Experiment: Biofilm in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxJ and Synpcc7942_1195 are separated by 225 nucleotides Synpcc7942_1195 and sipA are separated by 76 nucleotides sipA and trpC are separated by 306 nucleotides
Synpcc7942_1194: pdxJ - pyridoxal phosphate biosynthetic protein PdxJ, at 1,222,698 to 1,223,495
pdxJ
Synpcc7942_1195: Synpcc7942_1195 - hypothetical protein, at 1,223,721 to 1,224,221
_1195
Synpcc7942_1196: sipA - hypothetical protein, at 1,224,298 to 1,224,534
sipA
Synpcc7942_1197: trpC - indole-3-glycerol-phosphate synthase, at 1,224,841 to 1,225,728
trpC
Position (kb)
1224
1225 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1223.489 kb on + strand at 1223.489 kb on + strand at 1223.489 kb on + strand at 1223.490 kb on + strand at 1223.490 kb on + strand at 1223.492 kb on - strand at 1223.497 kb on - strand at 1223.500 kb on + strand at 1223.509 kb on + strand at 1223.538 kb on + strand at 1223.552 kb on - strand at 1223.584 kb on + strand at 1223.599 kb on - strand at 1223.608 kb on - strand at 1223.646 kb on - strand at 1223.679 kb on - strand at 1223.713 kb on - strand at 1223.718 kb on + strand at 1223.728 kb on + strand at 1223.736 kb on - strand at 1223.736 kb on - strand at 1223.737 kb on + strand at 1223.742 kb on + strand at 1223.742 kb on + strand at 1223.742 kb on + strand at 1223.750 kb on - strand at 1223.773 kb on + strand, within Synpcc7942_1195 at 1223.775 kb on - strand, within Synpcc7942_1195 at 1223.775 kb on - strand, within Synpcc7942_1195 at 1223.807 kb on - strand, within Synpcc7942_1195 at 1223.820 kb on - strand, within Synpcc7942_1195 at 1223.837 kb on - strand, within Synpcc7942_1195 at 1223.850 kb on - strand at 1223.855 kb on - strand, within Synpcc7942_1195 at 1223.863 kb on - strand, within Synpcc7942_1195 at 1223.865 kb on + strand, within Synpcc7942_1195 at 1223.873 kb on - strand, within Synpcc7942_1195 at 1223.883 kb on - strand, within Synpcc7942_1195 at 1223.898 kb on + strand, within Synpcc7942_1195 at 1223.912 kb on - strand, within Synpcc7942_1195 at 1223.948 kb on - strand, within Synpcc7942_1195 at 1223.966 kb on + strand, within Synpcc7942_1195 at 1223.982 kb on + strand, within Synpcc7942_1195 at 1223.982 kb on + strand, within Synpcc7942_1195 at 1223.989 kb on + strand, within Synpcc7942_1195 at 1223.990 kb on - strand, within Synpcc7942_1195 at 1224.019 kb on + strand, within Synpcc7942_1195 at 1224.026 kb on + strand, within Synpcc7942_1195 at 1224.026 kb on + strand, within Synpcc7942_1195 at 1224.034 kb on - strand, within Synpcc7942_1195 at 1224.036 kb on - strand, within Synpcc7942_1195 at 1224.036 kb on - strand, within Synpcc7942_1195 at 1224.076 kb on - strand, within Synpcc7942_1195 at 1224.086 kb on - strand, within Synpcc7942_1195 at 1224.111 kb on - strand, within Synpcc7942_1195 at 1224.111 kb on - strand, within Synpcc7942_1195 at 1224.145 kb on + strand, within Synpcc7942_1195 at 1224.167 kb on + strand, within Synpcc7942_1195 at 1224.279 kb on + strand at 1224.279 kb on + strand at 1224.279 kb on + strand at 1224.280 kb on - strand at 1224.287 kb on - strand at 1224.287 kb on - strand at 1224.292 kb on - strand at 1224.292 kb on - strand at 1224.432 kb on + strand, within sipA at 1224.443 kb on + strand, within sipA at 1224.454 kb on - strand, within sipA at 1224.475 kb on - strand, within sipA at 1224.475 kb on - strand, within sipA at 1224.495 kb on + strand, within sipA at 1224.529 kb on + strand at 1224.529 kb on + strand at 1224.530 kb on + strand at 1224.537 kb on - strand at 1224.537 kb on - strand at 1224.559 kb on - strand at 1224.561 kb on + strand at 1224.587 kb on - strand at 1224.593 kb on - strand at 1224.622 kb on - strand at 1224.623 kb on - strand at 1224.630 kb on - strand at 1224.663 kb on - strand at 1224.683 kb on - strand at 1224.684 kb on - strand at 1224.712 kb on + strand at 1224.716 kb on + strand at 1224.721 kb on + strand at 1224.753 kb on - strand at 1224.753 kb on - strand at 1224.754 kb on - strand at 1224.772 kb on + strand at 1224.772 kb on + strand at 1224.772 kb on + strand at 1224.792 kb on + strand at 1224.839 kb on + strand at 1224.839 kb on + strand at 1224.842 kb on - strand at 1224.846 kb on - strand at 1224.846 kb on - strand at 1224.847 kb on - strand at 1224.847 kb on - strand at 1224.853 kb on - strand at 1224.871 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in WT Conditioned Media in Test Tubes with Spin rep 1 remove 1,223,489 + -1.4 1,223,489 + -1.0 1,223,489 + +3.6 1,223,490 + +1.9 1,223,490 + +0.5 1,223,492 - +1.0 1,223,497 - -1.0 1,223,500 + +0.3 1,223,509 + +0.2 1,223,538 + -0.2 1,223,552 - +0.0 1,223,584 + +0.0 1,223,599 - -0.7 1,223,608 - +0.7 1,223,646 - +1.5 1,223,679 - +2.0 1,223,713 - -1.1 1,223,718 + +0.0 1,223,728 + -1.0 1,223,736 - -1.3 1,223,736 - -0.7 1,223,737 + +0.0 1,223,742 + -0.3 1,223,742 + -0.1 1,223,742 + -0.6 1,223,750 - -0.5 1,223,773 + Synpcc7942_1195 0.10 -0.5 1,223,775 - Synpcc7942_1195 0.11 +1.0 1,223,775 - Synpcc7942_1195 0.11 +1.8 1,223,807 - Synpcc7942_1195 0.17 -1.3 1,223,820 - Synpcc7942_1195 0.20 -0.6 1,223,837 - Synpcc7942_1195 0.23 +2.4 1,223,850 - -0.8 1,223,855 - Synpcc7942_1195 0.27 +1.8 1,223,863 - Synpcc7942_1195 0.28 -0.4 1,223,865 + Synpcc7942_1195 0.29 -1.7 1,223,873 - Synpcc7942_1195 0.30 -1.2 1,223,883 - Synpcc7942_1195 0.32 +0.8 1,223,898 + Synpcc7942_1195 0.35 -1.5 1,223,912 - Synpcc7942_1195 0.38 +2.1 1,223,948 - Synpcc7942_1195 0.45 +2.9 1,223,966 + Synpcc7942_1195 0.49 -0.6 1,223,982 + Synpcc7942_1195 0.52 +0.7 1,223,982 + Synpcc7942_1195 0.52 -0.2 1,223,989 + Synpcc7942_1195 0.53 +0.6 1,223,990 - Synpcc7942_1195 0.54 -1.6 1,224,019 + Synpcc7942_1195 0.59 -0.6 1,224,026 + Synpcc7942_1195 0.61 -1.0 1,224,026 + Synpcc7942_1195 0.61 -0.1 1,224,034 - Synpcc7942_1195 0.62 +2.0 1,224,036 - Synpcc7942_1195 0.63 +2.0 1,224,036 - Synpcc7942_1195 0.63 +1.7 1,224,076 - Synpcc7942_1195 0.71 -0.2 1,224,086 - Synpcc7942_1195 0.73 +1.0 1,224,111 - Synpcc7942_1195 0.78 -3.1 1,224,111 - Synpcc7942_1195 0.78 -0.4 1,224,145 + Synpcc7942_1195 0.85 +1.0 1,224,167 + Synpcc7942_1195 0.89 +2.2 1,224,279 + -0.5 1,224,279 + +0.3 1,224,279 + -1.6 1,224,280 - -0.0 1,224,287 - +1.1 1,224,287 - -0.7 1,224,292 - +2.6 1,224,292 - +1.5 1,224,432 + sipA Synpcc7942_1196 0.57 -0.5 1,224,443 + sipA Synpcc7942_1196 0.61 -1.5 1,224,454 - sipA Synpcc7942_1196 0.66 -0.9 1,224,475 - sipA Synpcc7942_1196 0.75 -1.3 1,224,475 - sipA Synpcc7942_1196 0.75 -0.9 1,224,495 + sipA Synpcc7942_1196 0.83 -2.4 1,224,529 + +2.6 1,224,529 + -1.7 1,224,530 + +1.1 1,224,537 - +4.1 1,224,537 - +1.1 1,224,559 - -1.4 1,224,561 + -0.4 1,224,587 - +0.0 1,224,593 - +2.1 1,224,622 - +0.1 1,224,623 - -0.7 1,224,630 - +2.1 1,224,663 - +2.1 1,224,683 - +1.1 1,224,684 - -1.4 1,224,712 + -0.7 1,224,716 + -0.2 1,224,721 + -0.5 1,224,753 - -0.1 1,224,753 - +1.1 1,224,754 - +1.1 1,224,772 + -0.8 1,224,772 + +2.6 1,224,772 + -0.1 1,224,792 + +0.4 1,224,839 + -0.2 1,224,839 + +0.1 1,224,842 - +1.6 1,224,846 - -0.3 1,224,846 - -0.9 1,224,847 - -0.1 1,224,847 - +2.1 1,224,853 - -0.6 1,224,871 + +0.8
Or see this region's nucleotide sequence