Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0742 and Psyr_0743 are separated by 439 nucleotides Psyr_0743 and Psyr_0744 are separated by 75 nucleotides Psyr_0744 and Psyr_0745 overlap by 124 nucleotides Psyr_0745 and Psyr_0746 are separated by 61 nucleotides Psyr_0746 and Psyr_0747 are separated by 25 nucleotides
Psyr_0742: Psyr_0742 - Enoyl-CoA hydratase/isomerase, at 845,696 to 846,529
_0742
Psyr_0743: Psyr_0743 - CDS, at 846,969 to 847,376
_0743
Psyr_0744: Psyr_0744 - CDS, at 847,452 to 848,153
_0744
Psyr_0745: Psyr_0745 - CDS, at 848,030 to 848,569
_0745
Psyr_0746: Psyr_0746 - IS66 Orf2 like protein, at 848,631 to 848,996
_0746
Psyr_0747: Psyr_0747 - CDS, at 849,022 to 849,339
_0747
Position (kb)
847
848
849 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 846.481 kb on - strand at 846.518 kb on + strand at 846.543 kb on + strand at 846.545 kb on + strand at 846.545 kb on + strand at 846.545 kb on + strand at 846.545 kb on + strand at 846.545 kb on + strand at 846.545 kb on + strand at 846.545 kb on + strand at 846.545 kb on + strand at 846.545 kb on + strand at 846.546 kb on - strand at 846.546 kb on - strand at 846.546 kb on - strand at 846.546 kb on - strand at 846.546 kb on - strand at 846.558 kb on - strand at 846.566 kb on + strand at 846.593 kb on + strand at 846.593 kb on + strand at 846.593 kb on + strand at 846.593 kb on + strand at 846.593 kb on + strand at 846.593 kb on + strand at 846.593 kb on + strand at 846.593 kb on + strand at 846.594 kb on - strand at 846.594 kb on - strand at 846.594 kb on - strand at 846.594 kb on - strand at 846.594 kb on - strand at 846.594 kb on - strand at 846.594 kb on - strand at 846.594 kb on - strand at 846.594 kb on - strand at 846.595 kb on + strand at 846.595 kb on + strand at 846.595 kb on + strand at 846.596 kb on - strand at 846.659 kb on + strand at 846.741 kb on - strand at 846.754 kb on + strand at 846.754 kb on + strand at 846.754 kb on + strand at 846.754 kb on + strand at 846.754 kb on + strand at 846.754 kb on + strand at 846.754 kb on + strand at 846.754 kb on + strand at 846.754 kb on + strand at 846.754 kb on + strand at 846.754 kb on + strand at 846.755 kb on - strand at 846.755 kb on - strand at 846.755 kb on - strand at 846.755 kb on - strand at 846.755 kb on - strand at 846.755 kb on - strand at 846.755 kb on - strand at 846.755 kb on - strand at 846.755 kb on - strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.756 kb on + strand at 846.757 kb on - strand at 846.757 kb on - strand at 846.757 kb on - strand at 846.757 kb on - strand at 846.757 kb on - strand at 846.757 kb on - strand at 846.757 kb on - strand at 846.757 kb on - strand at 846.757 kb on - strand at 846.757 kb on - strand at 846.757 kb on - strand at 846.757 kb on - strand at 846.757 kb on - strand at 846.759 kb on + strand at 846.760 kb on - strand at 846.760 kb on - strand at 846.760 kb on - strand at 846.774 kb on + strand at 846.774 kb on + strand at 846.774 kb on + strand at 846.775 kb on - strand at 846.775 kb on - strand at 846.775 kb on - strand at 846.933 kb on - strand at 846.981 kb on + strand at 846.981 kb on + strand at 846.981 kb on + strand at 846.981 kb on + strand at 846.981 kb on + strand at 846.982 kb on - strand at 846.982 kb on - strand at 846.982 kb on - strand at 846.982 kb on - strand at 846.982 kb on - strand at 846.994 kb on + strand at 846.994 kb on + strand at 846.994 kb on + strand at 846.994 kb on + strand at 846.994 kb on + strand at 846.994 kb on + strand at 846.994 kb on + strand at 846.995 kb on - strand at 846.995 kb on - strand at 847.232 kb on + strand, within Psyr_0743 at 847.232 kb on + strand, within Psyr_0743 at 847.232 kb on + strand, within Psyr_0743 at 847.232 kb on + strand, within Psyr_0743 at 847.233 kb on - strand, within Psyr_0743 at 847.233 kb on - strand, within Psyr_0743 at 847.233 kb on - strand, within Psyr_0743 at 847.233 kb on - strand, within Psyr_0743 at 847.233 kb on - strand, within Psyr_0743 at 847.233 kb on - strand, within Psyr_0743 at 847.235 kb on + strand, within Psyr_0743 at 847.236 kb on - strand, within Psyr_0743 at 847.236 kb on - strand, within Psyr_0743 at 847.236 kb on - strand, within Psyr_0743 at 847.370 kb on + strand at 847.371 kb on - strand at 847.454 kb on - strand at 847.468 kb on + strand at 847.468 kb on + strand at 847.469 kb on - strand at 847.469 kb on - strand at 847.469 kb on - strand at 847.469 kb on - strand at 847.585 kb on + strand, within Psyr_0744 at 847.586 kb on - strand, within Psyr_0744 at 847.613 kb on + strand, within Psyr_0744 at 847.613 kb on + strand, within Psyr_0744 at 847.675 kb on + strand, within Psyr_0744 at 847.675 kb on + strand, within Psyr_0744 at 847.675 kb on + strand, within Psyr_0744 at 847.726 kb on + strand, within Psyr_0744 at 847.726 kb on + strand, within Psyr_0744 at 847.726 kb on + strand, within Psyr_0744 at 847.726 kb on + strand, within Psyr_0744 at 847.727 kb on - strand, within Psyr_0744 at 847.727 kb on - strand, within Psyr_0744 at 847.727 kb on - strand, within Psyr_0744 at 847.727 kb on - strand, within Psyr_0744 at 847.727 kb on - strand, within Psyr_0744 at 847.809 kb on + strand, within Psyr_0744 at 847.810 kb on - strand, within Psyr_0744 at 847.810 kb on - strand, within Psyr_0744 at 847.810 kb on - strand, within Psyr_0744 at 847.810 kb on - strand, within Psyr_0744 at 848.084 kb on + strand at 848.084 kb on + strand at 848.084 kb on + strand at 848.084 kb on + strand at 848.088 kb on + strand at 848.088 kb on + strand at 848.088 kb on + strand at 848.101 kb on + strand at 848.102 kb on - strand at 848.133 kb on - strand at 848.153 kb on + strand at 848.153 kb on + strand at 848.153 kb on + strand at 848.153 kb on + strand at 848.153 kb on + strand at 848.153 kb on + strand at 848.153 kb on + strand at 848.153 kb on + strand at 848.153 kb on + strand at 848.153 kb on + strand at 848.153 kb on + strand at 848.154 kb on - strand, within Psyr_0745 at 848.154 kb on - strand, within Psyr_0745 at 848.154 kb on - strand, within Psyr_0745 at 848.154 kb on - strand, within Psyr_0745 at 848.154 kb on - strand, within Psyr_0745 at 848.154 kb on - strand, within Psyr_0745 at 848.154 kb on - strand, within Psyr_0745 at 848.154 kb on - strand, within Psyr_0745 at 848.187 kb on - strand, within Psyr_0745 at 848.501 kb on + strand, within Psyr_0745 at 848.502 kb on - strand, within Psyr_0745 at 848.502 kb on - strand, within Psyr_0745 at 848.502 kb on - strand, within Psyr_0745 at 848.502 kb on - strand, within Psyr_0745 at 848.540 kb on - strand at 848.661 kb on + strand at 848.661 kb on + strand at 848.685 kb on + strand, within Psyr_0746 at 848.686 kb on - strand, within Psyr_0746 at 848.689 kb on - strand, within Psyr_0746 at 848.738 kb on + strand, within Psyr_0746 at 848.738 kb on + strand, within Psyr_0746 at 848.739 kb on - strand, within Psyr_0746 at 848.848 kb on + strand, within Psyr_0746 at 848.849 kb on - strand, within Psyr_0746 at 849.023 kb on + strand at 849.024 kb on - strand at 849.024 kb on - strand at 849.024 kb on - strand at 849.024 kb on - strand at 849.079 kb on + strand, within Psyr_0747 at 849.079 kb on + strand, within Psyr_0747 at 849.079 kb on + strand, within Psyr_0747 at 849.080 kb on - strand, within Psyr_0747
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 846,481 - +0.2 846,518 + -0.8 846,543 + +2.2 846,545 + -1.5 846,545 + -1.2 846,545 + +2.7 846,545 + -3.5 846,545 + -0.8 846,545 + +1.0 846,545 + -0.8 846,545 + -0.6 846,545 + +1.7 846,546 - +3.0 846,546 - +0.4 846,546 - +0.7 846,546 - +0.1 846,546 - +0.7 846,558 - +1.3 846,566 + -1.6 846,593 + -0.7 846,593 + -1.0 846,593 + +0.9 846,593 + -0.1 846,593 + +0.7 846,593 + -1.1 846,593 + -0.1 846,593 + +1.9 846,594 - +0.1 846,594 - -0.8 846,594 - -0.7 846,594 - -2.5 846,594 - +0.8 846,594 - +1.1 846,594 - +0.2 846,594 - -1.1 846,594 - -1.1 846,595 + +0.0 846,595 + +0.5 846,595 + -0.2 846,596 - +0.9 846,659 + -1.1 846,741 - +0.3 846,754 + -0.9 846,754 + +0.8 846,754 + +4.0 846,754 + -1.5 846,754 + -1.1 846,754 + +0.7 846,754 + +1.4 846,754 + +0.6 846,754 + -0.7 846,754 + -1.0 846,754 + -1.3 846,755 - +1.4 846,755 - -0.1 846,755 - +0.1 846,755 - +0.2 846,755 - +1.3 846,755 - -0.2 846,755 - -0.1 846,755 - -0.8 846,755 - +2.7 846,756 + -1.3 846,756 + +1.4 846,756 + -0.7 846,756 + +0.0 846,756 + +2.1 846,756 + -1.0 846,756 + +0.5 846,756 + +0.0 846,756 + -1.2 846,756 + -0.9 846,756 + -1.2 846,756 + +3.5 846,756 + -0.4 846,756 + +0.4 846,756 + -1.5 846,756 + -1.0 846,757 - +0.4 846,757 - +0.9 846,757 - -1.1 846,757 - +0.3 846,757 - +0.7 846,757 - +0.2 846,757 - +0.2 846,757 - -0.6 846,757 - -0.4 846,757 - -2.2 846,757 - -0.8 846,757 - -1.6 846,757 - -1.0 846,759 + -0.2 846,760 - -1.5 846,760 - +0.9 846,760 - -1.7 846,774 + +0.0 846,774 + +0.1 846,774 + -0.0 846,775 - +0.1 846,775 - -0.8 846,775 - +0.0 846,933 - -0.4 846,981 + +0.8 846,981 + -0.1 846,981 + +0.9 846,981 + +2.0 846,981 + -0.5 846,982 - +1.1 846,982 - -0.0 846,982 - +1.0 846,982 - -0.4 846,982 - -0.3 846,994 + +0.7 846,994 + -0.3 846,994 + +3.1 846,994 + +0.9 846,994 + +3.2 846,994 + +0.1 846,994 + -2.1 846,995 - +3.2 846,995 - -0.8 847,232 + Psyr_0743 0.64 +1.0 847,232 + Psyr_0743 0.64 +0.7 847,232 + Psyr_0743 0.64 +0.1 847,232 + Psyr_0743 0.64 -1.3 847,233 - Psyr_0743 0.65 +1.7 847,233 - Psyr_0743 0.65 -0.3 847,233 - Psyr_0743 0.65 -0.0 847,233 - Psyr_0743 0.65 -1.8 847,233 - Psyr_0743 0.65 +0.3 847,233 - Psyr_0743 0.65 +0.7 847,235 + Psyr_0743 0.65 -0.3 847,236 - Psyr_0743 0.65 -1.1 847,236 - Psyr_0743 0.65 -1.3 847,236 - Psyr_0743 0.65 -0.8 847,370 + -0.1 847,371 - -0.8 847,454 - -1.7 847,468 + +1.1 847,468 + +2.7 847,469 - +0.9 847,469 - +1.2 847,469 - +1.7 847,469 - +1.7 847,585 + Psyr_0744 0.19 -0.6 847,586 - Psyr_0744 0.19 -0.3 847,613 + Psyr_0744 0.23 -0.5 847,613 + Psyr_0744 0.23 -0.1 847,675 + Psyr_0744 0.32 -2.2 847,675 + Psyr_0744 0.32 -2.2 847,675 + Psyr_0744 0.32 +1.2 847,726 + Psyr_0744 0.39 -0.7 847,726 + Psyr_0744 0.39 +1.7 847,726 + Psyr_0744 0.39 -0.4 847,726 + Psyr_0744 0.39 -1.1 847,727 - Psyr_0744 0.39 -0.1 847,727 - Psyr_0744 0.39 -2.1 847,727 - Psyr_0744 0.39 +0.0 847,727 - Psyr_0744 0.39 -0.1 847,727 - Psyr_0744 0.39 +1.4 847,809 + Psyr_0744 0.51 -0.0 847,810 - Psyr_0744 0.51 -1.7 847,810 - Psyr_0744 0.51 -0.4 847,810 - Psyr_0744 0.51 -1.3 847,810 - Psyr_0744 0.51 +0.9 848,084 + -0.8 848,084 + -0.5 848,084 + -1.1 848,084 + -0.7 848,088 + +0.5 848,088 + +0.5 848,088 + -0.1 848,101 + -0.2 848,102 - +0.3 848,133 - -0.7 848,153 + +0.9 848,153 + -1.2 848,153 + +0.5 848,153 + +0.1 848,153 + +1.8 848,153 + -0.8 848,153 + -1.0 848,153 + +0.0 848,153 + +0.8 848,153 + +0.9 848,153 + +2.0 848,154 - Psyr_0745 0.23 +2.3 848,154 - Psyr_0745 0.23 +0.5 848,154 - Psyr_0745 0.23 -1.4 848,154 - Psyr_0745 0.23 +1.1 848,154 - Psyr_0745 0.23 -0.7 848,154 - Psyr_0745 0.23 +0.5 848,154 - Psyr_0745 0.23 -0.3 848,154 - Psyr_0745 0.23 -2.2 848,187 - Psyr_0745 0.29 +0.5 848,501 + Psyr_0745 0.87 -0.0 848,502 - Psyr_0745 0.87 -1.0 848,502 - Psyr_0745 0.87 -0.2 848,502 - Psyr_0745 0.87 -0.9 848,502 - Psyr_0745 0.87 -2.7 848,540 - -0.8 848,661 + +0.1 848,661 + +0.4 848,685 + Psyr_0746 0.15 +0.3 848,686 - Psyr_0746 0.15 +0.2 848,689 - Psyr_0746 0.16 -0.1 848,738 + Psyr_0746 0.29 -0.2 848,738 + Psyr_0746 0.29 +0.7 848,739 - Psyr_0746 0.30 +0.6 848,848 + Psyr_0746 0.59 -1.4 848,849 - Psyr_0746 0.60 +0.5 849,023 + -0.9 849,024 - -0.8 849,024 - -0.7 849,024 - -0.7 849,024 - +0.7 849,079 + Psyr_0747 0.18 -0.3 849,079 + Psyr_0747 0.18 -0.7 849,079 + Psyr_0747 0.18 +0.3 849,080 - Psyr_0747 0.18 +0.3
Or see this region's nucleotide sequence