Experiment: H2 McCas with 1 hour oxygen exposure
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08040 and MMP_RS08045 are separated by 10 nucleotides MMP_RS08045 and MMP_RS08050 are separated by 21 nucleotides MMP_RS08050 and MMP_RS08055 are separated by 16 nucleotides MMP_RS08055 and MMP_RS08060 are separated by 4 nucleotides
MMP_RS08040: MMP_RS08040 - tetrahydromethanopterin S-methyltransferase subunit C, at 1,516,564 to 1,517,352
_RS08040
MMP_RS08045: MMP_RS08045 - tetrahydromethanopterin S-methyltransferase subunit B, at 1,517,363 to 1,517,689
_RS08045
MMP_RS08050: MMP_RS08050 - tetrahydromethanopterin S-methyltransferase subunit A, at 1,517,711 to 1,518,430
_RS08050
MMP_RS08055: MMP_RS08055 - tetrahydromethanopterin S-methyltransferase subunit A, at 1,518,447 to 1,519,208
_RS08055
MMP_RS08060: MMP_RS08060 - tetrahydromethanopterin S-methyltransferase subunit G, at 1,519,213 to 1,519,437
_RS08060
Position (kb)
1517
1518
1519 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1516.763 kb on - strand at 1516.794 kb on + strand at 1516.869 kb on - strand at 1516.972 kb on + strand at 1517.234 kb on - strand at 1517.235 kb on + strand at 1517.270 kb on + strand at 1517.320 kb on + strand at 1517.323 kb on - strand at 1517.324 kb on + strand at 1517.379 kb on - strand at 1517.379 kb on - strand at 1517.410 kb on + strand at 1517.644 kb on + strand at 1518.362 kb on - strand at 1518.391 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 1 hour oxygen exposure remove 1,516,763 - -1.8 1,516,794 + -0.8 1,516,869 - +0.1 1,516,972 + -1.5 1,517,234 - -1.0 1,517,235 + -1.9 1,517,270 + -2.5 1,517,320 + -0.9 1,517,323 - -3.0 1,517,324 + -0.0 1,517,379 - -0.9 1,517,379 - -1.1 1,517,410 + -1.9 1,517,644 + +0.1 1,518,362 - -2.8 1,518,391 + -0.8
Or see this region's nucleotide sequence