Experiment: LB in EcoFAB_3.5mL with sand
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06940 and BWI76_RS06945 are separated by 28 nucleotides BWI76_RS06945 and BWI76_RS06950 overlap by 63 nucleotides BWI76_RS06950 and BWI76_RS06955 overlap by 4 nucleotides
BWI76_RS06940: BWI76_RS06940 - short-chain dehydrogenase/reductase, at 1,457,557 to 1,458,327
_RS06940
BWI76_RS06945: BWI76_RS06945 - multifunctional acyl-CoA thioesterase I/protease I/lysophospholipase L1, at 1,458,356 to 1,459,012
_RS06945
BWI76_RS06950: BWI76_RS06950 - ABC transporter ATP-binding protein, at 1,458,950 to 1,459,636
_RS06950
BWI76_RS06955: BWI76_RS06955 - sugar ABC transporter permease, at 1,459,633 to 1,462,050
_RS06955
Position (kb)
1458
1459
1460 Strain fitness (log2 ratio)
-1
0
1 at 1458.251 kb on - strand at 1458.371 kb on + strand at 1458.371 kb on + strand at 1458.523 kb on + strand, within BWI76_RS06945 at 1458.523 kb on + strand, within BWI76_RS06945 at 1458.550 kb on + strand, within BWI76_RS06945 at 1458.679 kb on + strand, within BWI76_RS06945 at 1458.819 kb on + strand, within BWI76_RS06945 at 1458.827 kb on - strand, within BWI76_RS06945 at 1459.254 kb on + strand, within BWI76_RS06950 at 1459.345 kb on - strand, within BWI76_RS06950 at 1459.445 kb on + strand, within BWI76_RS06950 at 1459.816 kb on - strand at 1459.821 kb on - strand at 1459.849 kb on + strand at 1459.993 kb on - strand, within BWI76_RS06955 at 1460.075 kb on + strand, within BWI76_RS06955 at 1460.075 kb on + strand, within BWI76_RS06955 at 1460.123 kb on + strand, within BWI76_RS06955 at 1460.321 kb on + strand, within BWI76_RS06955
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL with sand remove 1,458,251 - +0.3 1,458,371 + +0.2 1,458,371 + -0.1 1,458,523 + BWI76_RS06945 0.25 +0.0 1,458,523 + BWI76_RS06945 0.25 +0.8 1,458,550 + BWI76_RS06945 0.30 -0.5 1,458,679 + BWI76_RS06945 0.49 -0.3 1,458,819 + BWI76_RS06945 0.70 +0.3 1,458,827 - BWI76_RS06945 0.72 +0.8 1,459,254 + BWI76_RS06950 0.44 +0.1 1,459,345 - BWI76_RS06950 0.57 -1.7 1,459,445 + BWI76_RS06950 0.72 -0.7 1,459,816 - -0.3 1,459,821 - +0.4 1,459,849 + -0.4 1,459,993 - BWI76_RS06955 0.15 -0.0 1,460,075 + BWI76_RS06955 0.18 +0.1 1,460,075 + BWI76_RS06955 0.18 +0.6 1,460,123 + BWI76_RS06955 0.20 +0.5 1,460,321 + BWI76_RS06955 0.28 +0.5
Or see this region's nucleotide sequence