Experiment: BHIS with Dimethyl Sulfoxide 0.2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1500 and BT1501 are separated by 327 nucleotides BT1501 and BT1502 are separated by 77 nucleotides
BT1500: BT1500 - Ribonuclease G (NCBI ptt file), at 1,848,611 to 1,850,185
BT1500
BT1501: BT1501 - major outer membrane protein OmpA (NCBI ptt file), at 1,850,513 to 1,851,604
BT1501
BT1502: BT1502 - hypothetical protein (NCBI ptt file), at 1,851,682 to 1,854,519
BT1502
Position (kb)
1850
1851
1852 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1850.159 kb on - strand at 1850.179 kb on + strand at 1850.227 kb on - strand at 1850.274 kb on + strand at 1850.344 kb on - strand at 1850.344 kb on - strand at 1850.360 kb on - strand at 1850.360 kb on - strand at 1850.362 kb on + strand at 1850.363 kb on - strand at 1850.425 kb on + strand at 1850.428 kb on - strand at 1850.428 kb on - strand at 1850.435 kb on - strand at 1850.494 kb on + strand at 1850.524 kb on + strand at 1850.530 kb on + strand at 1850.557 kb on + strand at 1850.557 kb on + strand at 1850.558 kb on - strand at 1850.558 kb on - strand at 1850.559 kb on + strand at 1850.559 kb on + strand at 1850.583 kb on + strand at 1850.612 kb on - strand at 1851.150 kb on + strand, within BT1501 at 1851.150 kb on + strand, within BT1501 at 1851.303 kb on + strand, within BT1501 at 1851.307 kb on + strand, within BT1501 at 1851.430 kb on + strand, within BT1501 at 1851.473 kb on + strand, within BT1501 at 1851.557 kb on + strand at 1851.558 kb on - strand at 1851.573 kb on - strand at 1851.574 kb on + strand at 1851.595 kb on + strand at 1851.604 kb on + strand at 1851.612 kb on + strand at 1851.643 kb on + strand at 1851.662 kb on - strand at 1851.663 kb on + strand at 1851.719 kb on + strand at 1851.733 kb on - strand at 1851.753 kb on + strand at 1851.801 kb on + strand at 1851.802 kb on - strand at 1851.807 kb on + strand at 1851.807 kb on + strand at 1851.808 kb on - strand at 1851.846 kb on + strand at 1851.858 kb on + strand at 1851.946 kb on - strand at 1851.948 kb on + strand at 1851.949 kb on - strand at 1851.949 kb on - strand at 1851.954 kb on + strand at 1851.955 kb on - strand at 1851.963 kb on + strand at 1852.003 kb on + strand, within BT1502 at 1852.004 kb on - strand, within BT1502 at 1852.059 kb on + strand, within BT1502 at 1852.060 kb on - strand, within BT1502 at 1852.115 kb on - strand, within BT1502 at 1852.162 kb on - strand, within BT1502 at 1852.171 kb on + strand, within BT1502 at 1852.171 kb on + strand, within BT1502 at 1852.214 kb on + strand, within BT1502 at 1852.294 kb on + strand, within BT1502 at 1852.344 kb on + strand, within BT1502 at 1852.379 kb on - strand, within BT1502 at 1852.383 kb on + strand, within BT1502 at 1852.383 kb on + strand, within BT1502 at 1852.384 kb on - strand, within BT1502 at 1852.420 kb on + strand, within BT1502 at 1852.442 kb on + strand, within BT1502 at 1852.443 kb on - strand, within BT1502 at 1852.455 kb on + strand, within BT1502 at 1852.456 kb on - strand, within BT1502 at 1852.470 kb on + strand, within BT1502
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.2 vol% remove 1,850,159 - +0.6 1,850,179 + -0.1 1,850,227 - -3.3 1,850,274 + -1.7 1,850,344 - -1.7 1,850,344 - +0.4 1,850,360 - +0.3 1,850,360 - +0.3 1,850,362 + -0.3 1,850,363 - -0.1 1,850,425 + -0.4 1,850,428 - -1.2 1,850,428 - -2.3 1,850,435 - -0.1 1,850,494 + -1.3 1,850,524 + -0.6 1,850,530 + +2.3 1,850,557 + -2.3 1,850,557 + +0.3 1,850,558 - -2.1 1,850,558 - -0.7 1,850,559 + -0.7 1,850,559 + -0.5 1,850,583 + -0.1 1,850,612 - -2.3 1,851,150 + BT1501 0.58 -2.1 1,851,150 + BT1501 0.58 +1.3 1,851,303 + BT1501 0.72 -2.9 1,851,307 + BT1501 0.73 -2.1 1,851,430 + BT1501 0.84 -1.3 1,851,473 + BT1501 0.88 -2.5 1,851,557 + +1.3 1,851,558 - -1.5 1,851,573 - -1.0 1,851,574 + -1.7 1,851,595 + -0.3 1,851,604 + +1.3 1,851,612 + -0.5 1,851,643 + +0.3 1,851,662 - -1.3 1,851,663 + +0.3 1,851,719 + -1.7 1,851,733 - -1.0 1,851,753 + -0.5 1,851,801 + -0.1 1,851,802 - +2.6 1,851,807 + -2.3 1,851,807 + -1.1 1,851,808 - +0.3 1,851,846 + +1.3 1,851,858 + -3.2 1,851,946 - +0.9 1,851,948 + -1.6 1,851,949 - -1.3 1,851,949 - +0.3 1,851,954 + +0.1 1,851,955 - -2.7 1,851,963 + -0.7 1,852,003 + BT1502 0.11 -2.3 1,852,004 - BT1502 0.11 -1.3 1,852,059 + BT1502 0.13 +0.9 1,852,060 - BT1502 0.13 +1.3 1,852,115 - BT1502 0.15 -0.7 1,852,162 - BT1502 0.17 +0.3 1,852,171 + BT1502 0.17 -1.7 1,852,171 + BT1502 0.17 +0.3 1,852,214 + BT1502 0.19 -1.1 1,852,294 + BT1502 0.22 -2.1 1,852,344 + BT1502 0.23 +1.9 1,852,379 - BT1502 0.25 +1.0 1,852,383 + BT1502 0.25 -1.1 1,852,383 + BT1502 0.25 -0.3 1,852,384 - BT1502 0.25 +0.1 1,852,420 + BT1502 0.26 +0.3 1,852,442 + BT1502 0.27 -0.2 1,852,443 - BT1502 0.27 -0.3 1,852,455 + BT1502 0.27 -0.9 1,852,456 - BT1502 0.27 -1.7 1,852,470 + BT1502 0.28 -0.3
Or see this region's nucleotide sequence