Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0542 and Psyr_0543 are separated by 226 nucleotides Psyr_0543 and Psyr_0544 are separated by 426 nucleotides
Psyr_0542: Psyr_0542 - Three-deoxy-D-manno-octulosonic-acid transferase, N-terminal, at 611,935 to 613,080
_0542
Psyr_0543: Psyr_0543 - Type I secretion outer membrane protein, TolC, at 613,307 to 614,746
_0543
Psyr_0544: Psyr_0544 - hydroxymethylpyrimidine synthase, at 615,173 to 617,062
_0544
Position (kb)
613
614
615 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 613.137 kb on - strand at 613.386 kb on + strand at 613.386 kb on + strand at 613.476 kb on + strand, within Psyr_0543 at 613.476 kb on + strand, within Psyr_0543 at 613.476 kb on + strand, within Psyr_0543 at 613.476 kb on + strand, within Psyr_0543 at 613.476 kb on + strand, within Psyr_0543 at 613.477 kb on - strand, within Psyr_0543 at 613.477 kb on - strand, within Psyr_0543 at 613.477 kb on - strand, within Psyr_0543 at 613.483 kb on - strand, within Psyr_0543 at 613.497 kb on + strand, within Psyr_0543 at 613.497 kb on + strand, within Psyr_0543 at 613.498 kb on - strand, within Psyr_0543 at 613.498 kb on - strand, within Psyr_0543 at 613.515 kb on + strand, within Psyr_0543 at 613.515 kb on + strand, within Psyr_0543 at 613.515 kb on + strand, within Psyr_0543 at 613.515 kb on + strand, within Psyr_0543 at 613.516 kb on - strand, within Psyr_0543 at 613.516 kb on - strand, within Psyr_0543 at 613.648 kb on + strand, within Psyr_0543 at 613.737 kb on + strand, within Psyr_0543 at 613.776 kb on + strand, within Psyr_0543 at 613.776 kb on + strand, within Psyr_0543 at 613.777 kb on - strand, within Psyr_0543 at 613.845 kb on + strand, within Psyr_0543 at 613.845 kb on + strand, within Psyr_0543 at 613.845 kb on + strand, within Psyr_0543 at 613.846 kb on - strand, within Psyr_0543 at 613.890 kb on + strand, within Psyr_0543 at 613.890 kb on + strand, within Psyr_0543 at 613.890 kb on + strand, within Psyr_0543 at 613.890 kb on + strand, within Psyr_0543 at 613.890 kb on + strand, within Psyr_0543 at 613.891 kb on - strand, within Psyr_0543 at 613.891 kb on - strand, within Psyr_0543 at 613.968 kb on + strand, within Psyr_0543 at 613.969 kb on - strand, within Psyr_0543 at 614.060 kb on - strand, within Psyr_0543 at 614.085 kb on + strand, within Psyr_0543 at 614.085 kb on + strand, within Psyr_0543 at 614.085 kb on + strand, within Psyr_0543 at 614.086 kb on - strand, within Psyr_0543 at 614.172 kb on + strand, within Psyr_0543 at 614.172 kb on + strand, within Psyr_0543 at 614.173 kb on - strand, within Psyr_0543 at 614.173 kb on - strand, within Psyr_0543 at 614.306 kb on + strand, within Psyr_0543 at 614.306 kb on + strand, within Psyr_0543 at 614.316 kb on + strand, within Psyr_0543 at 614.316 kb on + strand, within Psyr_0543 at 614.316 kb on + strand, within Psyr_0543 at 614.317 kb on - strand, within Psyr_0543 at 614.317 kb on - strand, within Psyr_0543 at 614.317 kb on - strand, within Psyr_0543 at 614.589 kb on + strand, within Psyr_0543 at 614.589 kb on + strand, within Psyr_0543 at 614.774 kb on - strand at 614.777 kb on + strand at 614.777 kb on + strand at 614.778 kb on - strand at 614.814 kb on + strand at 614.814 kb on + strand at 614.814 kb on + strand at 614.815 kb on - strand at 614.815 kb on - strand at 614.815 kb on - strand at 614.849 kb on - strand at 614.857 kb on + strand at 614.857 kb on + strand at 614.876 kb on - strand at 614.876 kb on - strand at 614.876 kb on - strand at 614.904 kb on + strand at 614.904 kb on + strand at 614.904 kb on + strand at 614.905 kb on - strand at 614.905 kb on - strand at 614.905 kb on - strand at 614.905 kb on - strand at 614.937 kb on + strand at 614.938 kb on - strand at 614.938 kb on - strand at 614.938 kb on - strand at 614.938 kb on - strand at 615.062 kb on + strand at 615.063 kb on - strand at 615.141 kb on + strand at 615.141 kb on + strand at 615.141 kb on + strand at 615.142 kb on - strand at 615.178 kb on + strand at 615.178 kb on + strand at 615.178 kb on + strand at 615.178 kb on + strand at 615.178 kb on + strand at 615.178 kb on + strand at 615.180 kb on + strand at 615.180 kb on + strand at 615.180 kb on + strand at 615.180 kb on + strand at 615.181 kb on - strand at 615.181 kb on - strand at 615.181 kb on - strand at 615.181 kb on - strand at 615.181 kb on - strand at 615.181 kb on - strand at 615.266 kb on + strand at 615.267 kb on - strand at 615.371 kb on + strand, within Psyr_0544 at 615.371 kb on + strand, within Psyr_0544 at 615.372 kb on - strand, within Psyr_0544 at 615.389 kb on + strand, within Psyr_0544 at 615.605 kb on + strand, within Psyr_0544 at 615.606 kb on - strand, within Psyr_0544 at 615.606 kb on - strand, within Psyr_0544 at 615.641 kb on + strand, within Psyr_0544 at 615.641 kb on + strand, within Psyr_0544 at 615.641 kb on + strand, within Psyr_0544 at 615.641 kb on + strand, within Psyr_0544 at 615.641 kb on + strand, within Psyr_0544 at 615.641 kb on + strand, within Psyr_0544 at 615.642 kb on - strand, within Psyr_0544 at 615.733 kb on + strand, within Psyr_0544 at 615.734 kb on - strand, within Psyr_0544
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 613,137 - +0.0 613,386 + -0.8 613,386 + -0.9 613,476 + Psyr_0543 0.12 +0.5 613,476 + Psyr_0543 0.12 -0.2 613,476 + Psyr_0543 0.12 -0.2 613,476 + Psyr_0543 0.12 +0.1 613,476 + Psyr_0543 0.12 -3.1 613,477 - Psyr_0543 0.12 -2.0 613,477 - Psyr_0543 0.12 +1.6 613,477 - Psyr_0543 0.12 -0.6 613,483 - Psyr_0543 0.12 +0.4 613,497 + Psyr_0543 0.13 +0.2 613,497 + Psyr_0543 0.13 +1.0 613,498 - Psyr_0543 0.13 -0.7 613,498 - Psyr_0543 0.13 +1.5 613,515 + Psyr_0543 0.14 -0.7 613,515 + Psyr_0543 0.14 -0.1 613,515 + Psyr_0543 0.14 -1.0 613,515 + Psyr_0543 0.14 -1.5 613,516 - Psyr_0543 0.15 -0.2 613,516 - Psyr_0543 0.15 -0.7 613,648 + Psyr_0543 0.24 -0.3 613,737 + Psyr_0543 0.30 +0.0 613,776 + Psyr_0543 0.33 -1.5 613,776 + Psyr_0543 0.33 +3.4 613,777 - Psyr_0543 0.33 +0.0 613,845 + Psyr_0543 0.37 +0.5 613,845 + Psyr_0543 0.37 +0.3 613,845 + Psyr_0543 0.37 -1.0 613,846 - Psyr_0543 0.37 -0.7 613,890 + Psyr_0543 0.40 -0.0 613,890 + Psyr_0543 0.40 +0.6 613,890 + Psyr_0543 0.40 -0.9 613,890 + Psyr_0543 0.40 +0.2 613,890 + Psyr_0543 0.40 -2.5 613,891 - Psyr_0543 0.41 -0.4 613,891 - Psyr_0543 0.41 -1.1 613,968 + Psyr_0543 0.46 -0.3 613,969 - Psyr_0543 0.46 +0.7 614,060 - Psyr_0543 0.52 +0.8 614,085 + Psyr_0543 0.54 +0.8 614,085 + Psyr_0543 0.54 +0.1 614,085 + Psyr_0543 0.54 -2.6 614,086 - Psyr_0543 0.54 -1.2 614,172 + Psyr_0543 0.60 +0.7 614,172 + Psyr_0543 0.60 +0.5 614,173 - Psyr_0543 0.60 -0.2 614,173 - Psyr_0543 0.60 -1.2 614,306 + Psyr_0543 0.69 -1.3 614,306 + Psyr_0543 0.69 +1.2 614,316 + Psyr_0543 0.70 -0.6 614,316 + Psyr_0543 0.70 -2.2 614,316 + Psyr_0543 0.70 -1.9 614,317 - Psyr_0543 0.70 -1.3 614,317 - Psyr_0543 0.70 -0.1 614,317 - Psyr_0543 0.70 +1.1 614,589 + Psyr_0543 0.89 +0.4 614,589 + Psyr_0543 0.89 -1.0 614,774 - +0.2 614,777 + +0.6 614,777 + -0.2 614,778 - +1.1 614,814 + +0.0 614,814 + +1.2 614,814 + +0.4 614,815 - -1.4 614,815 - -2.0 614,815 - -0.8 614,849 - +0.5 614,857 + -1.6 614,857 + -0.6 614,876 - -0.9 614,876 - -0.8 614,876 - -1.2 614,904 + +1.0 614,904 + -0.5 614,904 + +1.4 614,905 - -0.7 614,905 - +0.0 614,905 - +1.8 614,905 - +1.8 614,937 + +0.3 614,938 - +0.2 614,938 - -1.2 614,938 - -0.3 614,938 - -1.7 615,062 + +0.2 615,063 - -1.0 615,141 + +0.1 615,141 + -1.5 615,141 + +0.7 615,142 - +0.3 615,178 + -0.5 615,178 + +1.1 615,178 + +0.5 615,178 + -0.3 615,178 + -1.6 615,178 + +0.8 615,180 + +0.3 615,180 + -0.5 615,180 + +0.3 615,180 + +0.6 615,181 - +3.4 615,181 - +1.8 615,181 - +0.9 615,181 - -1.7 615,181 - +0.4 615,181 - +0.5 615,266 + +0.4 615,267 - +0.2 615,371 + Psyr_0544 0.10 -0.7 615,371 + Psyr_0544 0.10 -1.7 615,372 - Psyr_0544 0.11 -0.4 615,389 + Psyr_0544 0.11 +0.8 615,605 + Psyr_0544 0.23 +0.2 615,606 - Psyr_0544 0.23 -0.0 615,606 - Psyr_0544 0.23 -0.1 615,641 + Psyr_0544 0.25 +1.2 615,641 + Psyr_0544 0.25 +0.6 615,641 + Psyr_0544 0.25 +1.0 615,641 + Psyr_0544 0.25 +1.1 615,641 + Psyr_0544 0.25 -0.7 615,641 + Psyr_0544 0.25 +0.1 615,642 - Psyr_0544 0.25 +0.1 615,733 + Psyr_0544 0.30 -0.3 615,734 - Psyr_0544 0.30 +0.4
Or see this region's nucleotide sequence