Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0984 and Psyr_0985 are separated by 54 nucleotides Psyr_0985 and Psyr_0986 are separated by 246 nucleotides Psyr_0986 and Psyr_0987 are separated by 352 nucleotides Psyr_0987 and Psyr_0988 are separated by 59 nucleotides
Psyr_0984: Psyr_0984 - Peptidase M48, Ste24p, at 1,114,740 to 1,115,588
_0984
Psyr_0985: Psyr_0985 - Protein of unknown function UPF0016, at 1,115,643 to 1,116,227
_0985
Psyr_0986: Psyr_0986 - 16S rRNA m(2)G 1207 methyltransferase, at 1,116,474 to 1,117,472
_0986
Psyr_0987: Psyr_0987 - Helix-turn-helix, Fis-type, at 1,117,825 to 1,118,124
_0987
Psyr_0988: Psyr_0988 - Integrase, catalytic region, at 1,118,184 to 1,118,966
_0988
Position (kb)
1116
1117
1118 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1115.566 kb on - strand at 1115.580 kb on - strand at 1115.606 kb on - strand at 1115.632 kb on - strand at 1115.644 kb on + strand at 1115.645 kb on - strand at 1115.645 kb on - strand at 1115.645 kb on - strand at 1115.645 kb on - strand at 1115.645 kb on - strand at 1115.645 kb on - strand at 1115.674 kb on + strand at 1115.674 kb on + strand at 1115.674 kb on + strand at 1115.675 kb on - strand at 1115.830 kb on + strand, within Psyr_0985 at 1115.831 kb on - strand, within Psyr_0985 at 1115.831 kb on - strand, within Psyr_0985 at 1115.831 kb on - strand, within Psyr_0985 at 1115.831 kb on - strand, within Psyr_0985 at 1115.831 kb on - strand, within Psyr_0985 at 1115.831 kb on - strand, within Psyr_0985 at 1115.836 kb on + strand, within Psyr_0985 at 1115.836 kb on + strand, within Psyr_0985 at 1115.836 kb on + strand, within Psyr_0985 at 1115.837 kb on - strand, within Psyr_0985 at 1115.837 kb on - strand, within Psyr_0985 at 1116.040 kb on + strand, within Psyr_0985 at 1116.040 kb on + strand, within Psyr_0985 at 1116.210 kb on + strand at 1116.211 kb on - strand at 1116.211 kb on - strand at 1116.258 kb on + strand at 1116.267 kb on + strand at 1116.267 kb on + strand at 1116.268 kb on - strand at 1116.268 kb on - strand at 1116.268 kb on - strand at 1116.268 kb on - strand at 1116.268 kb on - strand at 1116.303 kb on + strand at 1116.303 kb on + strand at 1116.303 kb on + strand at 1116.304 kb on - strand at 1116.304 kb on - strand at 1116.304 kb on - strand at 1116.304 kb on - strand at 1116.352 kb on + strand at 1116.352 kb on + strand at 1116.352 kb on + strand at 1116.352 kb on + strand at 1116.352 kb on + strand at 1116.352 kb on + strand at 1116.352 kb on + strand at 1116.353 kb on - strand at 1116.353 kb on - strand at 1116.353 kb on - strand at 1116.353 kb on - strand at 1116.353 kb on - strand at 1116.373 kb on + strand at 1116.373 kb on + strand at 1116.373 kb on + strand at 1116.373 kb on + strand at 1116.373 kb on + strand at 1116.373 kb on + strand at 1116.373 kb on + strand at 1116.373 kb on + strand at 1116.373 kb on + strand at 1116.373 kb on + strand at 1116.373 kb on + strand at 1116.374 kb on - strand at 1116.374 kb on - strand at 1116.374 kb on - strand at 1116.374 kb on - strand at 1116.374 kb on - strand at 1116.374 kb on - strand at 1116.374 kb on - strand at 1116.374 kb on - strand at 1116.374 kb on - strand at 1116.374 kb on - strand at 1116.374 kb on - strand at 1116.374 kb on - strand at 1116.374 kb on - strand at 1116.382 kb on - strand at 1116.382 kb on - strand at 1116.382 kb on - strand at 1116.382 kb on - strand at 1116.382 kb on - strand at 1116.415 kb on + strand at 1116.415 kb on + strand at 1116.416 kb on - strand at 1116.470 kb on + strand at 1116.470 kb on + strand at 1116.470 kb on + strand at 1116.471 kb on - strand at 1116.493 kb on + strand at 1116.494 kb on - strand at 1116.494 kb on - strand at 1116.494 kb on - strand at 1116.576 kb on - strand, within Psyr_0986 at 1116.652 kb on + strand, within Psyr_0986 at 1116.652 kb on + strand, within Psyr_0986 at 1116.653 kb on - strand, within Psyr_0986 at 1116.653 kb on - strand, within Psyr_0986 at 1116.823 kb on + strand, within Psyr_0986 at 1117.223 kb on - strand, within Psyr_0986 at 1117.449 kb on + strand at 1117.450 kb on - strand at 1117.767 kb on - strand at 1117.797 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 1,115,566 - -2.9 1,115,580 - -0.1 1,115,606 - -0.3 1,115,632 - +0.0 1,115,644 + +2.5 1,115,645 - +0.5 1,115,645 - -0.7 1,115,645 - +0.1 1,115,645 - -0.2 1,115,645 - +2.6 1,115,645 - +0.6 1,115,674 + -0.3 1,115,674 + -0.5 1,115,674 + +2.6 1,115,675 - -1.1 1,115,830 + Psyr_0985 0.32 -0.1 1,115,831 - Psyr_0985 0.32 -0.3 1,115,831 - Psyr_0985 0.32 +0.0 1,115,831 - Psyr_0985 0.32 -0.3 1,115,831 - Psyr_0985 0.32 -1.4 1,115,831 - Psyr_0985 0.32 -1.4 1,115,831 - Psyr_0985 0.32 -0.4 1,115,836 + Psyr_0985 0.33 -0.3 1,115,836 + Psyr_0985 0.33 -2.4 1,115,836 + Psyr_0985 0.33 -0.6 1,115,837 - Psyr_0985 0.33 -2.1 1,115,837 - Psyr_0985 0.33 -0.2 1,116,040 + Psyr_0985 0.68 +0.1 1,116,040 + Psyr_0985 0.68 +0.3 1,116,210 + -1.0 1,116,211 - -0.1 1,116,211 - -0.3 1,116,258 + +0.4 1,116,267 + -1.3 1,116,267 + -0.8 1,116,268 - -0.5 1,116,268 - -0.0 1,116,268 - -1.1 1,116,268 - +0.3 1,116,268 - +0.0 1,116,303 + +0.5 1,116,303 + -0.7 1,116,303 + -0.6 1,116,304 - +0.0 1,116,304 - -0.6 1,116,304 - -0.1 1,116,304 - +1.0 1,116,352 + -1.3 1,116,352 + -0.5 1,116,352 + -0.4 1,116,352 + +2.2 1,116,352 + -0.6 1,116,352 + -0.4 1,116,352 + +0.1 1,116,353 - +0.7 1,116,353 - +0.5 1,116,353 - +2.4 1,116,353 - +0.1 1,116,353 - +0.6 1,116,373 + +0.6 1,116,373 + -0.7 1,116,373 + +0.1 1,116,373 + +1.9 1,116,373 + -1.7 1,116,373 + -0.7 1,116,373 + +1.6 1,116,373 + +0.5 1,116,373 + +0.5 1,116,373 + +0.5 1,116,373 + -1.0 1,116,374 - +0.5 1,116,374 - -1.2 1,116,374 - -1.0 1,116,374 - -0.4 1,116,374 - +0.7 1,116,374 - -0.5 1,116,374 - -0.6 1,116,374 - +2.2 1,116,374 - -0.6 1,116,374 - -0.3 1,116,374 - -1.3 1,116,374 - -1.4 1,116,374 - -0.1 1,116,382 - -0.2 1,116,382 - +1.3 1,116,382 - +0.8 1,116,382 - +0.4 1,116,382 - -1.0 1,116,415 + -1.2 1,116,415 + -0.5 1,116,416 - -0.2 1,116,470 + -1.4 1,116,470 + -0.3 1,116,470 + +0.2 1,116,471 - -1.8 1,116,493 + +1.6 1,116,494 - +0.5 1,116,494 - -0.7 1,116,494 - +0.0 1,116,576 - Psyr_0986 0.10 -0.1 1,116,652 + Psyr_0986 0.18 +0.3 1,116,652 + Psyr_0986 0.18 -1.0 1,116,653 - Psyr_0986 0.18 -0.8 1,116,653 - Psyr_0986 0.18 -0.4 1,116,823 + Psyr_0986 0.35 -2.3 1,117,223 - Psyr_0986 0.75 +0.1 1,117,449 + -1.5 1,117,450 - +0.6 1,117,767 - +1.6 1,117,797 - -0.8
Or see this region's nucleotide sequence