Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0012 and Psyr_0013 are separated by 20 nucleotides Psyr_0013 and Psyr_0014 are separated by 38 nucleotides
Psyr_0012: Psyr_0012 - glycyl-tRNA synthetase alpha chain, at 13,967 to 14,974
_0012
Psyr_0013: Psyr_0013 - DNA-3-methyladenine glycosylase I, at 14,995 to 15,546
_0013
Psyr_0014: Psyr_0014 - lipid A biosynthesis acyltransferase, at 15,585 to 16,472
_0014
Position (kb)
14
15
16 Strain fitness (log2 ratio)
-1
0
1
2
3 at 14.956 kb on - strand at 14.956 kb on - strand at 14.994 kb on + strand at 15.031 kb on + strand at 15.031 kb on + strand at 15.031 kb on + strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.040 kb on + strand at 15.040 kb on + strand at 15.040 kb on + strand at 15.040 kb on + strand at 15.041 kb on - strand at 15.041 kb on - strand at 15.170 kb on + strand, within Psyr_0013 at 15.171 kb on - strand, within Psyr_0013 at 15.171 kb on - strand, within Psyr_0013 at 15.171 kb on - strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.476 kb on - strand, within Psyr_0013 at 15.476 kb on - strand, within Psyr_0013 at 15.591 kb on - strand at 15.975 kb on + strand, within Psyr_0014 at 15.975 kb on + strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.335 kb on + strand, within Psyr_0014 at 16.335 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.389 kb on + strand at 16.389 kb on + strand at 16.413 kb on + strand at 16.413 kb on + strand at 16.413 kb on + strand at 16.413 kb on + strand at 16.414 kb on - strand at 16.414 kb on - strand at 16.467 kb on + strand at 16.467 kb on + strand at 16.470 kb on + strand at 16.471 kb on - strand at 16.471 kb on - strand at 16.471 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 14,956 - +0.8 14,956 - -1.6 14,994 + +0.9 15,031 + -1.0 15,031 + +1.2 15,031 + +1.1 15,032 - +1.6 15,032 - -0.7 15,032 - -0.7 15,032 - -0.1 15,032 - +2.0 15,032 - +0.0 15,032 - +3.0 15,032 - -1.1 15,032 - -1.0 15,040 + -0.2 15,040 + +0.2 15,040 + +1.3 15,040 + -0.8 15,041 - -0.4 15,041 - +0.3 15,170 + Psyr_0013 0.32 -0.4 15,171 - Psyr_0013 0.32 -1.5 15,171 - Psyr_0013 0.32 +0.6 15,171 - Psyr_0013 0.32 -0.3 15,475 + Psyr_0013 0.87 +1.2 15,475 + Psyr_0013 0.87 +0.4 15,475 + Psyr_0013 0.87 -0.4 15,475 + Psyr_0013 0.87 +1.4 15,475 + Psyr_0013 0.87 +1.0 15,475 + Psyr_0013 0.87 -0.1 15,475 + Psyr_0013 0.87 +0.9 15,476 - Psyr_0013 0.87 +1.1 15,476 - Psyr_0013 0.87 -0.1 15,591 - +0.4 15,975 + Psyr_0014 0.44 -0.0 15,975 + Psyr_0014 0.44 +2.0 16,302 - Psyr_0014 0.81 +2.0 16,302 - Psyr_0014 0.81 +0.4 16,302 - Psyr_0014 0.81 +2.0 16,335 + Psyr_0014 0.84 +2.0 16,335 - Psyr_0014 0.84 +2.0 16,336 - Psyr_0014 0.85 -0.3 16,336 - Psyr_0014 0.85 +2.0 16,336 - Psyr_0014 0.85 -0.0 16,336 - Psyr_0014 0.85 +1.0 16,336 - Psyr_0014 0.85 +1.0 16,336 - Psyr_0014 0.85 +0.4 16,389 + +2.0 16,389 + -0.3 16,413 + +0.4 16,413 + +2.0 16,413 + -0.6 16,413 + -0.0 16,414 - +2.0 16,414 - +2.0 16,467 + +0.4 16,467 + -0.0 16,470 + +2.1 16,471 - -0.7 16,471 - +0.0 16,471 - +1.1
Or see this region's nucleotide sequence