Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0066

Experiment: KB with Avocatin A 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0065 and Psyr_0066 are separated by 405 nucleotidesPsyr_0066 and Psyr_0067 are separated by 4 nucleotides Psyr_0065: Psyr_0065 - argininosuccinate lyase, at 64,605 to 65,999 _0065 Psyr_0066: Psyr_0066 - bifunctional spore maturation protein, fused SpmA/SpmB, at 66,405 to 67,646 _0066 Psyr_0067: Psyr_0067 - membrane protein, putative, at 67,651 to 68,844 _0067 Position (kb) 66 67 68Strain fitness (log2 ratio) -2 -1 0 1 2at 66.044 kb on - strandat 66.143 kb on + strandat 66.143 kb on + strandat 66.144 kb on - strandat 66.144 kb on - strandat 66.144 kb on - strandat 66.144 kb on - strandat 66.315 kb on + strandat 66.315 kb on + strandat 66.315 kb on + strandat 66.315 kb on + strandat 66.315 kb on + strandat 66.315 kb on + strandat 66.316 kb on - strandat 66.316 kb on - strandat 66.316 kb on - strandat 66.316 kb on - strandat 66.316 kb on - strandat 66.316 kb on - strandat 66.316 kb on - strandat 66.316 kb on - strandat 66.316 kb on - strandat 66.324 kb on + strandat 66.324 kb on + strandat 66.324 kb on + strandat 66.325 kb on - strandat 66.325 kb on - strandat 66.325 kb on - strandat 66.325 kb on - strandat 66.325 kb on - strandat 66.399 kb on + strandat 66.399 kb on + strandat 66.399 kb on + strandat 66.399 kb on + strandat 66.400 kb on - strandat 66.513 kb on - strandat 66.513 kb on - strandat 66.574 kb on + strand, within Psyr_0066at 66.575 kb on - strand, within Psyr_0066at 66.575 kb on - strand, within Psyr_0066at 66.575 kb on - strand, within Psyr_0066at 66.883 kb on - strand, within Psyr_0066at 66.883 kb on - strand, within Psyr_0066at 66.883 kb on - strand, within Psyr_0066at 67.023 kb on + strand, within Psyr_0066at 67.023 kb on + strand, within Psyr_0066at 67.024 kb on - strand, within Psyr_0066at 67.024 kb on - strand, within Psyr_0066at 67.169 kb on - strand, within Psyr_0066at 67.245 kb on + strand, within Psyr_0066at 67.246 kb on - strand, within Psyr_0066at 67.246 kb on - strand, within Psyr_0066at 67.506 kb on + strand, within Psyr_0066at 67.506 kb on + strand, within Psyr_0066at 67.506 kb on + strand, within Psyr_0066at 67.506 kb on + strand, within Psyr_0066at 67.506 kb on + strand, within Psyr_0066at 67.506 kb on + strand, within Psyr_0066at 67.506 kb on + strand, within Psyr_0066at 67.506 kb on + strand, within Psyr_0066at 67.507 kb on - strand, within Psyr_0066at 67.507 kb on - strand, within Psyr_0066at 67.507 kb on - strand, within Psyr_0066at 67.507 kb on - strand, within Psyr_0066at 67.507 kb on - strand, within Psyr_0066at 67.507 kb on - strand, within Psyr_0066at 67.507 kb on - strand, within Psyr_0066at 67.510 kb on + strand, within Psyr_0066at 67.510 kb on + strand, within Psyr_0066at 67.510 kb on + strand, within Psyr_0066at 67.511 kb on - strand, within Psyr_0066at 67.511 kb on - strand, within Psyr_0066at 67.511 kb on - strand, within Psyr_0066at 67.511 kb on - strand, within Psyr_0066at 67.522 kb on - strandat 67.652 kb on + strandat 67.755 kb on + strandat 67.756 kb on - strandat 67.862 kb on - strand, within Psyr_0067at 68.024 kb on + strand, within Psyr_0067at 68.024 kb on + strand, within Psyr_0067at 68.024 kb on + strand, within Psyr_0067at 68.024 kb on + strand, within Psyr_0067at 68.024 kb on + strand, within Psyr_0067at 68.024 kb on + strand, within Psyr_0067at 68.025 kb on - strand, within Psyr_0067at 68.025 kb on - strand, within Psyr_0067at 68.025 kb on - strand, within Psyr_0067at 68.025 kb on - strand, within Psyr_0067at 68.025 kb on - strand, within Psyr_0067at 68.025 kb on - strand, within Psyr_0067at 68.025 kb on - strand, within Psyr_0067at 68.025 kb on - strand, within Psyr_0067at 68.025 kb on - strand, within Psyr_0067at 68.025 kb on - strand, within Psyr_0067at 68.025 kb on - strand, within Psyr_0067at 68.028 kb on + strand, within Psyr_0067at 68.028 kb on + strand, within Psyr_0067at 68.028 kb on + strand, within Psyr_0067at 68.028 kb on + strand, within Psyr_0067at 68.028 kb on + strand, within Psyr_0067at 68.028 kb on + strand, within Psyr_0067at 68.028 kb on + strand, within Psyr_0067at 68.029 kb on - strand, within Psyr_0067at 68.029 kb on - strand, within Psyr_0067at 68.029 kb on - strand, within Psyr_0067at 68.066 kb on + strand, within Psyr_0067at 68.066 kb on + strand, within Psyr_0067at 68.067 kb on - strand, within Psyr_0067at 68.072 kb on + strand, within Psyr_0067at 68.072 kb on + strand, within Psyr_0067at 68.072 kb on + strand, within Psyr_0067at 68.073 kb on - strand, within Psyr_0067at 68.073 kb on - strand, within Psyr_0067at 68.115 kb on + strand, within Psyr_0067at 68.115 kb on + strand, within Psyr_0067at 68.116 kb on - strand, within Psyr_0067at 68.116 kb on - strand, within Psyr_0067at 68.311 kb on + strand, within Psyr_0067at 68.312 kb on - strand, within Psyr_0067at 68.339 kb on + strand, within Psyr_0067at 68.339 kb on + strand, within Psyr_0067at 68.340 kb on - strand, within Psyr_0067at 68.453 kb on + strand, within Psyr_0067at 68.453 kb on + strand, within Psyr_0067at 68.453 kb on + strand, within Psyr_0067at 68.453 kb on + strand, within Psyr_0067at 68.454 kb on - strand, within Psyr_0067at 68.454 kb on - strand, within Psyr_0067at 68.454 kb on - strand, within Psyr_0067at 68.454 kb on - strand, within Psyr_0067at 68.456 kb on + strand, within Psyr_0067at 68.456 kb on + strand, within Psyr_0067at 68.456 kb on + strand, within Psyr_0067at 68.456 kb on + strand, within Psyr_0067at 68.456 kb on + strand, within Psyr_0067at 68.457 kb on - strand, within Psyr_0067at 68.457 kb on - strand, within Psyr_0067at 68.457 kb on - strand, within Psyr_0067

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM
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66,044 - -0.1
66,143 + -0.6
66,143 + +1.8
66,144 - -1.3
66,144 - -0.4
66,144 - +2.0
66,144 - -0.2
66,315 + -0.6
66,315 + -1.0
66,315 + +0.4
66,315 + -1.8
66,315 + -0.8
66,315 + -1.4
66,316 - +0.8
66,316 - -0.3
66,316 - -0.2
66,316 - +1.0
66,316 - +0.5
66,316 - +0.5
66,316 - -0.8
66,316 - +0.9
66,316 - -0.8
66,324 + +0.9
66,324 + -0.6
66,324 + -0.1
66,325 - -0.7
66,325 - -0.5
66,325 - -0.1
66,325 - +1.5
66,325 - -0.1
66,399 + -1.0
66,399 + -2.6
66,399 + +0.0
66,399 + +1.5
66,400 - -1.4
66,513 - +0.1
66,513 - +0.4
66,574 + Psyr_0066 0.14 -1.6
66,575 - Psyr_0066 0.14 +0.7
66,575 - Psyr_0066 0.14 -1.7
66,575 - Psyr_0066 0.14 +0.3
66,883 - Psyr_0066 0.38 +0.6
66,883 - Psyr_0066 0.38 -1.3
66,883 - Psyr_0066 0.38 +0.4
67,023 + Psyr_0066 0.50 +0.0
67,023 + Psyr_0066 0.50 +0.5
67,024 - Psyr_0066 0.50 +0.2
67,024 - Psyr_0066 0.50 +0.6
67,169 - Psyr_0066 0.62 +0.7
67,245 + Psyr_0066 0.68 -1.5
67,246 - Psyr_0066 0.68 -0.6
67,246 - Psyr_0066 0.68 +2.6
67,506 + Psyr_0066 0.89 -0.8
67,506 + Psyr_0066 0.89 -0.9
67,506 + Psyr_0066 0.89 -0.4
67,506 + Psyr_0066 0.89 +0.1
67,506 + Psyr_0066 0.89 -1.0
67,506 + Psyr_0066 0.89 +1.7
67,506 + Psyr_0066 0.89 +2.0
67,506 + Psyr_0066 0.89 -1.0
67,507 - Psyr_0066 0.89 +1.0
67,507 - Psyr_0066 0.89 +1.0
67,507 - Psyr_0066 0.89 -1.4
67,507 - Psyr_0066 0.89 +0.7
67,507 - Psyr_0066 0.89 -0.0
67,507 - Psyr_0066 0.89 -0.6
67,507 - Psyr_0066 0.89 -1.4
67,510 + Psyr_0066 0.89 +1.0
67,510 + Psyr_0066 0.89 -2.1
67,510 + Psyr_0066 0.89 -0.3
67,511 - Psyr_0066 0.89 -2.1
67,511 - Psyr_0066 0.89 -0.2
67,511 - Psyr_0066 0.89 +1.6
67,511 - Psyr_0066 0.89 -1.6
67,522 - +1.3
67,652 + -0.8
67,755 + +0.8
67,756 - -0.2
67,862 - Psyr_0067 0.18 +2.0
68,024 + Psyr_0067 0.31 -0.5
68,024 + Psyr_0067 0.31 +0.6
68,024 + Psyr_0067 0.31 +1.0
68,024 + Psyr_0067 0.31 -0.9
68,024 + Psyr_0067 0.31 +0.8
68,024 + Psyr_0067 0.31 +0.1
68,025 - Psyr_0067 0.31 -0.5
68,025 - Psyr_0067 0.31 -0.9
68,025 - Psyr_0067 0.31 -0.9
68,025 - Psyr_0067 0.31 -1.0
68,025 - Psyr_0067 0.31 -0.0
68,025 - Psyr_0067 0.31 -1.2
68,025 - Psyr_0067 0.31 -1.5
68,025 - Psyr_0067 0.31 -0.2
68,025 - Psyr_0067 0.31 -0.1
68,025 - Psyr_0067 0.31 -0.2
68,025 - Psyr_0067 0.31 -0.5
68,028 + Psyr_0067 0.32 -1.9
68,028 + Psyr_0067 0.32 -1.3
68,028 + Psyr_0067 0.32 -1.2
68,028 + Psyr_0067 0.32 -0.1
68,028 + Psyr_0067 0.32 -0.3
68,028 + Psyr_0067 0.32 +1.2
68,028 + Psyr_0067 0.32 +1.3
68,029 - Psyr_0067 0.32 -2.6
68,029 - Psyr_0067 0.32 +0.1
68,029 - Psyr_0067 0.32 -1.7
68,066 + Psyr_0067 0.35 +1.1
68,066 + Psyr_0067 0.35 +0.1
68,067 - Psyr_0067 0.35 +0.3
68,072 + Psyr_0067 0.35 -0.0
68,072 + Psyr_0067 0.35 -1.1
68,072 + Psyr_0067 0.35 +1.0
68,073 - Psyr_0067 0.35 +0.4
68,073 - Psyr_0067 0.35 +0.2
68,115 + Psyr_0067 0.39 -0.0
68,115 + Psyr_0067 0.39 -0.3
68,116 - Psyr_0067 0.39 -0.1
68,116 - Psyr_0067 0.39 -0.0
68,311 + Psyr_0067 0.55 -0.5
68,312 - Psyr_0067 0.55 -0.7
68,339 + Psyr_0067 0.58 -1.1
68,339 + Psyr_0067 0.58 -0.3
68,340 - Psyr_0067 0.58 -0.1
68,453 + Psyr_0067 0.67 -0.2
68,453 + Psyr_0067 0.67 -0.2
68,453 + Psyr_0067 0.67 -0.3
68,453 + Psyr_0067 0.67 +0.5
68,454 - Psyr_0067 0.67 -1.0
68,454 - Psyr_0067 0.67 +0.3
68,454 - Psyr_0067 0.67 +1.2
68,454 - Psyr_0067 0.67 +0.4
68,456 + Psyr_0067 0.67 -0.9
68,456 + Psyr_0067 0.67 -0.7
68,456 + Psyr_0067 0.67 -0.2
68,456 + Psyr_0067 0.67 -0.1
68,456 + Psyr_0067 0.67 -0.8
68,457 - Psyr_0067 0.68 -1.3
68,457 - Psyr_0067 0.68 -0.8
68,457 - Psyr_0067 0.68 -0.7

Or see this region's nucleotide sequence