Experiment: LB in EcoFAB_3.5mL with sand
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26840 and BWI76_RS26845 overlap by 4 nucleotides BWI76_RS26845 and BWI76_RS26850 overlap by 4 nucleotides
BWI76_RS26840: BWI76_RS26840 - cellulose synthase, at 5,562,868 to 5,566,920
_RS26840
BWI76_RS26845: BWI76_RS26845 - cellulose synthase regulator BcsB, at 5,566,917 to 5,569,319
_RS26845
BWI76_RS26850: BWI76_RS26850 - cellulose synthase catalytic subunit (UDP-forming), at 5,569,316 to 5,571,427
_RS26850
Position (kb)
5566
5567
5568
5569
5570 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5565.968 kb on + strand, within BWI76_RS26840 at 5565.973 kb on + strand, within BWI76_RS26840 at 5565.981 kb on - strand, within BWI76_RS26840 at 5566.046 kb on - strand, within BWI76_RS26840 at 5566.080 kb on + strand, within BWI76_RS26840 at 5566.085 kb on + strand, within BWI76_RS26840 at 5566.085 kb on + strand, within BWI76_RS26840 at 5566.160 kb on - strand, within BWI76_RS26840 at 5566.198 kb on - strand, within BWI76_RS26840 at 5566.208 kb on + strand, within BWI76_RS26840 at 5566.321 kb on + strand, within BWI76_RS26840 at 5566.321 kb on + strand, within BWI76_RS26840 at 5566.429 kb on + strand, within BWI76_RS26840 at 5566.503 kb on + strand, within BWI76_RS26840 at 5566.542 kb on - strand at 5566.542 kb on - strand at 5566.705 kb on + strand at 5566.849 kb on - strand at 5566.867 kb on - strand at 5566.936 kb on - strand at 5567.124 kb on - strand at 5567.328 kb on + strand, within BWI76_RS26845 at 5567.518 kb on - strand, within BWI76_RS26845 at 5567.791 kb on - strand, within BWI76_RS26845 at 5567.810 kb on - strand, within BWI76_RS26845 at 5567.816 kb on + strand, within BWI76_RS26845 at 5567.911 kb on + strand, within BWI76_RS26845 at 5567.914 kb on - strand, within BWI76_RS26845 at 5567.965 kb on + strand, within BWI76_RS26845 at 5568.002 kb on + strand, within BWI76_RS26845 at 5568.239 kb on + strand, within BWI76_RS26845 at 5568.247 kb on - strand, within BWI76_RS26845 at 5568.281 kb on + strand, within BWI76_RS26845 at 5568.721 kb on + strand, within BWI76_RS26845 at 5568.731 kb on + strand, within BWI76_RS26845 at 5568.965 kb on + strand, within BWI76_RS26845 at 5569.334 kb on - strand at 5569.336 kb on + strand at 5569.392 kb on + strand at 5569.392 kb on + strand at 5569.392 kb on + strand at 5569.400 kb on - strand at 5569.400 kb on - strand at 5569.472 kb on - strand at 5569.495 kb on - strand at 5569.587 kb on + strand, within BWI76_RS26850 at 5569.618 kb on + strand, within BWI76_RS26850 at 5569.626 kb on - strand, within BWI76_RS26850 at 5569.969 kb on + strand, within BWI76_RS26850 at 5570.048 kb on - strand, within BWI76_RS26850 at 5570.276 kb on + strand, within BWI76_RS26850 at 5570.300 kb on - strand, within BWI76_RS26850 at 5570.308 kb on + strand, within BWI76_RS26850
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL with sand remove 5,565,968 + BWI76_RS26840 0.76 +1.3 5,565,973 + BWI76_RS26840 0.77 -0.4 5,565,981 - BWI76_RS26840 0.77 +1.9 5,566,046 - BWI76_RS26840 0.78 -0.5 5,566,080 + BWI76_RS26840 0.79 +0.2 5,566,085 + BWI76_RS26840 0.79 -0.4 5,566,085 + BWI76_RS26840 0.79 -1.0 5,566,160 - BWI76_RS26840 0.81 -2.1 5,566,198 - BWI76_RS26840 0.82 -0.4 5,566,208 + BWI76_RS26840 0.82 -0.5 5,566,321 + BWI76_RS26840 0.85 -0.2 5,566,321 + BWI76_RS26840 0.85 -0.5 5,566,429 + BWI76_RS26840 0.88 -0.1 5,566,503 + BWI76_RS26840 0.90 -1.1 5,566,542 - -0.7 5,566,542 - +0.0 5,566,705 + -0.6 5,566,849 - -0.4 5,566,867 - -1.1 5,566,936 - -0.3 5,567,124 - -0.9 5,567,328 + BWI76_RS26845 0.17 -1.1 5,567,518 - BWI76_RS26845 0.25 -0.5 5,567,791 - BWI76_RS26845 0.36 -0.7 5,567,810 - BWI76_RS26845 0.37 -2.4 5,567,816 + BWI76_RS26845 0.37 -1.0 5,567,911 + BWI76_RS26845 0.41 -1.0 5,567,914 - BWI76_RS26845 0.41 -0.7 5,567,965 + BWI76_RS26845 0.44 -0.8 5,568,002 + BWI76_RS26845 0.45 -0.1 5,568,239 + BWI76_RS26845 0.55 -0.7 5,568,247 - BWI76_RS26845 0.55 -0.8 5,568,281 + BWI76_RS26845 0.57 -0.3 5,568,721 + BWI76_RS26845 0.75 -1.6 5,568,731 + BWI76_RS26845 0.75 -0.4 5,568,965 + BWI76_RS26845 0.85 -0.8 5,569,334 - +0.1 5,569,336 + +0.8 5,569,392 + +2.2 5,569,392 + -0.7 5,569,392 + +0.8 5,569,400 - -1.1 5,569,400 - -0.5 5,569,472 - -0.9 5,569,495 - +0.6 5,569,587 + BWI76_RS26850 0.13 -0.5 5,569,618 + BWI76_RS26850 0.14 -2.0 5,569,626 - BWI76_RS26850 0.15 -1.0 5,569,969 + BWI76_RS26850 0.31 -0.4 5,570,048 - BWI76_RS26850 0.35 -1.1 5,570,276 + BWI76_RS26850 0.45 -1.5 5,570,300 - BWI76_RS26850 0.47 -0.1 5,570,308 + BWI76_RS26850 0.47 -1.5
Or see this region's nucleotide sequence