Experiment: LB in EcoFAB_3.5mL with sand
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03160 and BWI76_RS03165 are separated by 768 nucleotides BWI76_RS03165 and BWI76_RS03170 are separated by 17 nucleotides BWI76_RS03170 and BWI76_RS03175 overlap by 4 nucleotides
BWI76_RS03160: BWI76_RS03160 - hypothetical protein, at 651,430 to 653,463
_RS03160
BWI76_RS03165: BWI76_RS03165 - phage polarity suppression protein, at 654,232 to 654,798
_RS03165
BWI76_RS03170: BWI76_RS03170 - hypothetical protein, at 654,816 to 655,061
_RS03170
BWI76_RS03175: BWI76_RS03175 - hypothetical protein, at 655,058 to 655,795
_RS03175
Position (kb) 654
655 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 653.265 kb on + strand at 653.276 kb on + strand at 653.403 kb on + strand at 653.456 kb on + strand at 653.456 kb on + strand at 653.509 kb on - strand at 653.509 kb on - strand at 653.621 kb on + strand at 653.621 kb on + strand at 653.621 kb on + strand at 653.621 kb on + strand at 653.621 kb on + strand at 653.621 kb on + strand at 653.621 kb on + strand at 653.626 kb on + strand at 653.626 kb on + strand at 653.626 kb on + strand at 653.626 kb on + strand at 653.626 kb on + strand at 653.626 kb on + strand at 653.626 kb on + strand at 653.626 kb on + strand at 653.629 kb on - strand at 653.629 kb on - strand at 653.659 kb on + strand at 653.664 kb on + strand at 653.667 kb on + strand at 653.667 kb on + strand at 653.674 kb on + strand at 653.674 kb on + strand at 653.682 kb on - strand at 653.735 kb on - strand at 653.736 kb on + strand at 653.736 kb on + strand at 653.736 kb on + strand at 653.736 kb on + strand at 653.736 kb on + strand at 653.736 kb on + strand at 653.736 kb on + strand at 653.744 kb on - strand at 653.744 kb on - strand at 653.744 kb on - strand at 653.744 kb on - strand at 653.744 kb on - strand at 653.744 kb on - strand at 653.744 kb on - strand at 653.749 kb on - strand at 653.770 kb on + strand at 653.770 kb on + strand at 653.778 kb on + strand at 653.782 kb on + strand at 653.782 kb on + strand at 653.782 kb on + strand at 653.782 kb on + strand at 653.782 kb on + strand at 653.782 kb on + strand at 653.782 kb on + strand at 653.782 kb on + strand at 653.783 kb on + strand at 653.783 kb on + strand at 653.783 kb on + strand at 653.788 kb on + strand at 653.788 kb on + strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.790 kb on - strand at 653.791 kb on - strand at 653.791 kb on - strand at 653.792 kb on + strand at 653.793 kb on + strand at 653.793 kb on + strand at 653.793 kb on + strand at 653.793 kb on + strand at 653.793 kb on + strand at 653.793 kb on + strand at 653.793 kb on + strand at 653.793 kb on + strand at 653.796 kb on - strand at 653.796 kb on - strand at 653.798 kb on + strand at 653.801 kb on - strand at 653.801 kb on - strand at 653.808 kb on - strand at 653.871 kb on + strand at 653.878 kb on + strand at 653.878 kb on + strand at 653.878 kb on - strand at 653.881 kb on + strand at 653.889 kb on - strand at 653.889 kb on - strand at 653.892 kb on + strand at 653.892 kb on + strand at 653.892 kb on + strand at 653.892 kb on + strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.905 kb on - strand at 653.907 kb on + strand at 653.907 kb on + strand at 653.914 kb on + strand at 653.915 kb on - strand at 653.915 kb on - strand at 653.915 kb on - strand at 653.922 kb on - strand at 653.923 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.943 kb on - strand at 653.943 kb on - strand at 653.943 kb on - strand at 653.943 kb on - strand at 653.946 kb on - strand at 653.948 kb on - strand at 653.948 kb on - strand at 653.956 kb on - strand at 653.972 kb on - strand at 653.977 kb on - strand at 653.982 kb on - strand at 653.982 kb on - strand at 653.982 kb on - strand at 653.982 kb on - strand at 654.249 kb on - strand at 654.249 kb on - strand at 654.321 kb on + strand, within BWI76_RS03165 at 654.416 kb on - strand, within BWI76_RS03165 at 654.420 kb on + strand, within BWI76_RS03165 at 654.436 kb on + strand, within BWI76_RS03165 at 654.518 kb on - strand, within BWI76_RS03165 at 654.536 kb on - strand, within BWI76_RS03165 at 654.663 kb on + strand, within BWI76_RS03165 at 654.663 kb on + strand, within BWI76_RS03165 at 654.663 kb on + strand, within BWI76_RS03165 at 654.671 kb on - strand, within BWI76_RS03165 at 654.671 kb on - strand, within BWI76_RS03165 at 654.674 kb on + strand, within BWI76_RS03165 at 654.682 kb on - strand, within BWI76_RS03165 at 654.807 kb on - strand at 654.843 kb on + strand, within BWI76_RS03170 at 654.932 kb on + strand, within BWI76_RS03170 at 654.940 kb on - strand, within BWI76_RS03170 at 654.940 kb on - strand, within BWI76_RS03170 at 655.324 kb on + strand, within BWI76_RS03175 at 655.344 kb on - strand, within BWI76_RS03175 at 655.377 kb on - strand, within BWI76_RS03175 at 655.421 kb on - strand, within BWI76_RS03175 at 655.481 kb on - strand, within BWI76_RS03175 at 655.714 kb on + strand, within BWI76_RS03175 at 655.714 kb on + strand, within BWI76_RS03175 at 655.733 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL with sand remove 653,265 + -1.5 653,276 + -1.7 653,403 + +0.7 653,456 + +0.9 653,456 + +0.8 653,509 - -0.8 653,509 - -0.5 653,621 + -0.5 653,621 + -0.4 653,621 + +0.5 653,621 + -0.9 653,621 + +0.1 653,621 + -0.7 653,621 + -0.5 653,626 + +0.2 653,626 + -0.2 653,626 + +0.1 653,626 + -0.3 653,626 + -0.3 653,626 + +2.0 653,626 + +0.3 653,626 + +1.1 653,629 - -1.4 653,629 - -0.3 653,659 + -0.5 653,664 + -0.1 653,667 + -1.6 653,667 + +0.2 653,674 + -0.5 653,674 + +0.2 653,682 - +1.0 653,735 - +0.4 653,736 + -1.1 653,736 + -2.0 653,736 + -0.3 653,736 + -0.6 653,736 + -0.4 653,736 + +0.0 653,736 + -0.1 653,744 - -1.4 653,744 - -1.0 653,744 - -0.5 653,744 - -0.5 653,744 - +0.3 653,744 - +0.6 653,744 - -0.4 653,749 - -0.5 653,770 + +0.1 653,770 + -0.0 653,778 + -0.7 653,782 + +0.5 653,782 + +0.6 653,782 + +1.6 653,782 + +0.6 653,782 + +0.8 653,782 + -1.0 653,782 + +0.1 653,782 + +1.1 653,783 + -0.1 653,783 + +0.4 653,783 + -1.3 653,788 + -0.1 653,788 + +0.6 653,790 - -1.3 653,790 - -0.4 653,790 - -0.9 653,790 - -0.1 653,790 - -0.8 653,790 - -0.5 653,790 - -0.7 653,790 - +0.5 653,790 - +0.0 653,790 - -0.5 653,790 - -2.5 653,791 - -2.5 653,791 - -0.3 653,792 + -0.0 653,793 + -0.0 653,793 + -0.5 653,793 + -0.1 653,793 + +0.1 653,793 + +1.1 653,793 + -0.8 653,793 + +0.1 653,793 + +0.3 653,796 - -0.0 653,796 - +1.2 653,798 + -0.6 653,801 - -1.0 653,801 - -1.5 653,808 - -0.4 653,871 + +0.1 653,878 + +0.6 653,878 + +1.8 653,878 - +1.1 653,881 + +1.1 653,889 - -0.7 653,889 - +0.1 653,892 + +1.7 653,892 + -0.4 653,892 + +0.7 653,892 + +0.6 653,900 - -0.4 653,900 - +0.2 653,900 - -3.2 653,900 - +0.1 653,900 - +0.2 653,900 - +0.3 653,900 - -0.1 653,905 - -0.1 653,907 + -1.7 653,907 + -1.5 653,914 + -1.0 653,915 - -0.0 653,915 - -0.9 653,915 - +0.4 653,922 - -1.2 653,923 + -0.1 653,925 + +0.0 653,925 + +0.1 653,925 + +0.1 653,925 + -0.4 653,925 + -1.3 653,925 + -0.4 653,925 + -0.2 653,925 + -0.2 653,925 + -1.9 653,925 + +0.4 653,925 + +0.9 653,933 - +0.8 653,933 - +0.5 653,933 - +0.5 653,933 - -3.0 653,933 - -0.5 653,933 - +1.1 653,933 - +0.3 653,933 - -0.1 653,933 - +0.2 653,933 - -0.5 653,933 - +0.9 653,933 - -0.1 653,933 - +0.5 653,933 - -0.4 653,943 - +0.4 653,943 - +0.2 653,943 - +0.5 653,943 - -0.9 653,946 - +0.3 653,948 - +1.3 653,948 - +1.1 653,956 - -0.8 653,972 - +0.2 653,977 - -0.3 653,982 - -0.1 653,982 - +0.3 653,982 - -0.4 653,982 - -0.4 654,249 - +0.4 654,249 - -1.1 654,321 + BWI76_RS03165 0.16 +0.2 654,416 - BWI76_RS03165 0.32 +0.9 654,420 + BWI76_RS03165 0.33 +0.0 654,436 + BWI76_RS03165 0.36 +1.1 654,518 - BWI76_RS03165 0.50 -0.4 654,536 - BWI76_RS03165 0.54 -0.0 654,663 + BWI76_RS03165 0.76 +0.9 654,663 + BWI76_RS03165 0.76 +0.4 654,663 + BWI76_RS03165 0.76 +1.4 654,671 - BWI76_RS03165 0.77 +1.1 654,671 - BWI76_RS03165 0.77 +0.1 654,674 + BWI76_RS03165 0.78 +0.3 654,682 - BWI76_RS03165 0.79 +0.7 654,807 - -0.1 654,843 + BWI76_RS03170 0.11 +0.1 654,932 + BWI76_RS03170 0.47 -0.1 654,940 - BWI76_RS03170 0.50 -0.8 654,940 - BWI76_RS03170 0.50 -0.5 655,324 + BWI76_RS03175 0.36 -0.6 655,344 - BWI76_RS03175 0.39 -0.3 655,377 - BWI76_RS03175 0.43 -0.1 655,421 - BWI76_RS03175 0.49 +0.1 655,481 - BWI76_RS03175 0.57 +0.2 655,714 + BWI76_RS03175 0.89 +0.6 655,714 + BWI76_RS03175 0.89 -0.2 655,733 + +0.3
Or see this region's nucleotide sequence