Experiment: LB in EcoFAB_3.5mL with sand
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01710 and BWI76_RS01715 are separated by 31 nucleotides BWI76_RS01715 and BWI76_RS01720 overlap by 8 nucleotides BWI76_RS01720 and BWI76_RS01725 are separated by 123 nucleotides BWI76_RS01725 and BWI76_RS01730 are separated by 11 nucleotides
BWI76_RS01710: BWI76_RS01710 - DNA gyrase inhibitor, at 351,356 to 351,673
_RS01710
BWI76_RS01715: BWI76_RS01715 - shikimate 5-dehydrogenase, at 351,705 to 352,514
_RS01715
BWI76_RS01720: BWI76_RS01720 - L-sorbose 1-phosphate reductase (EC 1.1.1.-) <small>(from data)</small>, at 352,507 to 353,736
_RS01720
BWI76_RS01725: BWI76_RS01725 - PTS mannose/fructose/sorbose family transporter subunit IID, at 353,860 to 354,684
_RS01725
BWI76_RS01730: BWI76_RS01730 - PTS mannose/fructose/sorbose transporter subunit IIC, at 354,696 to 355,493
_RS01730
Position (kb) 352
353
354 Strain fitness (log2 ratio) -2
-1
0
1 at 351.622 kb on + strand, within BWI76_RS01710 at 351.622 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.627 kb on + strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.630 kb on - strand, within BWI76_RS01710 at 351.635 kb on - strand, within BWI76_RS01710 at 351.682 kb on + strand at 351.682 kb on + strand at 351.690 kb on - strand at 351.722 kb on - strand at 351.777 kb on + strand at 351.834 kb on + strand, within BWI76_RS01715 at 351.988 kb on - strand, within BWI76_RS01715 at 351.999 kb on - strand, within BWI76_RS01715 at 352.145 kb on - strand, within BWI76_RS01715 at 352.218 kb on - strand, within BWI76_RS01715 at 352.249 kb on + strand, within BWI76_RS01715 at 352.280 kb on + strand, within BWI76_RS01715 at 352.309 kb on - strand, within BWI76_RS01715 at 352.346 kb on - strand, within BWI76_RS01715 at 352.391 kb on - strand, within BWI76_RS01715 at 352.392 kb on + strand, within BWI76_RS01715 at 352.416 kb on - strand, within BWI76_RS01715 at 352.486 kb on + strand at 352.687 kb on + strand, within BWI76_RS01720 at 352.722 kb on + strand, within BWI76_RS01720 at 352.724 kb on - strand, within BWI76_RS01720 at 352.930 kb on + strand, within BWI76_RS01720 at 353.173 kb on + strand, within BWI76_RS01720 at 353.189 kb on + strand, within BWI76_RS01720 at 353.450 kb on - strand, within BWI76_RS01720 at 353.467 kb on + strand, within BWI76_RS01720 at 353.467 kb on + strand, within BWI76_RS01720 at 353.721 kb on + strand at 353.748 kb on + strand at 353.815 kb on + strand at 353.942 kb on - strand at 353.951 kb on + strand, within BWI76_RS01725 at 353.978 kb on - strand, within BWI76_RS01725 at 353.985 kb on + strand, within BWI76_RS01725 at 353.990 kb on + strand, within BWI76_RS01725 at 354.077 kb on - strand, within BWI76_RS01725 at 354.134 kb on - strand, within BWI76_RS01725 at 354.182 kb on + strand, within BWI76_RS01725 at 354.223 kb on + strand, within BWI76_RS01725 at 354.335 kb on + strand, within BWI76_RS01725 at 354.477 kb on + strand, within BWI76_RS01725 at 354.718 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL with sand remove 351,622 + BWI76_RS01710 0.84 +0.7 351,622 + BWI76_RS01710 0.84 +0.8 351,627 + BWI76_RS01710 0.85 +0.6 351,627 + BWI76_RS01710 0.85 +0.6 351,627 + BWI76_RS01710 0.85 -1.1 351,630 - BWI76_RS01710 0.86 -0.7 351,630 - BWI76_RS01710 0.86 +0.6 351,630 - BWI76_RS01710 0.86 -0.4 351,635 - BWI76_RS01710 0.88 +0.1 351,682 + +0.1 351,682 + -0.7 351,690 - -0.3 351,722 - -1.2 351,777 + -0.4 351,834 + BWI76_RS01715 0.16 -0.5 351,988 - BWI76_RS01715 0.35 -0.3 351,999 - BWI76_RS01715 0.36 -1.0 352,145 - BWI76_RS01715 0.54 -0.4 352,218 - BWI76_RS01715 0.63 +0.1 352,249 + BWI76_RS01715 0.67 +0.6 352,280 + BWI76_RS01715 0.71 +0.7 352,309 - BWI76_RS01715 0.75 -0.6 352,346 - BWI76_RS01715 0.79 -0.9 352,391 - BWI76_RS01715 0.85 +0.5 352,392 + BWI76_RS01715 0.85 -0.9 352,416 - BWI76_RS01715 0.88 -1.6 352,486 + +0.2 352,687 + BWI76_RS01720 0.15 +0.2 352,722 + BWI76_RS01720 0.17 -1.0 352,724 - BWI76_RS01720 0.18 -1.6 352,930 + BWI76_RS01720 0.34 -1.5 353,173 + BWI76_RS01720 0.54 -0.1 353,189 + BWI76_RS01720 0.55 -0.3 353,450 - BWI76_RS01720 0.77 -1.2 353,467 + BWI76_RS01720 0.78 -1.0 353,467 + BWI76_RS01720 0.78 -1.0 353,721 + +0.5 353,748 + +0.4 353,815 + +0.2 353,942 - -1.4 353,951 + BWI76_RS01725 0.11 +0.4 353,978 - BWI76_RS01725 0.14 +0.9 353,985 + BWI76_RS01725 0.15 +0.3 353,990 + BWI76_RS01725 0.16 -1.4 354,077 - BWI76_RS01725 0.26 -0.5 354,134 - BWI76_RS01725 0.33 -0.3 354,182 + BWI76_RS01725 0.39 +0.2 354,223 + BWI76_RS01725 0.44 -2.3 354,335 + BWI76_RS01725 0.58 -0.9 354,477 + BWI76_RS01725 0.75 +1.3 354,718 - +0.3
Or see this region's nucleotide sequence