Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00250

Experiment: LB in EcoFAB_3.5mL with sand

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00240 and BWI76_RS00245 are separated by 91 nucleotidesBWI76_RS00245 and BWI76_RS00250 are separated by 152 nucleotidesBWI76_RS00250 and BWI76_RS00255 are separated by 7 nucleotides BWI76_RS00240: BWI76_RS00240 - FMN-binding protein MioC, at 48,952 to 49,392 _RS00240 BWI76_RS00245: BWI76_RS00245 - transcriptional regulator AsnC, at 49,484 to 49,942 _RS00245 BWI76_RS00250: BWI76_RS00250 - asparagine synthetase A, at 50,095 to 51,087 _RS00250 BWI76_RS00255: BWI76_RS00255 - hypothetical protein, at 51,095 to 52,543 _RS00255 Position (kb) 50 51 52Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 49.102 kb on + strand, within BWI76_RS00240at 49.227 kb on - strand, within BWI76_RS00240at 49.287 kb on + strandat 49.310 kb on + strandat 49.354 kb on + strandat 49.357 kb on - strandat 49.362 kb on - strandat 49.367 kb on - strandat 49.384 kb on - strandat 49.385 kb on - strandat 49.409 kb on + strandat 49.417 kb on - strandat 49.417 kb on - strandat 49.417 kb on - strandat 49.422 kb on - strandat 49.431 kb on - strandat 49.431 kb on - strandat 49.576 kb on + strand, within BWI76_RS00245at 49.641 kb on + strand, within BWI76_RS00245at 49.666 kb on - strand, within BWI76_RS00245at 49.677 kb on + strand, within BWI76_RS00245at 49.677 kb on + strand, within BWI76_RS00245at 49.677 kb on + strand, within BWI76_RS00245at 49.677 kb on + strand, within BWI76_RS00245at 49.677 kb on + strand, within BWI76_RS00245at 49.682 kb on + strand, within BWI76_RS00245at 49.685 kb on - strand, within BWI76_RS00245at 49.687 kb on + strand, within BWI76_RS00245at 49.743 kb on + strand, within BWI76_RS00245at 49.743 kb on + strand, within BWI76_RS00245at 49.743 kb on + strand, within BWI76_RS00245at 49.756 kb on + strand, within BWI76_RS00245at 49.764 kb on - strand, within BWI76_RS00245at 49.766 kb on + strand, within BWI76_RS00245at 49.822 kb on + strand, within BWI76_RS00245at 49.830 kb on + strand, within BWI76_RS00245at 49.830 kb on - strand, within BWI76_RS00245at 49.830 kb on - strand, within BWI76_RS00245at 49.845 kb on - strand, within BWI76_RS00245at 49.861 kb on + strand, within BWI76_RS00245at 49.869 kb on - strand, within BWI76_RS00245at 49.895 kb on + strand, within BWI76_RS00245at 49.896 kb on + strand, within BWI76_RS00245at 49.897 kb on + strandat 49.903 kb on - strandat 50.111 kb on + strandat 50.158 kb on - strandat 50.227 kb on + strand, within BWI76_RS00250at 50.237 kb on - strand, within BWI76_RS00250at 50.300 kb on - strand, within BWI76_RS00250at 50.335 kb on - strand, within BWI76_RS00250at 50.346 kb on + strand, within BWI76_RS00250at 50.367 kb on + strand, within BWI76_RS00250at 50.387 kb on - strand, within BWI76_RS00250at 50.398 kb on + strand, within BWI76_RS00250at 50.440 kb on - strand, within BWI76_RS00250at 50.445 kb on - strand, within BWI76_RS00250at 50.450 kb on - strand, within BWI76_RS00250at 50.454 kb on - strand, within BWI76_RS00250at 50.604 kb on - strand, within BWI76_RS00250at 50.839 kb on - strand, within BWI76_RS00250at 50.936 kb on - strand, within BWI76_RS00250at 50.975 kb on + strand, within BWI76_RS00250at 50.975 kb on + strand, within BWI76_RS00250at 51.015 kb on + strandat 51.301 kb on + strand, within BWI76_RS00255at 51.353 kb on - strand, within BWI76_RS00255at 51.516 kb on + strand, within BWI76_RS00255at 51.543 kb on - strand, within BWI76_RS00255at 51.672 kb on + strand, within BWI76_RS00255at 51.786 kb on - strand, within BWI76_RS00255at 51.893 kb on - strand, within BWI76_RS00255at 51.912 kb on + strand, within BWI76_RS00255at 52.043 kb on - strand, within BWI76_RS00255at 52.043 kb on - strand, within BWI76_RS00255at 52.054 kb on + strand, within BWI76_RS00255at 52.064 kb on + strand, within BWI76_RS00255

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Per-strain Table

Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL with sand
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49,102 + BWI76_RS00240 0.34 +1.2
49,227 - BWI76_RS00240 0.62 -0.3
49,287 + +2.2
49,310 + -1.0
49,354 + -0.8
49,357 - -1.7
49,362 - -1.1
49,367 - -1.4
49,384 - -0.0
49,385 - -1.7
49,409 + -0.9
49,417 - -0.3
49,417 - +0.2
49,417 - +0.5
49,422 - -1.3
49,431 - -0.6
49,431 - -0.6
49,576 + BWI76_RS00245 0.20 +0.3
49,641 + BWI76_RS00245 0.34 +0.6
49,666 - BWI76_RS00245 0.40 +0.2
49,677 + BWI76_RS00245 0.42 +0.7
49,677 + BWI76_RS00245 0.42 -0.1
49,677 + BWI76_RS00245 0.42 +0.1
49,677 + BWI76_RS00245 0.42 +0.2
49,677 + BWI76_RS00245 0.42 -0.6
49,682 + BWI76_RS00245 0.43 +0.3
49,685 - BWI76_RS00245 0.44 -3.0
49,687 + BWI76_RS00245 0.44 +0.3
49,743 + BWI76_RS00245 0.56 -0.4
49,743 + BWI76_RS00245 0.56 -0.8
49,743 + BWI76_RS00245 0.56 -0.6
49,756 + BWI76_RS00245 0.59 +0.0
49,764 - BWI76_RS00245 0.61 -0.1
49,766 + BWI76_RS00245 0.61 -0.0
49,822 + BWI76_RS00245 0.74 +0.5
49,830 + BWI76_RS00245 0.75 -2.2
49,830 - BWI76_RS00245 0.75 -1.4
49,830 - BWI76_RS00245 0.75 +2.3
49,845 - BWI76_RS00245 0.79 +0.5
49,861 + BWI76_RS00245 0.82 -0.5
49,869 - BWI76_RS00245 0.84 -0.2
49,895 + BWI76_RS00245 0.90 +0.5
49,896 + BWI76_RS00245 0.90 -0.1
49,897 + +0.7
49,903 - -0.6
50,111 + +0.3
50,158 - -0.8
50,227 + BWI76_RS00250 0.13 -0.3
50,237 - BWI76_RS00250 0.14 -1.0
50,300 - BWI76_RS00250 0.21 +0.2
50,335 - BWI76_RS00250 0.24 +0.6
50,346 + BWI76_RS00250 0.25 +0.3
50,367 + BWI76_RS00250 0.27 -1.1
50,387 - BWI76_RS00250 0.29 -1.4
50,398 + BWI76_RS00250 0.31 -0.5
50,440 - BWI76_RS00250 0.35 -0.3
50,445 - BWI76_RS00250 0.35 +0.5
50,450 - BWI76_RS00250 0.36 -0.0
50,454 - BWI76_RS00250 0.36 -0.9
50,604 - BWI76_RS00250 0.51 +0.4
50,839 - BWI76_RS00250 0.75 -0.1
50,936 - BWI76_RS00250 0.85 -0.3
50,975 + BWI76_RS00250 0.89 +0.4
50,975 + BWI76_RS00250 0.89 -0.4
51,015 + +1.1
51,301 + BWI76_RS00255 0.14 -1.4
51,353 - BWI76_RS00255 0.18 +0.7
51,516 + BWI76_RS00255 0.29 -0.1
51,543 - BWI76_RS00255 0.31 -0.6
51,672 + BWI76_RS00255 0.40 -0.2
51,786 - BWI76_RS00255 0.48 +0.1
51,893 - BWI76_RS00255 0.55 -0.1
51,912 + BWI76_RS00255 0.56 -0.6
52,043 - BWI76_RS00255 0.65 -0.8
52,043 - BWI76_RS00255 0.65 +1.3
52,054 + BWI76_RS00255 0.66 +0.1
52,064 + BWI76_RS00255 0.67 -0.5

Or see this region's nucleotide sequence