Experiment: BHIS with Dimethyl Sulfoxide 0.2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1993 and BT1994 overlap by 32 nucleotides BT1994 and BT1995 are separated by 69 nucleotides BT1995 and BT1996 are separated by 172 nucleotides
BT1993: BT1993 - putative cell wall-associated protein precursor (NCBI ptt file), at 2,504,367 to 2,508,317
BT1993
BT1994: BT1994 - hypothetical protein (NCBI ptt file), at 2,508,286 to 2,508,606
BT1994
BT1995: BT1995 - hypothetical protein (NCBI ptt file), at 2,508,676 to 2,509,260
BT1995
BT1996: BT1996 - transposase (NCBI ptt file), at 2,509,433 to 2,510,650
BT1996
Position (kb)
2508
2509 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2507.326 kb on + strand, within BT1993 at 2507.327 kb on - strand, within BT1993 at 2507.343 kb on + strand, within BT1993 at 2507.344 kb on - strand, within BT1993 at 2507.354 kb on - strand, within BT1993 at 2507.374 kb on - strand, within BT1993 at 2507.498 kb on + strand, within BT1993 at 2507.498 kb on + strand, within BT1993 at 2507.503 kb on - strand, within BT1993 at 2507.505 kb on - strand, within BT1993 at 2507.545 kb on - strand, within BT1993 at 2507.591 kb on + strand, within BT1993 at 2507.622 kb on - strand, within BT1993 at 2507.634 kb on + strand, within BT1993 at 2507.657 kb on + strand, within BT1993 at 2507.658 kb on - strand, within BT1993 at 2507.658 kb on - strand, within BT1993 at 2507.669 kb on - strand, within BT1993 at 2507.741 kb on - strand, within BT1993 at 2507.748 kb on - strand, within BT1993 at 2507.783 kb on - strand, within BT1993 at 2507.783 kb on - strand, within BT1993 at 2507.818 kb on + strand, within BT1993 at 2507.820 kb on + strand, within BT1993 at 2507.821 kb on - strand, within BT1993 at 2507.869 kb on + strand, within BT1993 at 2507.870 kb on - strand, within BT1993 at 2507.985 kb on + strand at 2508.068 kb on + strand at 2508.068 kb on + strand at 2508.128 kb on + strand at 2508.154 kb on - strand at 2508.156 kb on - strand at 2508.156 kb on - strand at 2508.191 kb on + strand at 2508.315 kb on + strand at 2508.361 kb on + strand, within BT1994 at 2508.367 kb on - strand at 2508.374 kb on - strand, within BT1994 at 2508.377 kb on + strand, within BT1994 at 2508.500 kb on + strand, within BT1994 at 2508.513 kb on + strand, within BT1994 at 2508.525 kb on + strand, within BT1994 at 2508.526 kb on - strand, within BT1994 at 2508.566 kb on + strand, within BT1994 at 2508.594 kb on + strand at 2508.604 kb on + strand at 2508.605 kb on - strand at 2508.617 kb on + strand at 2508.618 kb on - strand at 2508.619 kb on + strand at 2508.785 kb on - strand, within BT1995 at 2508.857 kb on + strand, within BT1995 at 2508.858 kb on - strand, within BT1995 at 2508.893 kb on - strand, within BT1995 at 2508.934 kb on + strand, within BT1995 at 2508.934 kb on + strand, within BT1995 at 2508.948 kb on - strand, within BT1995 at 2508.948 kb on - strand, within BT1995 at 2509.017 kb on + strand, within BT1995 at 2509.021 kb on + strand, within BT1995 at 2509.022 kb on - strand, within BT1995 at 2509.033 kb on + strand, within BT1995 at 2509.080 kb on + strand, within BT1995 at 2509.080 kb on + strand, within BT1995 at 2509.081 kb on - strand, within BT1995 at 2509.085 kb on + strand, within BT1995 at 2509.085 kb on + strand, within BT1995 at 2509.141 kb on + strand, within BT1995 at 2509.176 kb on - strand, within BT1995 at 2509.176 kb on - strand, within BT1995 at 2509.207 kb on + strand at 2509.236 kb on + strand at 2509.239 kb on + strand at 2509.240 kb on - strand at 2509.282 kb on + strand at 2509.282 kb on + strand at 2509.282 kb on + strand at 2509.286 kb on + strand at 2509.335 kb on - strand at 2509.433 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.2 vol% remove 2,507,326 + BT1993 0.75 -0.8 2,507,327 - BT1993 0.75 +0.2 2,507,343 + BT1993 0.75 -0.1 2,507,344 - BT1993 0.75 -1.0 2,507,354 - BT1993 0.76 -0.8 2,507,374 - BT1993 0.76 -1.3 2,507,498 + BT1993 0.79 +0.2 2,507,498 + BT1993 0.79 -1.1 2,507,503 - BT1993 0.79 -0.8 2,507,505 - BT1993 0.79 +0.2 2,507,545 - BT1993 0.80 -0.8 2,507,591 + BT1993 0.82 +0.2 2,507,622 - BT1993 0.82 -0.4 2,507,634 + BT1993 0.83 -0.8 2,507,657 + BT1993 0.83 -0.8 2,507,658 - BT1993 0.83 -0.8 2,507,658 - BT1993 0.83 -1.5 2,507,669 - BT1993 0.84 -1.6 2,507,741 - BT1993 0.85 +0.7 2,507,748 - BT1993 0.86 -0.9 2,507,783 - BT1993 0.86 -0.8 2,507,783 - BT1993 0.86 -1.4 2,507,818 + BT1993 0.87 -0.4 2,507,820 + BT1993 0.87 +0.2 2,507,821 - BT1993 0.87 -0.2 2,507,869 + BT1993 0.89 +0.4 2,507,870 - BT1993 0.89 +0.7 2,507,985 + -0.8 2,508,068 + +1.2 2,508,068 + -0.5 2,508,128 + -0.8 2,508,154 - -0.4 2,508,156 - -0.8 2,508,156 - -1.5 2,508,191 + -1.4 2,508,315 + -1.2 2,508,361 + BT1994 0.23 +1.2 2,508,367 - -2.2 2,508,374 - BT1994 0.27 -1.7 2,508,377 + BT1994 0.28 +0.7 2,508,500 + BT1994 0.67 -1.4 2,508,513 + BT1994 0.71 -3.2 2,508,525 + BT1994 0.74 -3.0 2,508,526 - BT1994 0.75 +0.2 2,508,566 + BT1994 0.87 -2.6 2,508,594 + -0.6 2,508,604 + -0.4 2,508,605 - +0.2 2,508,617 + +0.2 2,508,618 - -2.8 2,508,619 + +0.2 2,508,785 - BT1995 0.19 -2.4 2,508,857 + BT1995 0.31 +0.2 2,508,858 - BT1995 0.31 +0.2 2,508,893 - BT1995 0.37 +0.2 2,508,934 + BT1995 0.44 +1.2 2,508,934 + BT1995 0.44 -1.0 2,508,948 - BT1995 0.46 -1.4 2,508,948 - BT1995 0.46 -0.7 2,509,017 + BT1995 0.58 +0.2 2,509,021 + BT1995 0.59 -1.3 2,509,022 - BT1995 0.59 -0.3 2,509,033 + BT1995 0.61 -1.6 2,509,080 + BT1995 0.69 -1.0 2,509,080 + BT1995 0.69 -1.2 2,509,081 - BT1995 0.69 +0.6 2,509,085 + BT1995 0.70 +0.2 2,509,085 + BT1995 0.70 -0.9 2,509,141 + BT1995 0.79 -1.5 2,509,176 - BT1995 0.85 +0.2 2,509,176 - BT1995 0.85 -1.4 2,509,207 + -3.7 2,509,236 + +1.2 2,509,239 + -1.7 2,509,240 - -1.2 2,509,282 + +0.4 2,509,282 + +0.2 2,509,282 + -1.1 2,509,286 + -1.8 2,509,335 - -1.0 2,509,433 - -0.6
Or see this region's nucleotide sequence