Experiment: LB in EcoFAB_3.5mL with sand
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17150 and BWI76_RS17155 overlap by 39 nucleotides BWI76_RS17155 and BWI76_RS17160 are separated by 42 nucleotides BWI76_RS17160 and BWI76_RS17165 are separated by 14 nucleotides BWI76_RS17165 and BWI76_RS17170 are separated by 270 nucleotides
BWI76_RS17150: BWI76_RS17150 - threonine--tRNA ligase, at 3,556,079 to 3,558,007
_RS17150
BWI76_RS17155: BWI76_RS17155 - hypothetical protein, at 3,557,969 to 3,558,181
_RS17155
BWI76_RS17160: BWI76_RS17160 - hypothetical protein, at 3,558,224 to 3,558,442
_RS17160
BWI76_RS17165: BWI76_RS17165 - hypothetical protein, at 3,558,457 to 3,558,690
_RS17165
BWI76_RS17170: BWI76_RS17170 - membrane protein, at 3,558,961 to 3,559,719
_RS17170
Position (kb)
3557
3558
3559 Strain fitness (log2 ratio)
-2
-1
0
1 at 3558.090 kb on - strand, within BWI76_RS17155 at 3558.122 kb on - strand, within BWI76_RS17155 at 3558.183 kb on - strand at 3558.188 kb on + strand at 3558.188 kb on + strand at 3558.188 kb on + strand at 3558.188 kb on + strand at 3558.188 kb on + strand at 3558.196 kb on - strand at 3558.196 kb on - strand at 3558.227 kb on - strand at 3558.308 kb on - strand, within BWI76_RS17160 at 3558.310 kb on + strand, within BWI76_RS17160 at 3558.333 kb on + strand, within BWI76_RS17160 at 3558.395 kb on + strand, within BWI76_RS17160 at 3558.395 kb on + strand, within BWI76_RS17160 at 3558.395 kb on + strand, within BWI76_RS17160 at 3558.395 kb on + strand, within BWI76_RS17160 at 3558.398 kb on - strand, within BWI76_RS17160 at 3558.402 kb on - strand, within BWI76_RS17160 at 3558.403 kb on - strand, within BWI76_RS17160 at 3558.403 kb on - strand, within BWI76_RS17160 at 3558.403 kb on - strand, within BWI76_RS17160 at 3558.403 kb on - strand, within BWI76_RS17160 at 3558.405 kb on + strand, within BWI76_RS17160 at 3558.405 kb on + strand, within BWI76_RS17160 at 3558.413 kb on - strand, within BWI76_RS17160 at 3558.443 kb on - strand at 3558.454 kb on - strand at 3558.455 kb on + strand at 3558.463 kb on + strand at 3558.472 kb on + strand at 3558.480 kb on + strand at 3558.502 kb on + strand, within BWI76_RS17165 at 3558.503 kb on + strand, within BWI76_RS17165 at 3558.511 kb on - strand, within BWI76_RS17165 at 3558.521 kb on - strand, within BWI76_RS17165 at 3558.534 kb on + strand, within BWI76_RS17165 at 3558.541 kb on + strand at 3558.547 kb on - strand, within BWI76_RS17165 at 3558.549 kb on - strand, within BWI76_RS17165 at 3558.577 kb on + strand, within BWI76_RS17165 at 3558.577 kb on + strand, within BWI76_RS17165 at 3558.592 kb on + strand, within BWI76_RS17165 at 3558.643 kb on - strand, within BWI76_RS17165 at 3558.643 kb on - strand, within BWI76_RS17165 at 3558.647 kb on + strand, within BWI76_RS17165 at 3558.652 kb on - strand, within BWI76_RS17165 at 3558.655 kb on - strand, within BWI76_RS17165 at 3558.736 kb on + strand at 3558.736 kb on + strand at 3558.744 kb on - strand at 3558.744 kb on - strand at 3558.744 kb on - strand at 3558.746 kb on + strand at 3558.746 kb on + strand at 3558.746 kb on + strand at 3558.746 kb on + strand at 3558.746 kb on + strand at 3558.746 kb on + strand at 3558.746 kb on + strand at 3558.746 kb on + strand at 3558.748 kb on + strand at 3558.754 kb on + strand at 3558.754 kb on - strand at 3558.754 kb on - strand at 3558.754 kb on - strand at 3558.756 kb on + strand at 3558.756 kb on + strand at 3558.756 kb on + strand at 3558.756 kb on + strand at 3558.756 kb on + strand at 3558.756 kb on + strand at 3558.756 kb on + strand at 3558.756 kb on - strand at 3558.759 kb on - strand at 3558.764 kb on - strand at 3558.764 kb on - strand at 3558.764 kb on - strand at 3558.764 kb on - strand at 3558.764 kb on - strand at 3558.764 kb on - strand at 3558.764 kb on - strand at 3558.764 kb on - strand at 3558.940 kb on + strand at 3558.989 kb on - strand at 3558.989 kb on - strand at 3559.005 kb on - strand at 3559.013 kb on - strand at 3559.016 kb on + strand at 3559.031 kb on + strand at 3559.034 kb on - strand at 3559.044 kb on + strand, within BWI76_RS17170 at 3559.053 kb on - strand, within BWI76_RS17170 at 3559.055 kb on + strand, within BWI76_RS17170 at 3559.087 kb on + strand, within BWI76_RS17170 at 3559.087 kb on + strand, within BWI76_RS17170 at 3559.087 kb on + strand, within BWI76_RS17170 at 3559.105 kb on - strand, within BWI76_RS17170 at 3559.105 kb on - strand, within BWI76_RS17170 at 3559.146 kb on + strand, within BWI76_RS17170
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL with sand remove 3,558,090 - BWI76_RS17155 0.57 -1.8 3,558,122 - BWI76_RS17155 0.72 -1.3 3,558,183 - +0.0 3,558,188 + +0.0 3,558,188 + +0.6 3,558,188 + -0.2 3,558,188 + +1.0 3,558,188 + -0.2 3,558,196 - +0.8 3,558,196 - -0.4 3,558,227 - -0.3 3,558,308 - BWI76_RS17160 0.38 -0.7 3,558,310 + BWI76_RS17160 0.39 -1.0 3,558,333 + BWI76_RS17160 0.50 -0.4 3,558,395 + BWI76_RS17160 0.78 -0.4 3,558,395 + BWI76_RS17160 0.78 -0.1 3,558,395 + BWI76_RS17160 0.78 -2.1 3,558,395 + BWI76_RS17160 0.78 +0.4 3,558,398 - BWI76_RS17160 0.79 +1.1 3,558,402 - BWI76_RS17160 0.81 +0.1 3,558,403 - BWI76_RS17160 0.82 +0.2 3,558,403 - BWI76_RS17160 0.82 +0.2 3,558,403 - BWI76_RS17160 0.82 +0.2 3,558,403 - BWI76_RS17160 0.82 +0.6 3,558,405 + BWI76_RS17160 0.83 +0.1 3,558,405 + BWI76_RS17160 0.83 +1.1 3,558,413 - BWI76_RS17160 0.86 +0.0 3,558,443 - -0.4 3,558,454 - -0.6 3,558,455 + -2.3 3,558,463 + +0.1 3,558,472 + +1.3 3,558,480 + +1.0 3,558,502 + BWI76_RS17165 0.19 +0.4 3,558,503 + BWI76_RS17165 0.20 +0.7 3,558,511 - BWI76_RS17165 0.23 +0.2 3,558,521 - BWI76_RS17165 0.27 +0.2 3,558,534 + BWI76_RS17165 0.33 -0.6 3,558,541 + -0.6 3,558,547 - BWI76_RS17165 0.38 -2.5 3,558,549 - BWI76_RS17165 0.39 +1.0 3,558,577 + BWI76_RS17165 0.51 +0.8 3,558,577 + BWI76_RS17165 0.51 +1.0 3,558,592 + BWI76_RS17165 0.58 -0.9 3,558,643 - BWI76_RS17165 0.79 +0.8 3,558,643 - BWI76_RS17165 0.79 -0.7 3,558,647 + BWI76_RS17165 0.81 -0.5 3,558,652 - BWI76_RS17165 0.83 -0.1 3,558,655 - BWI76_RS17165 0.85 +1.1 3,558,736 + -0.5 3,558,736 + -1.1 3,558,744 - -0.9 3,558,744 - -0.7 3,558,744 - -0.7 3,558,746 + +1.6 3,558,746 + -1.1 3,558,746 + -0.8 3,558,746 + +0.4 3,558,746 + -0.4 3,558,746 + +0.3 3,558,746 + +0.3 3,558,746 + +0.8 3,558,748 + +0.9 3,558,754 + +0.7 3,558,754 - -0.2 3,558,754 - +0.5 3,558,754 - -0.6 3,558,756 + +0.5 3,558,756 + +0.4 3,558,756 + -0.1 3,558,756 + -1.2 3,558,756 + +0.2 3,558,756 + +1.6 3,558,756 + +0.7 3,558,756 - +0.6 3,558,759 - +0.8 3,558,764 - -0.8 3,558,764 - -0.4 3,558,764 - +0.5 3,558,764 - +0.2 3,558,764 - -1.0 3,558,764 - +0.1 3,558,764 - +0.3 3,558,764 - -0.7 3,558,940 + -0.2 3,558,989 - -0.0 3,558,989 - -0.6 3,559,005 - +0.6 3,559,013 - +0.1 3,559,016 + -0.1 3,559,031 + +0.1 3,559,034 - -0.3 3,559,044 + BWI76_RS17170 0.11 +0.2 3,559,053 - BWI76_RS17170 0.12 -0.6 3,559,055 + BWI76_RS17170 0.12 -0.8 3,559,087 + BWI76_RS17170 0.17 +0.0 3,559,087 + BWI76_RS17170 0.17 -0.1 3,559,087 + BWI76_RS17170 0.17 -0.3 3,559,105 - BWI76_RS17170 0.19 +0.4 3,559,105 - BWI76_RS17170 0.19 +0.0 3,559,146 + BWI76_RS17170 0.24 -1.6
Or see this region's nucleotide sequence