Experiment: H2 McCas with 1 hour oxygen exposure
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08000 and MMP_RS08005 are separated by 259 nucleotides MMP_RS08005 and MMP_RS08010 are separated by 11 nucleotides MMP_RS08010 and MMP_RS08015 are separated by 8 nucleotides
MMP_RS08000: MMP_RS08000 - methyl coenzyme M reductase-arginine methyltransferase Mmp10, at 1,508,127 to 1,509,437
_RS08000
MMP_RS08005: MMP_RS08005 - coenzyme-B sulfoethylthiotransferase subunit beta, at 1,509,697 to 1,511,028
_RS08005
MMP_RS08010: MMP_RS08010 - methyl-coenzyme M reductase operon protein D, at 1,511,040 to 1,511,519
_RS08010
MMP_RS08015: MMP_RS08015 - methyl-coenzyme M reductase I operon protein C, at 1,511,528 to 1,512,124
_RS08015
Position (kb)
1509
1510
1511
1512 Strain fitness (log2 ratio)
-2
-1
0
1 at 1508.702 kb on - strand, within MMP_RS08000 at 1508.720 kb on - strand, within MMP_RS08000 at 1508.790 kb on - strand, within MMP_RS08000 at 1508.791 kb on + strand, within MMP_RS08000 at 1508.974 kb on - strand, within MMP_RS08000 at 1509.014 kb on - strand, within MMP_RS08000 at 1509.014 kb on - strand, within MMP_RS08000 at 1509.015 kb on + strand, within MMP_RS08000 at 1509.016 kb on - strand, within MMP_RS08000 at 1509.021 kb on - strand, within MMP_RS08000 at 1509.021 kb on - strand, within MMP_RS08000 at 1509.237 kb on + strand, within MMP_RS08000 at 1509.438 kb on + strand at 1509.449 kb on + strand at 1509.452 kb on - strand at 1509.453 kb on + strand at 1509.467 kb on + strand at 1509.502 kb on - strand at 1509.533 kb on - strand at 1509.549 kb on + strand at 1509.604 kb on + strand at 1509.604 kb on + strand at 1509.642 kb on - strand at 1509.642 kb on - strand at 1509.643 kb on + strand at 1509.668 kb on - strand at 1509.670 kb on - strand at 1509.696 kb on - strand at 1509.862 kb on + strand at 1510.260 kb on + strand at 1511.447 kb on + strand at 1511.836 kb on - strand at 1511.853 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 1 hour oxygen exposure remove 1,508,702 - MMP_RS08000 0.44 +0.0 1,508,720 - MMP_RS08000 0.45 +0.7 1,508,790 - MMP_RS08000 0.51 -0.4 1,508,791 + MMP_RS08000 0.51 -0.1 1,508,974 - MMP_RS08000 0.65 +1.0 1,509,014 - MMP_RS08000 0.68 -0.4 1,509,014 - MMP_RS08000 0.68 -0.1 1,509,015 + MMP_RS08000 0.68 +0.0 1,509,016 - MMP_RS08000 0.68 +0.1 1,509,021 - MMP_RS08000 0.68 +0.7 1,509,021 - MMP_RS08000 0.68 -0.3 1,509,237 + MMP_RS08000 0.85 +0.5 1,509,438 + +0.2 1,509,449 + -1.6 1,509,452 - +1.2 1,509,453 + -0.6 1,509,467 + +1.0 1,509,502 - +0.8 1,509,533 - -1.0 1,509,549 + +0.2 1,509,604 + +0.4 1,509,604 + -0.1 1,509,642 - -1.7 1,509,642 - -1.6 1,509,643 + -1.0 1,509,668 - -2.2 1,509,670 - -1.2 1,509,696 - -1.3 1,509,862 + -1.4 1,510,260 + -0.6 1,511,447 + -0.3 1,511,836 - -1.0 1,511,853 - -1.9
Or see this region's nucleotide sequence