Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26850

Experiment: LB in EcoFAB_3.5mL with sand

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26845 and BWI76_RS26850 overlap by 4 nucleotidesBWI76_RS26850 and BWI76_RS26855 are separated by 20 nucleotidesBWI76_RS26855 and BWI76_RS26860 overlap by 10 nucleotides BWI76_RS26845: BWI76_RS26845 - cellulose synthase regulator BcsB, at 5,566,917 to 5,569,319 _RS26845 BWI76_RS26850: BWI76_RS26850 - cellulose synthase catalytic subunit (UDP-forming), at 5,569,316 to 5,571,427 _RS26850 BWI76_RS26855: BWI76_RS26855 - cellulose synthase operon protein YhjQ, at 5,571,448 to 5,572,251 _RS26855 BWI76_RS26860: BWI76_RS26860 - hypothetical protein, at 5,572,242 to 5,572,799 _RS26860 Position (kb) 5569 5570 5571 5572Strain fitness (log2 ratio) -2 -1 0 1 2at 5568.721 kb on + strand, within BWI76_RS26845at 5568.731 kb on + strand, within BWI76_RS26845at 5568.965 kb on + strand, within BWI76_RS26845at 5569.334 kb on - strandat 5569.336 kb on + strandat 5569.392 kb on + strandat 5569.392 kb on + strandat 5569.392 kb on + strandat 5569.400 kb on - strandat 5569.400 kb on - strandat 5569.472 kb on - strandat 5569.495 kb on - strandat 5569.587 kb on + strand, within BWI76_RS26850at 5569.618 kb on + strand, within BWI76_RS26850at 5569.626 kb on - strand, within BWI76_RS26850at 5569.969 kb on + strand, within BWI76_RS26850at 5570.048 kb on - strand, within BWI76_RS26850at 5570.276 kb on + strand, within BWI76_RS26850at 5570.300 kb on - strand, within BWI76_RS26850at 5570.308 kb on + strand, within BWI76_RS26850at 5570.573 kb on + strand, within BWI76_RS26850at 5570.624 kb on - strand, within BWI76_RS26850at 5570.761 kb on + strand, within BWI76_RS26850at 5570.798 kb on + strand, within BWI76_RS26850at 5570.838 kb on - strand, within BWI76_RS26850at 5570.881 kb on - strand, within BWI76_RS26850at 5570.884 kb on + strand, within BWI76_RS26850at 5571.097 kb on + strand, within BWI76_RS26850at 5571.114 kb on + strand, within BWI76_RS26850at 5571.127 kb on + strand, within BWI76_RS26850at 5571.145 kb on - strand, within BWI76_RS26850at 5571.155 kb on + strand, within BWI76_RS26850at 5571.175 kb on - strand, within BWI76_RS26850at 5571.304 kb on + strandat 5571.348 kb on - strandat 5571.371 kb on - strandat 5571.453 kb on + strandat 5571.453 kb on + strandat 5571.453 kb on + strandat 5571.461 kb on - strandat 5571.461 kb on - strandat 5571.461 kb on - strandat 5571.472 kb on + strandat 5571.480 kb on - strandat 5571.480 kb on - strandat 5571.480 kb on - strandat 5571.481 kb on - strandat 5571.583 kb on + strand, within BWI76_RS26855at 5571.601 kb on - strand, within BWI76_RS26855at 5571.625 kb on + strand, within BWI76_RS26855at 5571.703 kb on + strand, within BWI76_RS26855at 5571.732 kb on + strand, within BWI76_RS26855at 5571.744 kb on + strand, within BWI76_RS26855at 5571.868 kb on - strand, within BWI76_RS26855at 5571.952 kb on + strand, within BWI76_RS26855at 5572.052 kb on - strand, within BWI76_RS26855at 5572.179 kb on - strandat 5572.194 kb on + strandat 5572.204 kb on + strandat 5572.240 kb on + strandat 5572.308 kb on - strand, within BWI76_RS26860at 5572.337 kb on - strand, within BWI76_RS26860at 5572.349 kb on - strand, within BWI76_RS26860at 5572.398 kb on - strand, within BWI76_RS26860

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Per-strain Table

Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL with sand
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5,568,721 + BWI76_RS26845 0.75 -1.6
5,568,731 + BWI76_RS26845 0.75 -0.4
5,568,965 + BWI76_RS26845 0.85 -0.8
5,569,334 - +0.1
5,569,336 + +0.8
5,569,392 + +2.2
5,569,392 + -0.7
5,569,392 + +0.8
5,569,400 - -1.1
5,569,400 - -0.5
5,569,472 - -0.9
5,569,495 - +0.6
5,569,587 + BWI76_RS26850 0.13 -0.5
5,569,618 + BWI76_RS26850 0.14 -2.0
5,569,626 - BWI76_RS26850 0.15 -1.0
5,569,969 + BWI76_RS26850 0.31 -0.4
5,570,048 - BWI76_RS26850 0.35 -1.1
5,570,276 + BWI76_RS26850 0.45 -1.5
5,570,300 - BWI76_RS26850 0.47 -0.1
5,570,308 + BWI76_RS26850 0.47 -1.5
5,570,573 + BWI76_RS26850 0.60 +1.3
5,570,624 - BWI76_RS26850 0.62 -0.6
5,570,761 + BWI76_RS26850 0.68 -1.9
5,570,798 + BWI76_RS26850 0.70 -0.8
5,570,838 - BWI76_RS26850 0.72 +0.0
5,570,881 - BWI76_RS26850 0.74 -0.7
5,570,884 + BWI76_RS26850 0.74 -1.7
5,571,097 + BWI76_RS26850 0.84 -1.7
5,571,114 + BWI76_RS26850 0.85 -1.4
5,571,127 + BWI76_RS26850 0.86 -0.7
5,571,145 - BWI76_RS26850 0.87 -1.7
5,571,155 + BWI76_RS26850 0.87 +0.6
5,571,175 - BWI76_RS26850 0.88 -0.7
5,571,304 + -0.5
5,571,348 - -1.5
5,571,371 - -0.5
5,571,453 + -0.7
5,571,453 + -0.8
5,571,453 + -0.1
5,571,461 - -0.8
5,571,461 - +0.2
5,571,461 - -0.4
5,571,472 + -0.5
5,571,480 - +0.6
5,571,480 - -0.8
5,571,480 - +2.6
5,571,481 - +1.0
5,571,583 + BWI76_RS26855 0.17 -1.8
5,571,601 - BWI76_RS26855 0.19 -2.1
5,571,625 + BWI76_RS26855 0.22 -1.9
5,571,703 + BWI76_RS26855 0.32 -1.3
5,571,732 + BWI76_RS26855 0.35 -1.0
5,571,744 + BWI76_RS26855 0.37 -1.4
5,571,868 - BWI76_RS26855 0.52 -0.2
5,571,952 + BWI76_RS26855 0.63 -0.4
5,572,052 - BWI76_RS26855 0.75 +0.1
5,572,179 - -0.6
5,572,194 + -0.3
5,572,204 + -0.4
5,572,240 + -0.3
5,572,308 - BWI76_RS26860 0.12 -1.1
5,572,337 - BWI76_RS26860 0.17 -0.2
5,572,349 - BWI76_RS26860 0.19 -0.2
5,572,398 - BWI76_RS26860 0.28 -0.5

Or see this region's nucleotide sequence