Experiment: H2 McCas with 1 hour oxygen exposure
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08730 and MMP_RS08735 are separated by 99 nucleotides MMP_RS08735 and MMP_RS08740 are separated by 187 nucleotides MMP_RS08740 and MMP_RS08745 are separated by 697 nucleotides
MMP_RS08730: MMP_RS08730 - F420-non-reducing hydrogenase subunit, at 1,635,430 to 1,636,296
_RS08730
MMP_RS08735: MMP_RS08735 - hydrogenase iron-sulfur subunit, at 1,636,396 to 1,636,800
_RS08735
MMP_RS08740: MMP_RS08740 - CoB--CoM heterodisulfide reductase iron-sulfur subunit A family protein, at 1,636,988 to 1,638,964
_RS08740
MMP_RS08745: MMP_RS08745 - CDP-2 3-bis-(O-geranylgeranyl)-sn-glycerol synthase, at 1,639,662 to 1,640,198
_RS08745
Position (kb)
1636
1637
1638
1639 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1635.999 kb on + strand at 1636.112 kb on + strand at 1636.783 kb on + strand at 1637.178 kb on - strand at 1637.198 kb on - strand at 1637.199 kb on + strand at 1637.199 kb on + strand at 1637.217 kb on + strand at 1637.819 kb on - strand at 1638.155 kb on + strand at 1638.162 kb on + strand at 1638.191 kb on - strand at 1638.247 kb on + strand at 1638.456 kb on - strand at 1638.509 kb on + strand at 1638.527 kb on + strand at 1639.009 kb on - strand at 1639.010 kb on + strand at 1639.011 kb on - strand at 1639.011 kb on - strand at 1639.039 kb on + strand at 1639.206 kb on + strand at 1639.217 kb on + strand at 1639.245 kb on + strand at 1639.275 kb on - strand at 1639.276 kb on + strand at 1639.277 kb on - strand at 1639.278 kb on + strand at 1639.278 kb on + strand at 1639.279 kb on - strand at 1639.280 kb on + strand at 1639.280 kb on + strand at 1639.298 kb on - strand at 1639.339 kb on - strand at 1639.373 kb on + strand at 1639.421 kb on + strand at 1639.439 kb on + strand at 1639.439 kb on + strand at 1639.451 kb on - strand at 1639.452 kb on + strand at 1639.516 kb on - strand at 1639.521 kb on - strand at 1639.522 kb on + strand at 1639.522 kb on + strand at 1639.525 kb on - strand at 1639.526 kb on + strand at 1639.593 kb on + strand at 1639.605 kb on - strand at 1639.605 kb on - strand at 1639.625 kb on - strand at 1639.625 kb on - strand at 1639.626 kb on + strand at 1639.626 kb on + strand at 1639.630 kb on - strand at 1639.631 kb on + strand at 1639.631 kb on + strand at 1639.681 kb on - strand at 1639.699 kb on + strand at 1639.713 kb on - strand at 1639.714 kb on + strand at 1639.717 kb on - strand, within MMP_RS08745 at 1639.736 kb on - strand, within MMP_RS08745 at 1639.810 kb on - strand, within MMP_RS08745 at 1639.874 kb on - strand, within MMP_RS08745 at 1639.880 kb on - strand, within MMP_RS08745 at 1639.881 kb on + strand, within MMP_RS08745 at 1639.882 kb on - strand, within MMP_RS08745 at 1639.883 kb on + strand, within MMP_RS08745 at 1639.883 kb on + strand, within MMP_RS08745 at 1639.883 kb on + strand, within MMP_RS08745 at 1639.885 kb on - strand, within MMP_RS08745 at 1639.886 kb on + strand, within MMP_RS08745 at 1639.886 kb on + strand, within MMP_RS08745 at 1639.887 kb on - strand, within MMP_RS08745 at 1639.889 kb on - strand, within MMP_RS08745 at 1639.889 kb on - strand, within MMP_RS08745 at 1639.931 kb on - strand, within MMP_RS08745
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 1 hour oxygen exposure remove 1,635,999 + -1.1 1,636,112 + -3.6 1,636,783 + -1.4 1,637,178 - -1.5 1,637,198 - -2.5 1,637,199 + -2.8 1,637,199 + -2.7 1,637,217 + -1.4 1,637,819 - -2.3 1,638,155 + -1.7 1,638,162 + -1.6 1,638,191 - -2.5 1,638,247 + -1.6 1,638,456 - -2.9 1,638,509 + -1.7 1,638,527 + -0.8 1,639,009 - -1.8 1,639,010 + -1.8 1,639,011 - -1.2 1,639,011 - -3.2 1,639,039 + +0.9 1,639,206 + +0.8 1,639,217 + +1.5 1,639,245 + +0.9 1,639,275 - -0.6 1,639,276 + -0.3 1,639,277 - -0.4 1,639,278 + -0.5 1,639,278 + -0.3 1,639,279 - -0.5 1,639,280 + -0.2 1,639,280 + -1.5 1,639,298 - -0.1 1,639,339 - -2.7 1,639,373 + -1.2 1,639,421 + +0.8 1,639,439 + +1.0 1,639,439 + +1.4 1,639,451 - +0.1 1,639,452 + +1.4 1,639,516 - +0.4 1,639,521 - +0.9 1,639,522 + +1.5 1,639,522 + +0.9 1,639,525 - +1.8 1,639,526 + +1.7 1,639,593 + +1.5 1,639,605 - -1.1 1,639,605 - -1.1 1,639,625 - -2.0 1,639,625 - -2.1 1,639,626 + -1.7 1,639,626 + -0.3 1,639,630 - -1.7 1,639,631 + -2.4 1,639,631 + -3.0 1,639,681 - -4.6 1,639,699 + -1.5 1,639,713 - -1.6 1,639,714 + -3.6 1,639,717 - MMP_RS08745 0.10 -2.8 1,639,736 - MMP_RS08745 0.14 -1.9 1,639,810 - MMP_RS08745 0.28 -0.8 1,639,874 - MMP_RS08745 0.39 -3.2 1,639,880 - MMP_RS08745 0.41 -1.2 1,639,881 + MMP_RS08745 0.41 -2.3 1,639,882 - MMP_RS08745 0.41 -1.9 1,639,883 + MMP_RS08745 0.41 -3.1 1,639,883 + MMP_RS08745 0.41 -1.5 1,639,883 + MMP_RS08745 0.41 -2.3 1,639,885 - MMP_RS08745 0.42 -1.2 1,639,886 + MMP_RS08745 0.42 -1.7 1,639,886 + MMP_RS08745 0.42 -2.0 1,639,887 - MMP_RS08745 0.42 -1.4 1,639,889 - MMP_RS08745 0.42 -0.1 1,639,889 - MMP_RS08745 0.42 -0.9 1,639,931 - MMP_RS08745 0.50 -1.3
Or see this region's nucleotide sequence