Experiment: H2 McCas with 1 hour oxygen exposure
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06125 and MMP_RS06130 are separated by 68 nucleotides MMP_RS06130 and MMP_RS06135 are separated by 190 nucleotides MMP_RS06135 and MMP_RS06140 are separated by 119 nucleotides
MMP_RS06125: MMP_RS06125 - ribose-5-phosphate isomerase RpiA, at 1,171,728 to 1,172,447
_RS06125
MMP_RS06130: MMP_RS06130 - peptidylprolyl isomerase, at 1,172,516 to 1,172,968
_RS06130
MMP_RS06135: MMP_RS06135 - methenyltetrahydromethanopterin cyclohydrolase, at 1,173,159 to 1,174,130
_RS06135
MMP_RS06140: MMP_RS06140 - radical SAM protein, at 1,174,250 to 1,175,365
_RS06140
Position (kb)
1173
1174
1175 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1172.164 kb on + strand at 1172.220 kb on + strand at 1172.249 kb on - strand at 1172.249 kb on - strand at 1172.250 kb on + strand at 1172.264 kb on - strand at 1172.265 kb on + strand at 1172.436 kb on + strand at 1172.495 kb on + strand at 1172.643 kb on + strand, within MMP_RS06130 at 1172.756 kb on - strand, within MMP_RS06130 at 1172.844 kb on - strand, within MMP_RS06130 at 1172.844 kb on - strand, within MMP_RS06130 at 1172.845 kb on + strand, within MMP_RS06130 at 1172.882 kb on - strand, within MMP_RS06130 at 1172.951 kb on + strand at 1172.982 kb on - strand at 1173.017 kb on - strand at 1173.017 kb on - strand at 1173.017 kb on - strand at 1173.021 kb on + strand at 1173.023 kb on + strand at 1173.023 kb on + strand at 1173.023 kb on + strand at 1173.023 kb on + strand at 1173.023 kb on + strand at 1173.023 kb on + strand at 1173.023 kb on + strand at 1173.023 kb on + strand at 1173.024 kb on - strand at 1173.024 kb on - strand at 1173.025 kb on + strand at 1173.026 kb on - strand at 1173.027 kb on + strand at 1173.049 kb on - strand at 1173.050 kb on + strand at 1173.054 kb on - strand at 1173.137 kb on + strand at 1173.165 kb on + strand at 1173.262 kb on - strand at 1173.397 kb on - strand at 1173.920 kb on - strand at 1173.967 kb on - strand at 1174.096 kb on - strand at 1174.128 kb on - strand at 1174.139 kb on + strand at 1174.145 kb on - strand at 1174.149 kb on - strand at 1174.152 kb on + strand at 1174.156 kb on + strand at 1174.157 kb on - strand at 1174.157 kb on - strand at 1174.178 kb on - strand at 1174.178 kb on - strand at 1174.327 kb on + strand at 1174.329 kb on + strand at 1174.358 kb on - strand at 1174.526 kb on - strand, within MMP_RS06140 at 1174.794 kb on - strand, within MMP_RS06140 at 1174.906 kb on - strand, within MMP_RS06140 at 1174.907 kb on + strand, within MMP_RS06140 at 1174.949 kb on + strand, within MMP_RS06140 at 1175.091 kb on - strand, within MMP_RS06140
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 1 hour oxygen exposure remove 1,172,164 + -2.8 1,172,220 + -1.7 1,172,249 - -0.7 1,172,249 - -1.7 1,172,250 + -3.3 1,172,264 - -1.1 1,172,265 + -1.8 1,172,436 + -3.5 1,172,495 + -1.0 1,172,643 + MMP_RS06130 0.28 -1.8 1,172,756 - MMP_RS06130 0.53 -0.3 1,172,844 - MMP_RS06130 0.72 -1.0 1,172,844 - MMP_RS06130 0.72 -1.1 1,172,845 + MMP_RS06130 0.73 -1.4 1,172,882 - MMP_RS06130 0.81 -0.3 1,172,951 + -3.3 1,172,982 - -0.0 1,173,017 - -0.6 1,173,017 - -0.2 1,173,017 - -0.3 1,173,021 + -1.3 1,173,023 + -1.3 1,173,023 + -1.2 1,173,023 + -1.0 1,173,023 + -1.1 1,173,023 + -0.5 1,173,023 + -0.5 1,173,023 + -0.7 1,173,023 + -0.7 1,173,024 - +0.6 1,173,024 - -0.6 1,173,025 + +0.8 1,173,026 - -0.2 1,173,027 + +0.9 1,173,049 - +0.5 1,173,050 + +0.1 1,173,054 - -0.1 1,173,137 + -2.8 1,173,165 + -1.6 1,173,262 - -1.6 1,173,397 - -1.6 1,173,920 - -2.3 1,173,967 - -0.2 1,174,096 - -3.9 1,174,128 - -0.0 1,174,139 + +0.2 1,174,145 - +0.1 1,174,149 - -0.0 1,174,152 + +0.2 1,174,156 + +1.3 1,174,157 - +0.4 1,174,157 - +0.4 1,174,178 - -1.1 1,174,178 - -0.8 1,174,327 + +0.7 1,174,329 + -0.8 1,174,358 - -0.5 1,174,526 - MMP_RS06140 0.25 -0.8 1,174,794 - MMP_RS06140 0.49 +0.3 1,174,906 - MMP_RS06140 0.59 +0.9 1,174,907 + MMP_RS06140 0.59 -2.5 1,174,949 + MMP_RS06140 0.63 +0.8 1,175,091 - MMP_RS06140 0.75 +0.2
Or see this region's nucleotide sequence