Experiment: LB in EcoFAB_3.5mL with sand
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26845 and BWI76_RS26850 overlap by 4 nucleotides BWI76_RS26850 and BWI76_RS26855 are separated by 20 nucleotides BWI76_RS26855 and BWI76_RS26860 overlap by 10 nucleotides
BWI76_RS26845: BWI76_RS26845 - cellulose synthase regulator BcsB, at 5,566,917 to 5,569,319
_RS26845
BWI76_RS26850: BWI76_RS26850 - cellulose synthase catalytic subunit (UDP-forming), at 5,569,316 to 5,571,427
_RS26850
BWI76_RS26855: BWI76_RS26855 - cellulose synthase operon protein YhjQ, at 5,571,448 to 5,572,251
_RS26855
BWI76_RS26860: BWI76_RS26860 - hypothetical protein, at 5,572,242 to 5,572,799
_RS26860
Position (kb)
5569
5570
5571
5572 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5568.721 kb on + strand, within BWI76_RS26845 at 5568.731 kb on + strand, within BWI76_RS26845 at 5568.965 kb on + strand, within BWI76_RS26845 at 5569.334 kb on - strand at 5569.336 kb on + strand at 5569.392 kb on + strand at 5569.392 kb on + strand at 5569.392 kb on + strand at 5569.400 kb on - strand at 5569.400 kb on - strand at 5569.472 kb on - strand at 5569.495 kb on - strand at 5569.587 kb on + strand, within BWI76_RS26850 at 5569.618 kb on + strand, within BWI76_RS26850 at 5569.626 kb on - strand, within BWI76_RS26850 at 5569.969 kb on + strand, within BWI76_RS26850 at 5570.048 kb on - strand, within BWI76_RS26850 at 5570.276 kb on + strand, within BWI76_RS26850 at 5570.300 kb on - strand, within BWI76_RS26850 at 5570.308 kb on + strand, within BWI76_RS26850 at 5570.573 kb on + strand, within BWI76_RS26850 at 5570.624 kb on - strand, within BWI76_RS26850 at 5570.761 kb on + strand, within BWI76_RS26850 at 5570.798 kb on + strand, within BWI76_RS26850 at 5570.838 kb on - strand, within BWI76_RS26850 at 5570.881 kb on - strand, within BWI76_RS26850 at 5570.884 kb on + strand, within BWI76_RS26850 at 5571.097 kb on + strand, within BWI76_RS26850 at 5571.114 kb on + strand, within BWI76_RS26850 at 5571.127 kb on + strand, within BWI76_RS26850 at 5571.145 kb on - strand, within BWI76_RS26850 at 5571.155 kb on + strand, within BWI76_RS26850 at 5571.175 kb on - strand, within BWI76_RS26850 at 5571.304 kb on + strand at 5571.348 kb on - strand at 5571.371 kb on - strand at 5571.453 kb on + strand at 5571.453 kb on + strand at 5571.453 kb on + strand at 5571.461 kb on - strand at 5571.461 kb on - strand at 5571.461 kb on - strand at 5571.472 kb on + strand at 5571.480 kb on - strand at 5571.480 kb on - strand at 5571.480 kb on - strand at 5571.481 kb on - strand at 5571.583 kb on + strand, within BWI76_RS26855 at 5571.601 kb on - strand, within BWI76_RS26855 at 5571.625 kb on + strand, within BWI76_RS26855 at 5571.703 kb on + strand, within BWI76_RS26855 at 5571.732 kb on + strand, within BWI76_RS26855 at 5571.744 kb on + strand, within BWI76_RS26855 at 5571.868 kb on - strand, within BWI76_RS26855 at 5571.952 kb on + strand, within BWI76_RS26855 at 5572.052 kb on - strand, within BWI76_RS26855 at 5572.179 kb on - strand at 5572.194 kb on + strand at 5572.204 kb on + strand at 5572.240 kb on + strand at 5572.308 kb on - strand, within BWI76_RS26860 at 5572.337 kb on - strand, within BWI76_RS26860 at 5572.349 kb on - strand, within BWI76_RS26860 at 5572.398 kb on - strand, within BWI76_RS26860
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL with sand remove 5,568,721 + BWI76_RS26845 0.75 -1.6 5,568,731 + BWI76_RS26845 0.75 -0.4 5,568,965 + BWI76_RS26845 0.85 -0.8 5,569,334 - +0.1 5,569,336 + +0.8 5,569,392 + +2.2 5,569,392 + -0.7 5,569,392 + +0.8 5,569,400 - -1.1 5,569,400 - -0.5 5,569,472 - -0.9 5,569,495 - +0.6 5,569,587 + BWI76_RS26850 0.13 -0.5 5,569,618 + BWI76_RS26850 0.14 -2.0 5,569,626 - BWI76_RS26850 0.15 -1.0 5,569,969 + BWI76_RS26850 0.31 -0.4 5,570,048 - BWI76_RS26850 0.35 -1.1 5,570,276 + BWI76_RS26850 0.45 -1.5 5,570,300 - BWI76_RS26850 0.47 -0.1 5,570,308 + BWI76_RS26850 0.47 -1.5 5,570,573 + BWI76_RS26850 0.60 +1.3 5,570,624 - BWI76_RS26850 0.62 -0.6 5,570,761 + BWI76_RS26850 0.68 -1.9 5,570,798 + BWI76_RS26850 0.70 -0.8 5,570,838 - BWI76_RS26850 0.72 +0.0 5,570,881 - BWI76_RS26850 0.74 -0.7 5,570,884 + BWI76_RS26850 0.74 -1.7 5,571,097 + BWI76_RS26850 0.84 -1.7 5,571,114 + BWI76_RS26850 0.85 -1.4 5,571,127 + BWI76_RS26850 0.86 -0.7 5,571,145 - BWI76_RS26850 0.87 -1.7 5,571,155 + BWI76_RS26850 0.87 +0.6 5,571,175 - BWI76_RS26850 0.88 -0.7 5,571,304 + -0.5 5,571,348 - -1.5 5,571,371 - -0.5 5,571,453 + -0.7 5,571,453 + -0.8 5,571,453 + -0.1 5,571,461 - -0.8 5,571,461 - +0.2 5,571,461 - -0.4 5,571,472 + -0.5 5,571,480 - +0.6 5,571,480 - -0.8 5,571,480 - +2.6 5,571,481 - +1.0 5,571,583 + BWI76_RS26855 0.17 -1.8 5,571,601 - BWI76_RS26855 0.19 -2.1 5,571,625 + BWI76_RS26855 0.22 -1.9 5,571,703 + BWI76_RS26855 0.32 -1.3 5,571,732 + BWI76_RS26855 0.35 -1.0 5,571,744 + BWI76_RS26855 0.37 -1.4 5,571,868 - BWI76_RS26855 0.52 -0.2 5,571,952 + BWI76_RS26855 0.63 -0.4 5,572,052 - BWI76_RS26855 0.75 +0.1 5,572,179 - -0.6 5,572,194 + -0.3 5,572,204 + -0.4 5,572,240 + -0.3 5,572,308 - BWI76_RS26860 0.12 -1.1 5,572,337 - BWI76_RS26860 0.17 -0.2 5,572,349 - BWI76_RS26860 0.19 -0.2 5,572,398 - BWI76_RS26860 0.28 -0.5
Or see this region's nucleotide sequence