Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2403 and Synpcc7942_2404 are separated by 58 nucleotides Synpcc7942_2404 and tRNA-Phe overlap by 35 nucleotides tRNA-Phe and Synpcc7942_2405 are separated by 153 nucleotides Synpcc7942_2405 and thiO are separated by 57 nucleotides
Synpcc7942_2403: Synpcc7942_2403 - hypothetical protein, at 2,477,464 to 2,477,748
_2403
Synpcc7942_2404: Synpcc7942_2404 - hypothetical protein, at 2,477,807 to 2,478,547
_2404
Synpcc7942_R0014: tRNA-Phe - tRNA-Phe, at 2,478,513 to 2,478,585
tRNA-Phe
Synpcc7942_2405: Synpcc7942_2405 - hypothetical protein, at 2,478,739 to 2,478,993
_2405
Synpcc7942_2406: thiO - FAD dependent oxidoreductase, at 2,479,051 to 2,480,190
thiO
Position (kb)
2478
2479 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2477.742 kb on + strand at 2477.790 kb on + strand at 2477.791 kb on - strand at 2477.798 kb on - strand at 2477.801 kb on - strand at 2477.874 kb on - strand at 2477.880 kb on - strand at 2477.920 kb on + strand, within Synpcc7942_2404 at 2477.925 kb on + strand, within Synpcc7942_2404 at 2477.933 kb on - strand, within Synpcc7942_2404 at 2477.947 kb on + strand, within Synpcc7942_2404 at 2477.949 kb on + strand, within Synpcc7942_2404 at 2477.976 kb on - strand, within Synpcc7942_2404 at 2477.987 kb on + strand, within Synpcc7942_2404 at 2477.997 kb on + strand, within Synpcc7942_2404 at 2478.011 kb on - strand, within Synpcc7942_2404 at 2478.021 kb on - strand, within Synpcc7942_2404 at 2478.021 kb on - strand, within Synpcc7942_2404 at 2478.027 kb on - strand, within Synpcc7942_2404 at 2478.047 kb on + strand, within Synpcc7942_2404 at 2478.060 kb on + strand, within Synpcc7942_2404 at 2478.061 kb on - strand, within Synpcc7942_2404 at 2478.068 kb on - strand, within Synpcc7942_2404 at 2478.172 kb on - strand, within Synpcc7942_2404 at 2478.191 kb on + strand, within Synpcc7942_2404 at 2478.191 kb on + strand, within Synpcc7942_2404 at 2478.199 kb on - strand, within Synpcc7942_2404 at 2478.199 kb on - strand, within Synpcc7942_2404 at 2478.236 kb on - strand, within Synpcc7942_2404 at 2478.256 kb on + strand, within Synpcc7942_2404 at 2478.261 kb on + strand, within Synpcc7942_2404 at 2478.261 kb on + strand, within Synpcc7942_2404 at 2478.323 kb on + strand, within Synpcc7942_2404 at 2478.331 kb on - strand, within Synpcc7942_2404 at 2478.347 kb on + strand, within Synpcc7942_2404 at 2478.347 kb on + strand, within Synpcc7942_2404 at 2478.365 kb on + strand, within Synpcc7942_2404 at 2478.375 kb on + strand, within Synpcc7942_2404 at 2478.380 kb on + strand, within Synpcc7942_2404 at 2478.389 kb on + strand, within Synpcc7942_2404 at 2478.419 kb on + strand, within Synpcc7942_2404 at 2478.613 kb on + strand at 2478.626 kb on + strand at 2478.629 kb on + strand at 2478.631 kb on - strand at 2478.684 kb on - strand at 2478.687 kb on + strand at 2478.695 kb on - strand at 2478.703 kb on - strand at 2478.713 kb on + strand at 2478.730 kb on + strand at 2478.731 kb on + strand at 2478.731 kb on + strand at 2478.731 kb on + strand at 2478.736 kb on + strand at 2478.736 kb on + strand at 2478.736 kb on + strand at 2478.739 kb on - strand at 2478.739 kb on - strand at 2478.739 kb on - strand at 2478.744 kb on - strand at 2478.744 kb on - strand at 2478.744 kb on - strand at 2478.772 kb on + strand, within Synpcc7942_2405 at 2478.794 kb on + strand, within Synpcc7942_2405 at 2478.828 kb on + strand, within Synpcc7942_2405 at 2478.881 kb on - strand, within Synpcc7942_2405 at 2478.881 kb on - strand, within Synpcc7942_2405 at 2478.913 kb on + strand, within Synpcc7942_2405 at 2478.919 kb on + strand, within Synpcc7942_2405 at 2478.921 kb on - strand, within Synpcc7942_2405 at 2478.921 kb on - strand, within Synpcc7942_2405 at 2478.924 kb on + strand, within Synpcc7942_2405 at 2478.924 kb on + strand, within Synpcc7942_2405 at 2478.929 kb on - strand, within Synpcc7942_2405 at 2478.932 kb on - strand at 2478.995 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 2,477,742 + +1.0 2,477,790 + +0.1 2,477,791 - -1.6 2,477,798 - -1.0 2,477,801 - +1.3 2,477,874 - -0.7 2,477,880 - +1.0 2,477,920 + Synpcc7942_2404 0.15 +1.5 2,477,925 + Synpcc7942_2404 0.16 +0.1 2,477,933 - Synpcc7942_2404 0.17 -1.1 2,477,947 + Synpcc7942_2404 0.19 -0.0 2,477,949 + Synpcc7942_2404 0.19 +1.0 2,477,976 - Synpcc7942_2404 0.23 -0.7 2,477,987 + Synpcc7942_2404 0.24 -0.0 2,477,997 + Synpcc7942_2404 0.26 -1.3 2,478,011 - Synpcc7942_2404 0.28 -0.9 2,478,021 - Synpcc7942_2404 0.29 +0.2 2,478,021 - Synpcc7942_2404 0.29 -0.5 2,478,027 - Synpcc7942_2404 0.30 -1.4 2,478,047 + Synpcc7942_2404 0.32 -0.3 2,478,060 + Synpcc7942_2404 0.34 -3.5 2,478,061 - Synpcc7942_2404 0.34 -0.4 2,478,068 - Synpcc7942_2404 0.35 -0.8 2,478,172 - Synpcc7942_2404 0.49 -0.9 2,478,191 + Synpcc7942_2404 0.52 -1.1 2,478,191 + Synpcc7942_2404 0.52 -0.6 2,478,199 - Synpcc7942_2404 0.53 -1.2 2,478,199 - Synpcc7942_2404 0.53 -0.3 2,478,236 - Synpcc7942_2404 0.58 -0.2 2,478,256 + Synpcc7942_2404 0.61 -0.7 2,478,261 + Synpcc7942_2404 0.61 -1.6 2,478,261 + Synpcc7942_2404 0.61 +1.3 2,478,323 + Synpcc7942_2404 0.70 +1.0 2,478,331 - Synpcc7942_2404 0.71 +0.4 2,478,347 + Synpcc7942_2404 0.73 -1.1 2,478,347 + Synpcc7942_2404 0.73 -1.6 2,478,365 + Synpcc7942_2404 0.75 -0.9 2,478,375 + Synpcc7942_2404 0.77 -0.6 2,478,380 + Synpcc7942_2404 0.77 -1.0 2,478,389 + Synpcc7942_2404 0.79 -0.2 2,478,419 + Synpcc7942_2404 0.83 +1.6 2,478,613 + -1.0 2,478,626 + +0.9 2,478,629 + -1.0 2,478,631 - -1.7 2,478,684 - -0.5 2,478,687 + +1.6 2,478,695 - +1.0 2,478,703 - -0.6 2,478,713 + -1.2 2,478,730 + +0.5 2,478,731 + +3.0 2,478,731 + -1.3 2,478,731 + -1.3 2,478,736 + +0.4 2,478,736 + -0.8 2,478,736 + +1.0 2,478,739 - -0.3 2,478,739 - +1.0 2,478,739 - +1.0 2,478,744 - -0.0 2,478,744 - -1.8 2,478,744 - -1.0 2,478,772 + Synpcc7942_2405 0.13 +2.6 2,478,794 + Synpcc7942_2405 0.22 +3.0 2,478,828 + Synpcc7942_2405 0.35 +0.3 2,478,881 - Synpcc7942_2405 0.56 -2.4 2,478,881 - Synpcc7942_2405 0.56 +1.5 2,478,913 + Synpcc7942_2405 0.68 +3.3 2,478,919 + Synpcc7942_2405 0.71 -0.5 2,478,921 - Synpcc7942_2405 0.71 -0.9 2,478,921 - Synpcc7942_2405 0.71 +1.8 2,478,924 + Synpcc7942_2405 0.73 +0.5 2,478,924 + Synpcc7942_2405 0.73 -1.2 2,478,929 - Synpcc7942_2405 0.75 -0.2 2,478,932 - -2.8 2,478,995 + +1.0
Or see this region's nucleotide sequence