Experiment: H2 McCas with 1 hour oxygen exposure
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06395 and MMP_RS06400 are separated by 203 nucleotides MMP_RS06400 and MMP_RS06405 are separated by 23 nucleotides MMP_RS06405 and MMP_RS06410 are separated by 22 nucleotides MMP_RS06410 and MMP_RS06415 are separated by 41 nucleotides MMP_RS06415 and MMP_RS06420 are separated by 22 nucleotides
MMP_RS06395: MMP_RS06395 - ThiF family adenylyltransferase, at 1,228,155 to 1,228,769
_RS06395
MMP_RS06400: MMP_RS06400 - 4Fe-4S binding protein, at 1,228,973 to 1,229,419
_RS06400
MMP_RS06405: MMP_RS06405 - 4Fe-4S binding protein, at 1,229,443 to 1,230,501
_RS06405
MMP_RS06410: MMP_RS06410 - 4Fe-4S binding protein, at 1,230,524 to 1,230,766
_RS06410
MMP_RS06415: MMP_RS06415 - protein fwdD, at 1,230,808 to 1,231,194
_RS06415
MMP_RS06420: MMP_RS06420 - formylmethanofuran dehydrogenase subunit A, at 1,231,217 to 1,232,920
_RS06420
Position (kb)
1229
1230
1231 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1228.509 kb on + strand, within MMP_RS06395 at 1228.568 kb on + strand, within MMP_RS06395 at 1228.603 kb on + strand, within MMP_RS06395 at 1228.798 kb on + strand at 1228.837 kb on + strand at 1228.928 kb on - strand at 1228.929 kb on + strand at 1228.929 kb on + strand at 1228.929 kb on + strand at 1228.929 kb on + strand at 1228.951 kb on + strand at 1228.985 kb on + strand at 1228.985 kb on + strand at 1228.985 kb on + strand at 1228.986 kb on - strand at 1228.986 kb on - strand at 1228.987 kb on + strand at 1228.987 kb on + strand at 1228.990 kb on + strand at 1229.234 kb on - strand, within MMP_RS06400 at 1229.242 kb on + strand, within MMP_RS06400 at 1229.251 kb on - strand, within MMP_RS06400 at 1229.439 kb on - strand at 1229.440 kb on + strand at 1230.027 kb on + strand at 1230.083 kb on + strand at 1230.357 kb on + strand at 1230.445 kb on + strand at 1230.540 kb on - strand at 1230.541 kb on + strand at 1230.612 kb on - strand at 1230.613 kb on + strand at 1230.685 kb on + strand at 1230.959 kb on - strand at 1231.035 kb on + strand at 1231.170 kb on + strand at 1231.184 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 1 hour oxygen exposure remove 1,228,509 + MMP_RS06395 0.58 -0.3 1,228,568 + MMP_RS06395 0.67 -1.5 1,228,603 + MMP_RS06395 0.73 -3.4 1,228,798 + +1.2 1,228,837 + +0.1 1,228,928 - -2.7 1,228,929 + -3.7 1,228,929 + -3.5 1,228,929 + -2.5 1,228,929 + -2.0 1,228,951 + -1.9 1,228,985 + -2.5 1,228,985 + -3.5 1,228,985 + -4.6 1,228,986 - -1.7 1,228,986 - -2.8 1,228,987 + -2.7 1,228,987 + -1.6 1,228,990 + -1.0 1,229,234 - MMP_RS06400 0.58 -1.7 1,229,242 + MMP_RS06400 0.60 -2.8 1,229,251 - MMP_RS06400 0.62 -2.8 1,229,439 - -1.7 1,229,440 + -1.7 1,230,027 + -1.9 1,230,083 + -2.5 1,230,357 + -1.4 1,230,445 + -2.7 1,230,540 - -1.2 1,230,541 + -2.2 1,230,612 - -1.1 1,230,613 + -3.7 1,230,685 + -2.9 1,230,959 - -0.5 1,231,035 + -2.3 1,231,170 + -1.6 1,231,184 + -3.8
Or see this region's nucleotide sequence