Experiment: BHIS with Dimethyl Sulfoxide 0.2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1501 and BT1502 are separated by 77 nucleotides BT1502 and BT1503 are separated by 492 nucleotides
BT1501: BT1501 - major outer membrane protein OmpA (NCBI ptt file), at 1,850,513 to 1,851,604
BT1501
BT1502: BT1502 - hypothetical protein (NCBI ptt file), at 1,851,682 to 1,854,519
BT1502
BT1503: BT1503 - integrase (NCBI ptt file), at 1,855,012 to 1,856,022
BT1503
Position (kb)
1851
1852
1853
1854
1855 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1851.150 kb on + strand, within BT1501 at 1851.150 kb on + strand, within BT1501 at 1851.303 kb on + strand, within BT1501 at 1851.307 kb on + strand, within BT1501 at 1851.430 kb on + strand, within BT1501 at 1851.473 kb on + strand, within BT1501 at 1851.557 kb on + strand at 1851.558 kb on - strand at 1851.573 kb on - strand at 1851.574 kb on + strand at 1851.595 kb on + strand at 1851.604 kb on + strand at 1851.612 kb on + strand at 1851.643 kb on + strand at 1851.662 kb on - strand at 1851.663 kb on + strand at 1851.719 kb on + strand at 1851.733 kb on - strand at 1851.753 kb on + strand at 1851.801 kb on + strand at 1851.802 kb on - strand at 1851.807 kb on + strand at 1851.807 kb on + strand at 1851.808 kb on - strand at 1851.846 kb on + strand at 1851.858 kb on + strand at 1851.946 kb on - strand at 1851.948 kb on + strand at 1851.949 kb on - strand at 1851.949 kb on - strand at 1851.954 kb on + strand at 1851.955 kb on - strand at 1851.963 kb on + strand at 1852.003 kb on + strand, within BT1502 at 1852.004 kb on - strand, within BT1502 at 1852.059 kb on + strand, within BT1502 at 1852.060 kb on - strand, within BT1502 at 1852.115 kb on - strand, within BT1502 at 1852.162 kb on - strand, within BT1502 at 1852.171 kb on + strand, within BT1502 at 1852.171 kb on + strand, within BT1502 at 1852.214 kb on + strand, within BT1502 at 1852.294 kb on + strand, within BT1502 at 1852.344 kb on + strand, within BT1502 at 1852.379 kb on - strand, within BT1502 at 1852.383 kb on + strand, within BT1502 at 1852.383 kb on + strand, within BT1502 at 1852.384 kb on - strand, within BT1502 at 1852.420 kb on + strand, within BT1502 at 1852.442 kb on + strand, within BT1502 at 1852.443 kb on - strand, within BT1502 at 1852.455 kb on + strand, within BT1502 at 1852.456 kb on - strand, within BT1502 at 1852.470 kb on + strand, within BT1502 at 1852.633 kb on - strand, within BT1502 at 1852.771 kb on - strand, within BT1502 at 1852.842 kb on + strand, within BT1502 at 1852.845 kb on - strand, within BT1502 at 1852.866 kb on - strand, within BT1502 at 1852.866 kb on - strand, within BT1502 at 1852.866 kb on - strand, within BT1502 at 1852.869 kb on + strand, within BT1502 at 1852.885 kb on - strand, within BT1502 at 1852.886 kb on + strand, within BT1502 at 1852.887 kb on - strand, within BT1502 at 1852.906 kb on - strand, within BT1502 at 1853.010 kb on + strand, within BT1502 at 1853.055 kb on + strand, within BT1502 at 1853.056 kb on - strand, within BT1502 at 1853.089 kb on + strand, within BT1502 at 1853.107 kb on + strand, within BT1502 at 1853.108 kb on - strand, within BT1502 at 1853.124 kb on + strand, within BT1502 at 1853.125 kb on - strand, within BT1502 at 1853.125 kb on - strand, within BT1502 at 1853.202 kb on + strand, within BT1502 at 1853.211 kb on + strand at 1853.286 kb on + strand, within BT1502 at 1853.306 kb on + strand, within BT1502 at 1853.306 kb on + strand, within BT1502 at 1853.306 kb on + strand, within BT1502 at 1853.306 kb on + strand, within BT1502 at 1853.354 kb on + strand, within BT1502 at 1853.385 kb on + strand, within BT1502 at 1853.385 kb on + strand, within BT1502 at 1853.417 kb on + strand, within BT1502 at 1853.480 kb on + strand, within BT1502 at 1853.493 kb on - strand, within BT1502 at 1853.496 kb on + strand, within BT1502 at 1853.503 kb on - strand, within BT1502 at 1853.514 kb on + strand, within BT1502 at 1853.524 kb on - strand, within BT1502 at 1853.540 kb on - strand, within BT1502 at 1853.595 kb on + strand, within BT1502 at 1853.599 kb on - strand, within BT1502 at 1853.685 kb on + strand, within BT1502 at 1853.685 kb on + strand, within BT1502 at 1853.687 kb on + strand, within BT1502 at 1853.687 kb on + strand, within BT1502 at 1853.687 kb on + strand, within BT1502 at 1853.757 kb on - strand, within BT1502 at 1853.759 kb on + strand, within BT1502 at 1853.759 kb on + strand, within BT1502 at 1853.760 kb on - strand, within BT1502 at 1853.968 kb on - strand, within BT1502 at 1853.968 kb on - strand, within BT1502 at 1854.005 kb on + strand, within BT1502 at 1854.005 kb on + strand, within BT1502 at 1854.010 kb on + strand, within BT1502 at 1854.055 kb on - strand, within BT1502 at 1854.055 kb on - strand, within BT1502 at 1854.055 kb on - strand, within BT1502 at 1854.058 kb on + strand, within BT1502 at 1854.058 kb on + strand, within BT1502 at 1854.172 kb on - strand, within BT1502 at 1854.184 kb on - strand, within BT1502 at 1854.205 kb on - strand at 1854.205 kb on - strand, within BT1502 at 1854.210 kb on + strand, within BT1502 at 1854.210 kb on + strand, within BT1502 at 1854.217 kb on - strand, within BT1502 at 1854.252 kb on + strand at 1854.445 kb on - strand at 1854.445 kb on - strand at 1854.454 kb on - strand at 1854.454 kb on - strand at 1854.714 kb on + strand at 1854.768 kb on - strand at 1854.775 kb on - strand at 1854.792 kb on - strand at 1854.838 kb on + strand at 1854.842 kb on + strand at 1854.914 kb on - strand at 1854.914 kb on - strand at 1855.138 kb on - strand, within BT1503 at 1855.175 kb on + strand, within BT1503 at 1855.175 kb on + strand, within BT1503 at 1855.195 kb on - strand, within BT1503 at 1855.209 kb on - strand, within BT1503 at 1855.344 kb on - strand, within BT1503 at 1855.356 kb on + strand, within BT1503 at 1855.382 kb on - strand, within BT1503 at 1855.384 kb on - strand, within BT1503 at 1855.384 kb on - strand, within BT1503
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.2 vol% remove 1,851,150 + BT1501 0.58 -2.1 1,851,150 + BT1501 0.58 +1.3 1,851,303 + BT1501 0.72 -2.9 1,851,307 + BT1501 0.73 -2.1 1,851,430 + BT1501 0.84 -1.3 1,851,473 + BT1501 0.88 -2.5 1,851,557 + +1.3 1,851,558 - -1.5 1,851,573 - -1.0 1,851,574 + -1.7 1,851,595 + -0.3 1,851,604 + +1.3 1,851,612 + -0.5 1,851,643 + +0.3 1,851,662 - -1.3 1,851,663 + +0.3 1,851,719 + -1.7 1,851,733 - -1.0 1,851,753 + -0.5 1,851,801 + -0.1 1,851,802 - +2.6 1,851,807 + -2.3 1,851,807 + -1.1 1,851,808 - +0.3 1,851,846 + +1.3 1,851,858 + -3.2 1,851,946 - +0.9 1,851,948 + -1.6 1,851,949 - -1.3 1,851,949 - +0.3 1,851,954 + +0.1 1,851,955 - -2.7 1,851,963 + -0.7 1,852,003 + BT1502 0.11 -2.3 1,852,004 - BT1502 0.11 -1.3 1,852,059 + BT1502 0.13 +0.9 1,852,060 - BT1502 0.13 +1.3 1,852,115 - BT1502 0.15 -0.7 1,852,162 - BT1502 0.17 +0.3 1,852,171 + BT1502 0.17 -1.7 1,852,171 + BT1502 0.17 +0.3 1,852,214 + BT1502 0.19 -1.1 1,852,294 + BT1502 0.22 -2.1 1,852,344 + BT1502 0.23 +1.9 1,852,379 - BT1502 0.25 +1.0 1,852,383 + BT1502 0.25 -1.1 1,852,383 + BT1502 0.25 -0.3 1,852,384 - BT1502 0.25 +0.1 1,852,420 + BT1502 0.26 +0.3 1,852,442 + BT1502 0.27 -0.2 1,852,443 - BT1502 0.27 -0.3 1,852,455 + BT1502 0.27 -0.9 1,852,456 - BT1502 0.27 -1.7 1,852,470 + BT1502 0.28 -0.3 1,852,633 - BT1502 0.34 +0.3 1,852,771 - BT1502 0.38 +0.3 1,852,842 + BT1502 0.41 +1.3 1,852,845 - BT1502 0.41 -1.7 1,852,866 - BT1502 0.42 -0.7 1,852,866 - BT1502 0.42 +0.3 1,852,866 - BT1502 0.42 -2.7 1,852,869 + BT1502 0.42 +0.1 1,852,885 - BT1502 0.42 -0.7 1,852,886 + BT1502 0.42 -1.3 1,852,887 - BT1502 0.42 +0.3 1,852,906 - BT1502 0.43 +0.9 1,853,010 + BT1502 0.47 -1.3 1,853,055 + BT1502 0.48 -0.3 1,853,056 - BT1502 0.48 +0.9 1,853,089 + BT1502 0.50 -1.3 1,853,107 + BT1502 0.50 -0.7 1,853,108 - BT1502 0.50 -1.7 1,853,124 + BT1502 0.51 -1.3 1,853,125 - BT1502 0.51 -0.7 1,853,125 - BT1502 0.51 -0.7 1,853,202 + BT1502 0.54 -1.2 1,853,211 + +0.3 1,853,286 + BT1502 0.57 -0.1 1,853,306 + BT1502 0.57 -0.7 1,853,306 + BT1502 0.57 +0.9 1,853,306 + BT1502 0.57 -0.7 1,853,306 + BT1502 0.57 -2.7 1,853,354 + BT1502 0.59 -0.4 1,853,385 + BT1502 0.60 -0.7 1,853,385 + BT1502 0.60 +0.3 1,853,417 + BT1502 0.61 +0.9 1,853,480 + BT1502 0.63 -1.6 1,853,493 - BT1502 0.64 +1.1 1,853,496 + BT1502 0.64 +0.3 1,853,503 - BT1502 0.64 +0.9 1,853,514 + BT1502 0.65 -0.5 1,853,524 - BT1502 0.65 -0.7 1,853,540 - BT1502 0.65 +0.3 1,853,595 + BT1502 0.67 +0.3 1,853,599 - BT1502 0.68 -0.7 1,853,685 + BT1502 0.71 -2.5 1,853,685 + BT1502 0.71 -0.5 1,853,687 + BT1502 0.71 +0.2 1,853,687 + BT1502 0.71 -0.1 1,853,687 + BT1502 0.71 +0.3 1,853,757 - BT1502 0.73 -0.7 1,853,759 + BT1502 0.73 +0.3 1,853,759 + BT1502 0.73 -0.8 1,853,760 - BT1502 0.73 -2.3 1,853,968 - BT1502 0.81 -2.7 1,853,968 - BT1502 0.81 -0.5 1,854,005 + BT1502 0.82 -1.3 1,854,005 + BT1502 0.82 -1.3 1,854,010 + BT1502 0.82 -0.5 1,854,055 - BT1502 0.84 -2.3 1,854,055 - BT1502 0.84 +0.0 1,854,055 - BT1502 0.84 -0.5 1,854,058 + BT1502 0.84 -0.7 1,854,058 + BT1502 0.84 -0.3 1,854,172 - BT1502 0.88 -4.3 1,854,184 - BT1502 0.88 -2.1 1,854,205 - -1.3 1,854,205 - BT1502 0.89 +0.9 1,854,210 + BT1502 0.89 -1.0 1,854,210 + BT1502 0.89 +0.7 1,854,217 - BT1502 0.89 -2.1 1,854,252 + -0.6 1,854,445 - +1.3 1,854,445 - -0.1 1,854,454 - -1.3 1,854,454 - -1.7 1,854,714 + -1.3 1,854,768 - -2.3 1,854,775 - -1.5 1,854,792 - -0.1 1,854,838 + -2.0 1,854,842 + +0.9 1,854,914 - +0.3 1,854,914 - -1.5 1,855,138 - BT1503 0.12 -1.3 1,855,175 + BT1503 0.16 -1.1 1,855,175 + BT1503 0.16 -0.8 1,855,195 - BT1503 0.18 -0.7 1,855,209 - BT1503 0.19 -0.6 1,855,344 - BT1503 0.33 +0.3 1,855,356 + BT1503 0.34 -0.4 1,855,382 - BT1503 0.37 +0.3 1,855,384 - BT1503 0.37 -0.7 1,855,384 - BT1503 0.37 -0.7
Or see this region's nucleotide sequence