Experiment: H2 McCas with 1 hour oxygen exposure
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08015 and MMP_RS08020 are separated by 9 nucleotides MMP_RS08020 and MMP_RS08025 are separated by 47 nucleotides MMP_RS08025 and MMP_RS08030 are separated by 267 nucleotides
MMP_RS08015: MMP_RS08015 - methyl-coenzyme M reductase I operon protein C, at 1,511,528 to 1,512,124
_RS08015
MMP_RS08020: MMP_RS08020 - coenzyme-B sulfoethylthiotransferase subunit gamma, at 1,512,134 to 1,512,916
_RS08020
MMP_RS08025: MMP_RS08025 - coenzyme-B sulfoethylthiotransferase subunit alpha, at 1,512,964 to 1,514,625
_RS08025
MMP_RS08030: MMP_RS08030 - tetrahydromethanopterin S-methyltransferase subunit E, at 1,514,893 to 1,515,792
_RS08030
Position (kb)
1512
1513
1514
1515 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1512.118 kb on + strand at 1512.881 kb on - strand at 1513.887 kb on + strand at 1513.939 kb on - strand at 1514.018 kb on + strand at 1514.147 kb on - strand at 1514.211 kb on + strand at 1514.381 kb on - strand at 1514.624 kb on + strand at 1514.664 kb on - strand at 1514.666 kb on - strand at 1514.670 kb on - strand at 1514.703 kb on + strand at 1514.780 kb on - strand at 1515.049 kb on - strand at 1515.095 kb on + strand at 1515.234 kb on + strand at 1515.303 kb on + strand at 1515.316 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 1 hour oxygen exposure remove 1,512,118 + -3.0 1,512,881 - +0.1 1,513,887 + -2.0 1,513,939 - -3.2 1,514,018 + -1.8 1,514,147 - -2.7 1,514,211 + -2.5 1,514,381 - -2.0 1,514,624 + -1.0 1,514,664 - -1.2 1,514,666 - -0.5 1,514,670 - -1.0 1,514,703 + -0.3 1,514,780 - -2.5 1,515,049 - -2.8 1,515,095 + -1.6 1,515,234 + -1.0 1,515,303 + -0.9 1,515,316 + -1.6
Or see this region's nucleotide sequence