Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0245 and Psyr_0246 are separated by 153 nucleotides Psyr_0246 and Psyr_0247 are separated by 43 nucleotides Psyr_0247 and Psyr_0248 are separated by 69 nucleotides Psyr_0248 and Psyr_0249 are separated by 141 nucleotides
Psyr_0245: Psyr_0245 - Binding-protein-dependent transport systems inner membrane component, at 257,895 to 259,511
_0245
Psyr_0246: Psyr_0246 - aminomethyltransferase, at 259,665 to 260,747
_0246
Psyr_0247: Psyr_0247 - glycine cleavage system H protein, at 260,791 to 261,177
_0247
Psyr_0248: Psyr_0248 - conserved hypothetical protein, at 261,247 to 261,567
_0248
Psyr_0249: Psyr_0249 - type II secretion system protein E:General secretory system II, protein E, N-terminal, at 261,709 to 263,493
_0249
Position (kb)
259
260
261 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 258.876 kb on + strand, within Psyr_0245 at 258.876 kb on + strand, within Psyr_0245 at 258.877 kb on - strand, within Psyr_0245 at 258.941 kb on + strand, within Psyr_0245 at 258.941 kb on + strand, within Psyr_0245 at 258.941 kb on + strand, within Psyr_0245 at 258.941 kb on + strand, within Psyr_0245 at 258.942 kb on - strand, within Psyr_0245 at 258.942 kb on - strand, within Psyr_0245 at 258.942 kb on - strand, within Psyr_0245 at 258.942 kb on - strand, within Psyr_0245 at 258.942 kb on - strand, within Psyr_0245 at 259.044 kb on + strand, within Psyr_0245 at 259.044 kb on + strand, within Psyr_0245 at 259.135 kb on + strand, within Psyr_0245 at 259.140 kb on + strand, within Psyr_0245 at 259.266 kb on + strand, within Psyr_0245 at 259.266 kb on + strand, within Psyr_0245 at 259.266 kb on + strand, within Psyr_0245 at 259.266 kb on + strand, within Psyr_0245 at 259.266 kb on + strand, within Psyr_0245 at 259.267 kb on - strand, within Psyr_0245 at 259.267 kb on - strand, within Psyr_0245 at 259.267 kb on - strand, within Psyr_0245 at 259.352 kb on + strand at 259.456 kb on + strand at 259.456 kb on + strand at 259.456 kb on + strand at 259.457 kb on - strand at 259.462 kb on + strand at 259.462 kb on + strand at 259.463 kb on - strand at 259.463 kb on - strand at 259.463 kb on - strand at 259.463 kb on - strand at 259.509 kb on + strand at 259.509 kb on + strand at 259.510 kb on - strand at 259.568 kb on + strand at 259.568 kb on + strand at 259.568 kb on + strand at 259.584 kb on + strand at 259.585 kb on - strand at 259.585 kb on - strand at 259.676 kb on + strand at 259.885 kb on + strand, within Psyr_0246 at 259.885 kb on + strand, within Psyr_0246 at 259.885 kb on + strand, within Psyr_0246 at 259.885 kb on + strand, within Psyr_0246 at 259.885 kb on + strand, within Psyr_0246 at 259.885 kb on + strand, within Psyr_0246 at 259.885 kb on + strand, within Psyr_0246 at 259.885 kb on + strand, within Psyr_0246 at 259.886 kb on - strand, within Psyr_0246 at 259.886 kb on - strand, within Psyr_0246 at 259.886 kb on - strand, within Psyr_0246 at 259.886 kb on - strand, within Psyr_0246 at 259.886 kb on - strand, within Psyr_0246 at 259.886 kb on - strand, within Psyr_0246 at 259.886 kb on - strand, within Psyr_0246 at 259.886 kb on - strand, within Psyr_0246 at 259.917 kb on + strand, within Psyr_0246 at 259.917 kb on + strand, within Psyr_0246 at 259.918 kb on - strand, within Psyr_0246 at 259.918 kb on - strand, within Psyr_0246 at 259.918 kb on - strand, within Psyr_0246 at 259.918 kb on - strand, within Psyr_0246 at 259.918 kb on - strand, within Psyr_0246 at 259.918 kb on - strand, within Psyr_0246 at 259.918 kb on - strand, within Psyr_0246 at 259.918 kb on - strand, within Psyr_0246 at 259.968 kb on + strand, within Psyr_0246 at 259.968 kb on + strand, within Psyr_0246 at 260.227 kb on - strand, within Psyr_0246 at 260.368 kb on - strand, within Psyr_0246 at 260.454 kb on - strand, within Psyr_0246 at 260.557 kb on + strand, within Psyr_0246 at 260.557 kb on + strand, within Psyr_0246 at 260.557 kb on + strand, within Psyr_0246 at 260.557 kb on + strand, within Psyr_0246 at 260.557 kb on + strand, within Psyr_0246 at 260.557 kb on + strand, within Psyr_0246 at 260.557 kb on + strand, within Psyr_0246 at 260.558 kb on - strand, within Psyr_0246 at 260.558 kb on - strand, within Psyr_0246 at 260.558 kb on - strand, within Psyr_0246 at 260.558 kb on - strand, within Psyr_0246 at 260.558 kb on - strand, within Psyr_0246 at 260.558 kb on - strand, within Psyr_0246 at 260.558 kb on - strand, within Psyr_0246 at 260.597 kb on + strand, within Psyr_0246 at 260.609 kb on + strand, within Psyr_0246 at 260.609 kb on + strand, within Psyr_0246 at 260.609 kb on + strand, within Psyr_0246 at 260.610 kb on - strand, within Psyr_0246 at 260.610 kb on - strand, within Psyr_0246 at 260.610 kb on - strand, within Psyr_0246 at 260.610 kb on - strand, within Psyr_0246 at 260.745 kb on + strand at 260.745 kb on + strand at 260.746 kb on - strand at 260.750 kb on + strand at 260.750 kb on + strand at 260.750 kb on + strand at 260.750 kb on + strand at 260.788 kb on + strand at 260.788 kb on + strand at 260.788 kb on + strand at 260.788 kb on + strand at 260.788 kb on + strand at 260.789 kb on - strand at 260.789 kb on - strand at 260.789 kb on - strand at 260.799 kb on + strand at 260.998 kb on - strand, within Psyr_0247 at 261.001 kb on + strand, within Psyr_0247 at 261.001 kb on + strand, within Psyr_0247 at 261.001 kb on + strand, within Psyr_0247 at 261.001 kb on + strand, within Psyr_0247 at 261.001 kb on + strand, within Psyr_0247 at 261.001 kb on + strand, within Psyr_0247 at 261.001 kb on + strand, within Psyr_0247 at 261.001 kb on + strand, within Psyr_0247 at 261.002 kb on - strand, within Psyr_0247 at 261.002 kb on - strand, within Psyr_0247 at 261.002 kb on - strand, within Psyr_0247 at 261.002 kb on - strand, within Psyr_0247 at 261.154 kb on + strand at 261.248 kb on + strand at 261.248 kb on + strand at 261.248 kb on + strand at 261.248 kb on + strand at 261.248 kb on + strand at 261.249 kb on - strand at 261.249 kb on - strand at 261.449 kb on + strand, within Psyr_0248 at 261.449 kb on + strand, within Psyr_0248 at 261.449 kb on + strand, within Psyr_0248 at 261.450 kb on - strand, within Psyr_0248 at 261.450 kb on - strand, within Psyr_0248 at 261.575 kb on + strand at 261.575 kb on + strand at 261.576 kb on - strand at 261.576 kb on - strand at 261.576 kb on - strand at 261.611 kb on + strand at 261.611 kb on + strand at 261.611 kb on + strand at 261.611 kb on + strand at 261.612 kb on - strand at 261.612 kb on - strand at 261.612 kb on - strand at 261.612 kb on - strand at 261.624 kb on + strand at 261.624 kb on + strand at 261.624 kb on + strand at 261.624 kb on + strand at 261.624 kb on + strand at 261.624 kb on + strand at 261.624 kb on + strand at 261.625 kb on - strand at 261.625 kb on - strand at 261.625 kb on - strand at 261.625 kb on - strand at 261.625 kb on - strand at 261.625 kb on - strand at 261.625 kb on - strand at 261.657 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 258,876 + Psyr_0245 0.61 -0.5 258,876 + Psyr_0245 0.61 -0.7 258,877 - Psyr_0245 0.61 +0.5 258,941 + Psyr_0245 0.65 +0.0 258,941 + Psyr_0245 0.65 -1.8 258,941 + Psyr_0245 0.65 -0.9 258,941 + Psyr_0245 0.65 +2.6 258,942 - Psyr_0245 0.65 -0.9 258,942 - Psyr_0245 0.65 -1.8 258,942 - Psyr_0245 0.65 +0.9 258,942 - Psyr_0245 0.65 -1.2 258,942 - Psyr_0245 0.65 -0.0 259,044 + Psyr_0245 0.71 +0.3 259,044 + Psyr_0245 0.71 -0.7 259,135 + Psyr_0245 0.77 +0.6 259,140 + Psyr_0245 0.77 -0.7 259,266 + Psyr_0245 0.85 +0.5 259,266 + Psyr_0245 0.85 -1.1 259,266 + Psyr_0245 0.85 +1.5 259,266 + Psyr_0245 0.85 +0.6 259,266 + Psyr_0245 0.85 +1.0 259,267 - Psyr_0245 0.85 +0.2 259,267 - Psyr_0245 0.85 -0.1 259,267 - Psyr_0245 0.85 +0.1 259,352 + -1.4 259,456 + +0.7 259,456 + -0.8 259,456 + -1.1 259,457 - +0.3 259,462 + -0.5 259,462 + -0.3 259,463 - -0.2 259,463 - -0.1 259,463 - -1.4 259,463 - -0.3 259,509 + +0.9 259,509 + -0.8 259,510 - +0.2 259,568 + -1.6 259,568 + -0.6 259,568 + -1.3 259,584 + +0.9 259,585 - -0.1 259,585 - -1.2 259,676 + +0.8 259,885 + Psyr_0246 0.20 -0.1 259,885 + Psyr_0246 0.20 -2.1 259,885 + Psyr_0246 0.20 +1.1 259,885 + Psyr_0246 0.20 -1.5 259,885 + Psyr_0246 0.20 +1.1 259,885 + Psyr_0246 0.20 +0.9 259,885 + Psyr_0246 0.20 -0.9 259,885 + Psyr_0246 0.20 -0.2 259,886 - Psyr_0246 0.20 +0.5 259,886 - Psyr_0246 0.20 -0.4 259,886 - Psyr_0246 0.20 -0.1 259,886 - Psyr_0246 0.20 -1.8 259,886 - Psyr_0246 0.20 -0.6 259,886 - Psyr_0246 0.20 +1.5 259,886 - Psyr_0246 0.20 -0.3 259,886 - Psyr_0246 0.20 -1.7 259,917 + Psyr_0246 0.23 -2.0 259,917 + Psyr_0246 0.23 -0.5 259,918 - Psyr_0246 0.23 -0.1 259,918 - Psyr_0246 0.23 +1.4 259,918 - Psyr_0246 0.23 +0.8 259,918 - Psyr_0246 0.23 +0.6 259,918 - Psyr_0246 0.23 -1.6 259,918 - Psyr_0246 0.23 +0.3 259,918 - Psyr_0246 0.23 -0.7 259,918 - Psyr_0246 0.23 -1.2 259,968 + Psyr_0246 0.28 +0.5 259,968 + Psyr_0246 0.28 -0.3 260,227 - Psyr_0246 0.52 -0.1 260,368 - Psyr_0246 0.65 -1.1 260,454 - Psyr_0246 0.73 -1.4 260,557 + Psyr_0246 0.82 +0.1 260,557 + Psyr_0246 0.82 -1.6 260,557 + Psyr_0246 0.82 -2.0 260,557 + Psyr_0246 0.82 -1.4 260,557 + Psyr_0246 0.82 +1.9 260,557 + Psyr_0246 0.82 -0.6 260,557 + Psyr_0246 0.82 -1.4 260,558 - Psyr_0246 0.82 +1.9 260,558 - Psyr_0246 0.82 +0.3 260,558 - Psyr_0246 0.82 +0.5 260,558 - Psyr_0246 0.82 -0.1 260,558 - Psyr_0246 0.82 +4.7 260,558 - Psyr_0246 0.82 -0.9 260,558 - Psyr_0246 0.82 -0.4 260,597 + Psyr_0246 0.86 -1.0 260,609 + Psyr_0246 0.87 -0.1 260,609 + Psyr_0246 0.87 -1.7 260,609 + Psyr_0246 0.87 -0.3 260,610 - Psyr_0246 0.87 -1.7 260,610 - Psyr_0246 0.87 -2.5 260,610 - Psyr_0246 0.87 +0.2 260,610 - Psyr_0246 0.87 -0.4 260,745 + +0.5 260,745 + +0.8 260,746 - +0.7 260,750 + -0.8 260,750 + +1.3 260,750 + -0.1 260,750 + +0.3 260,788 + +0.7 260,788 + -0.4 260,788 + -0.1 260,788 + +0.3 260,788 + +0.1 260,789 - +1.0 260,789 - +0.8 260,789 - +0.4 260,799 + -1.1 260,998 - Psyr_0247 0.53 +0.6 261,001 + Psyr_0247 0.54 -0.3 261,001 + Psyr_0247 0.54 +1.0 261,001 + Psyr_0247 0.54 -0.8 261,001 + Psyr_0247 0.54 -0.8 261,001 + Psyr_0247 0.54 -0.8 261,001 + Psyr_0247 0.54 -0.6 261,001 + Psyr_0247 0.54 -1.9 261,001 + Psyr_0247 0.54 +0.7 261,002 - Psyr_0247 0.55 +0.1 261,002 - Psyr_0247 0.55 -1.0 261,002 - Psyr_0247 0.55 -0.3 261,002 - Psyr_0247 0.55 -0.9 261,154 + -0.5 261,248 + +1.5 261,248 + -0.1 261,248 + +0.6 261,248 + -0.9 261,248 + +0.5 261,249 - -0.9 261,249 - +0.8 261,449 + Psyr_0248 0.63 +1.2 261,449 + Psyr_0248 0.63 +2.6 261,449 + Psyr_0248 0.63 -1.2 261,450 - Psyr_0248 0.63 +1.2 261,450 - Psyr_0248 0.63 +0.1 261,575 + -0.1 261,575 + -1.3 261,576 - +1.3 261,576 - +0.9 261,576 - +0.9 261,611 + -1.5 261,611 + -0.2 261,611 + -0.0 261,611 + -2.3 261,612 - -0.5 261,612 - -0.6 261,612 - -1.0 261,612 - -1.6 261,624 + +0.2 261,624 + -0.1 261,624 + -1.8 261,624 + +1.9 261,624 + -0.1 261,624 + -0.7 261,624 + +0.9 261,625 - -0.0 261,625 - +0.2 261,625 - +0.1 261,625 - +1.4 261,625 - +0.8 261,625 - +0.3 261,625 - -1.4 261,657 + +1.7
Or see this region's nucleotide sequence