Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16285

Experiment: LB in EcoFAB_3.5mL with sand

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16280 and BWI76_RS16285 are separated by 98 nucleotidesBWI76_RS16285 and BWI76_RS16290 are separated by 25 nucleotides BWI76_RS16280: BWI76_RS16280 - mannose-6-phosphate isomerase, at 3,378,527 to 3,379,705 _RS16280 BWI76_RS16285: BWI76_RS16285 - hypothetical protein, at 3,379,804 to 3,381,309 _RS16285 BWI76_RS16290: BWI76_RS16290 - Mal regulon transcriptional regulator MalI, at 3,381,335 to 3,382,363 _RS16290 Position (kb) 3379 3380 3381 3382Strain fitness (log2 ratio) -1 0 1at 3378.812 kb on + strand, within BWI76_RS16280at 3378.916 kb on + strand, within BWI76_RS16280at 3379.465 kb on - strand, within BWI76_RS16280at 3379.537 kb on + strand, within BWI76_RS16280at 3379.933 kb on + strandat 3380.154 kb on + strand, within BWI76_RS16285at 3380.167 kb on - strand, within BWI76_RS16285at 3380.562 kb on + strand, within BWI76_RS16285at 3380.622 kb on - strand, within BWI76_RS16285at 3380.837 kb on - strand, within BWI76_RS16285at 3380.850 kb on + strand, within BWI76_RS16285at 3381.054 kb on - strand, within BWI76_RS16285at 3381.077 kb on - strand, within BWI76_RS16285at 3381.426 kb on - strandat 3381.444 kb on - strand, within BWI76_RS16290at 3381.494 kb on - strand, within BWI76_RS16290at 3381.580 kb on + strand, within BWI76_RS16290at 3382.293 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL with sand
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3,378,812 + BWI76_RS16280 0.24 -0.3
3,378,916 + BWI76_RS16280 0.33 -1.2
3,379,465 - BWI76_RS16280 0.80 +0.3
3,379,537 + BWI76_RS16280 0.86 +0.4
3,379,933 + -0.1
3,380,154 + BWI76_RS16285 0.23 +1.5
3,380,167 - BWI76_RS16285 0.24 +0.2
3,380,562 + BWI76_RS16285 0.50 +0.6
3,380,622 - BWI76_RS16285 0.54 +0.8
3,380,837 - BWI76_RS16285 0.69 +0.8
3,380,850 + BWI76_RS16285 0.69 +0.6
3,381,054 - BWI76_RS16285 0.83 +0.3
3,381,077 - BWI76_RS16285 0.85 +0.7
3,381,426 - -0.9
3,381,444 - BWI76_RS16290 0.11 -0.3
3,381,494 - BWI76_RS16290 0.15 -0.7
3,381,580 + BWI76_RS16290 0.24 -0.1
3,382,293 - -0.5

Or see this region's nucleotide sequence