Experiment: Bu1_100
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00581 and H281DRAFT_00582 are separated by 430 nucleotides H281DRAFT_00582 and H281DRAFT_00583 are separated by 188 nucleotides H281DRAFT_00583 and H281DRAFT_00584 overlap by 4 nucleotides
H281DRAFT_00581: H281DRAFT_00581 - Superfamily II DNA and RNA helicase, at 647,901 to 649,523
_00581
H281DRAFT_00582: H281DRAFT_00582 - Acyl-CoA-binding protein, at 649,954 to 650,223
_00582
H281DRAFT_00583: H281DRAFT_00583 - tRNA threonylcarbamoyladenosine biosynthesis protein TsaB, at 650,412 to 651,215
_00583
H281DRAFT_00584: H281DRAFT_00584 - [SSU ribosomal protein S18P]-alanine acetyltransferase, at 651,212 to 651,706
_00584
Position (kb) 649
650
651 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 649.134 kb on - strand at 649.549 kb on - strand at 649.642 kb on + strand at 649.846 kb on + strand at 649.847 kb on - strand at 649.847 kb on - strand at 649.848 kb on + strand at 649.849 kb on - strand at 649.852 kb on + strand at 649.852 kb on + strand at 649.853 kb on - strand at 649.876 kb on - strand at 649.911 kb on - strand at 649.997 kb on + strand, within H281DRAFT_00582 at 649.997 kb on + strand, within H281DRAFT_00582 at 649.997 kb on + strand, within H281DRAFT_00582 at 649.998 kb on - strand, within H281DRAFT_00582 at 649.998 kb on - strand, within H281DRAFT_00582 at 649.998 kb on - strand, within H281DRAFT_00582 at 650.051 kb on + strand, within H281DRAFT_00582 at 650.052 kb on - strand, within H281DRAFT_00582 at 650.052 kb on - strand, within H281DRAFT_00582 at 650.057 kb on + strand, within H281DRAFT_00582 at 650.057 kb on + strand, within H281DRAFT_00582 at 650.057 kb on + strand, within H281DRAFT_00582 at 650.058 kb on - strand, within H281DRAFT_00582 at 650.058 kb on - strand, within H281DRAFT_00582 at 650.058 kb on - strand, within H281DRAFT_00582 at 650.123 kb on + strand, within H281DRAFT_00582 at 650.123 kb on + strand, within H281DRAFT_00582 at 650.123 kb on + strand, within H281DRAFT_00582 at 650.124 kb on - strand, within H281DRAFT_00582 at 650.124 kb on - strand, within H281DRAFT_00582 at 650.124 kb on - strand, within H281DRAFT_00582 at 650.133 kb on - strand, within H281DRAFT_00582 at 650.248 kb on + strand at 650.248 kb on + strand at 650.265 kb on + strand at 650.265 kb on + strand at 650.265 kb on + strand at 650.266 kb on - strand at 650.298 kb on + strand at 650.299 kb on - strand at 650.299 kb on - strand at 650.399 kb on + strand at 650.399 kb on + strand at 650.400 kb on - strand at 650.400 kb on - strand at 650.400 kb on - strand at 650.404 kb on + strand at 650.404 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bu1_100 remove 649,134 - +2.1 649,549 - +0.1 649,642 + -0.4 649,846 + -0.1 649,847 - +0.5 649,847 - +0.7 649,848 + -0.7 649,849 - -1.4 649,852 + +2.0 649,852 + +0.3 649,853 - +1.1 649,876 - +0.3 649,911 - -0.8 649,997 + H281DRAFT_00582 0.16 +0.1 649,997 + H281DRAFT_00582 0.16 +0.1 649,997 + H281DRAFT_00582 0.16 -0.6 649,998 - H281DRAFT_00582 0.16 -2.4 649,998 - H281DRAFT_00582 0.16 -1.2 649,998 - H281DRAFT_00582 0.16 -2.3 650,051 + H281DRAFT_00582 0.36 -0.3 650,052 - H281DRAFT_00582 0.36 +0.2 650,052 - H281DRAFT_00582 0.36 +1.1 650,057 + H281DRAFT_00582 0.38 +0.6 650,057 + H281DRAFT_00582 0.38 -0.3 650,057 + H281DRAFT_00582 0.38 +1.9 650,058 - H281DRAFT_00582 0.39 +0.1 650,058 - H281DRAFT_00582 0.39 -0.0 650,058 - H281DRAFT_00582 0.39 +0.7 650,123 + H281DRAFT_00582 0.63 -0.6 650,123 + H281DRAFT_00582 0.63 -1.6 650,123 + H281DRAFT_00582 0.63 -1.1 650,124 - H281DRAFT_00582 0.63 -0.4 650,124 - H281DRAFT_00582 0.63 -1.1 650,124 - H281DRAFT_00582 0.63 +0.2 650,133 - H281DRAFT_00582 0.66 +0.5 650,248 + -0.4 650,248 + +1.0 650,265 + +2.4 650,265 + +0.2 650,265 + +1.3 650,266 - +0.2 650,298 + -0.7 650,299 - +0.3 650,299 - -1.0 650,399 + +1.1 650,399 + +0.4 650,400 - +1.1 650,400 - +0.1 650,400 - -2.8 650,404 + +0.6 650,404 + -1.0
Or see this region's nucleotide sequence