Experiment: H2 McCas with 1 hour oxygen exposure
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00335 and MMP_RS00340 are separated by 229 nucleotides MMP_RS00340 and MMP_RS00345 are separated by 64 nucleotides MMP_RS00345 and MMP_RS00350 are separated by 108 nucleotides MMP_RS00350 and MMP_RS00355 are separated by 18 nucleotides
MMP_RS00335: MMP_RS00335 - 5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase CofH, at 71,296 to 72,372
_RS00335
MMP_RS00340: MMP_RS00340 - 5 10-methylenetetrahydromethanopterin reductase, at 72,602 to 73,564
_RS00340
MMP_RS00345: MMP_RS00345 - DUF2666 domain-containing protein, at 73,629 to 74,051
_RS00345
MMP_RS00350: MMP_RS00350 - 50S ribosomal protein L18a, at 74,160 to 74,390
_RS00350
MMP_RS00355: MMP_RS00355 - translation initiation factor IF-6, at 74,409 to 75,092
_RS00355
Position (kb) 72
73
74 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 71.833 kb on - strand, within MMP_RS00335 at 72.007 kb on - strand, within MMP_RS00335 at 72.183 kb on - strand, within MMP_RS00335 at 72.256 kb on + strand, within MMP_RS00335 at 72.503 kb on - strand at 72.541 kb on + strand at 72.541 kb on + strand at 73.405 kb on + strand at 73.533 kb on - strand at 73.617 kb on + strand at 73.690 kb on + strand, within MMP_RS00345 at 73.821 kb on + strand, within MMP_RS00345 at 73.832 kb on - strand, within MMP_RS00345 at 73.857 kb on - strand, within MMP_RS00345 at 73.917 kb on - strand, within MMP_RS00345 at 74.078 kb on - strand at 74.080 kb on - strand at 74.109 kb on + strand at 74.110 kb on - strand at 74.111 kb on + strand at 74.113 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 1 hour oxygen exposure remove 71,833 - MMP_RS00335 0.50 -0.3 72,007 - MMP_RS00335 0.66 +0.3 72,183 - MMP_RS00335 0.82 +0.2 72,256 + MMP_RS00335 0.89 +1.1 72,503 - -0.4 72,541 + -2.8 72,541 + -1.9 73,405 + -1.4 73,533 - -3.1 73,617 + -0.2 73,690 + MMP_RS00345 0.14 +0.4 73,821 + MMP_RS00345 0.45 +1.2 73,832 - MMP_RS00345 0.48 +1.5 73,857 - MMP_RS00345 0.54 +1.2 73,917 - MMP_RS00345 0.68 +0.3 74,078 - +0.4 74,080 - +0.8 74,109 + +1.3 74,110 - +0.8 74,111 + +0.5 74,113 + +0.3
Or see this region's nucleotide sequence