 +0.1 1,218,433 - Psyr_1075 0.11 -2.1 1,218,433 - Psyr_1075 0.11 -0.1 1,218,433 - Psyr_1075 0.11 +0.7 1,218,433 - Psyr_1075 0.11 -1.8 1,218,472 + Psyr_1075 0.16 +1.6 1,218,473 - Psyr_1075 0.16 -0.8 1,218,744 - Psyr_1075 0.52 -1.1 1,218,744 - Psyr_1075 0.52 +0.8 1,219,079 - -0.4 1,219,161 - -0.4 1,219,186 + -2.6 1,219,186 + +0.3 1,219,186 + +1.1 1,219,186 + -0.6 1,219,186 + +0.4 1,219,186 + -0.3 1,219,186 + +1.1 1,219,186 + -0.7 1,219,187 - -3.1 1,219,187 - -1.5 1,219,187 - -0.5 1,219,187 - -0.8 1,219,208 + +0.2 1,219,208 + -0.3 1,219,208 + +0.5 1,219,208 + -0.7 1,219,209 - -1.3 1,219,209 - -1.2 1,219,259 + -0.4 1,219,259 + -0.4 1,219,259 + -0.1 1,219,259 + +0.3 1,219,259 + -0.4 1,219,259 + +2.8 1,219,260 - -0.3 1,219,260 - -0.5 1,219,260 - -2.1 1,219,260 - +0.7 1,219,733 + Psyr_1076 0.38 -0.2 1,219,733 + Psyr_1076 0.38 +0.9 1,219,733 + Psyr_1076 0.38 +1.2 1,219,734 - Psyr_1076 0.38 +1.2 1,219,796 + Psyr_1076 0.43 -0.3 1,219,796 + Psyr_1076 0.43 +1.0 1,219,796 + Psyr_1076 0.43 -0.7 1,219,961 + Psyr_1076 0.56 -0.4 1,219,961 + Psyr_1076 0.56 +1.6 1,219,961 + Psyr_1076 0.56 +1.4 1,219,961 + Psyr_1076 0.56 +0.7 1,219,961 + Psyr_1076 0.56 +0.9 1,219,961 + Psyr_1076 0.56 -1.1 1,219,961 + Psyr_1076 0.56 +0.1 1,219,961 + Psyr_1076 0.56 -0.5 1,219,961 + Psyr_1076 0.56 +0.1 1,219,962 - Psyr_1076 0.56 -0.9 1,219,962 - Psyr_1076 0.56 -0.4 1,219,962 - Psyr_1076 0.56 -0.3 1,219,962 - Psyr_1076 0.56 +1.4 1,219,962 - Psyr_1076 0.56 +0.6 1,219,962 - Psyr_1076 0.56 -1.2 1,219,962 - Psyr_1076 0.56 +1.1 1,219,988 + Psyr_1076 0.58 +2.0 1,219,989 - Psyr_1076 0.58 -0.2 1,219,989 - Psyr_1076 0.58 -0.8 1,219,989 - Psyr_1076 0.58 +1.0 1,219,989 - Psyr_1076 0.58 +1.1 1,220,108 + Psyr_1076 0.68 -0.3 1,220,109 - Psyr_1076 0.68 -0.6 1,220,192 + Psyr_1076 0.74 +0.3 1,220,343 - Psyr_1076 0.87 +0.1 1,220,622 + +1.6 1,220,623 - +0.2 1,220,623 - -1.2 1,220,623 - +0.2 1,220,623 - +1.0 1,220,623 - +1.6 1,220,623 - -1.6 1,220,623 - +0.1 1,220,623 - -0.2 1,220,623 - -0.8 1,220,738 + Psyr_1077 0.20 +1.6 1,220,739 - Psyr_1077 0.20 -2.1 1,220,796 + Psyr_1077 0.26 -0.2 1,220,796 + Psyr_1077 0.26 +2.4 1,220,796 + Psyr_1077 0.26 -0.7 1,220,797 - Psyr_1077 0.26 +1.3 1,220,820 + Psyr_1077 0.29 -0.4 1,220,820 + Psyr_1077 0.29 -2.3 1,220,820 + Psyr_1077 0.29 +0.6 1,220,821 - Psyr_1077 0.29 +1.8 1,220,821 - Psyr_1077 0.29 +2.6 1,220,821 - Psyr_1077 0.29 +1.6 1,220,821 - Psyr_1077 0.29 -1.7 1,220,989 + Psyr_1077 0.47 -0.4 1,220,989 + Psyr_1077 0.47 +0.3 1,220,990 - Psyr_1077 0.47 +0.7 1,220,990 - Psyr_1077 0.47 +0.1 1,220,990 - Psyr_1077 0.47 -0.1 1,220,990 - Psyr_1077 0.47 +2.6 1,221,106 - Psyr_1077 0.59 +0.3 1,221,140 + Psyr_1077 0.63 +1.6 1,221,141 - Psyr_1077 0.63 -1.3 1,221,141 - Psyr_1077 0.63 +0.1 1,221,141 - Psyr_1077 0.63 +0.7 1,221,226 - Psyr_1077 0.72 -0.9 1,221,343 + Psyr_1077 0.84 +0.6 1,221,343 + Psyr_1077 0.84 -0.6 1,221,362 + Psyr_1077 0.86 -0.7 1,221,446 + -0.1 1,221,446 + +1.0 1,221,446 + -2.8 1,221,446 + +0.5 1,221,446 + +1.8 1,221,446 + -1.0 1,221,446 + +0.9 1,221,446 + -0.1 1,221,446 + -0.7 1,221,447 - -0.1 1,221,447 - -0.1 1,221,447 - -2.7 1,221,447 - -0.6 1,221,447 - -1.8 1,221,447 - -0.6 1,221,447 - -0.1 1,221,447 - +1.9 1,221,447 - -0.3
Or see this region's nucleotide sequence