Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ffs and panB are separated by 79 nucleotides panB and hemN overlap by 4 nucleotides hemN and ircA are separated by 39 nucleotides
Synpcc7942_R0047: ffs - SRP RNA, at 2,447,896 to 2,447,996
ffs
Synpcc7942_2381: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 2,448,076 to 2,448,882
panB
Synpcc7942_2382: hemN - coproporphyrinogen III oxidase, at 2,448,879 to 2,450,069
hemN
Synpcc7942_2383: ircA - Nucleotide binding protein, PINc, at 2,450,109 to 2,451,260
ircA
Position (kb)
2448
2449
2450
2451 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2447.883 kb on + strand at 2447.885 kb on - strand at 2448.002 kb on + strand at 2448.009 kb on + strand at 2448.009 kb on + strand at 2448.010 kb on - strand at 2448.041 kb on + strand at 2448.067 kb on + strand at 2448.876 kb on + strand at 2448.876 kb on + strand at 2448.876 kb on + strand at 2448.876 kb on + strand at 2448.876 kb on + strand at 2448.876 kb on + strand at 2448.876 kb on + strand at 2448.876 kb on + strand at 2448.876 kb on + strand at 2448.876 kb on + strand at 2448.878 kb on + strand at 2448.884 kb on - strand at 2448.884 kb on - strand at 2448.884 kb on - strand at 2448.884 kb on - strand at 2448.884 kb on - strand at 2448.884 kb on - strand at 2448.884 kb on - strand at 2448.884 kb on - strand at 2448.884 kb on - strand at 2448.884 kb on - strand at 2448.886 kb on + strand at 2448.895 kb on + strand at 2448.919 kb on + strand at 2448.919 kb on + strand at 2448.924 kb on + strand at 2448.927 kb on - strand at 2448.940 kb on + strand at 2448.946 kb on + strand at 2448.947 kb on - strand at 2448.948 kb on - strand at 2448.962 kb on + strand at 2448.974 kb on + strand at 2448.976 kb on - strand at 2449.033 kb on - strand, within hemN at 2449.058 kb on + strand, within hemN at 2449.059 kb on + strand at 2449.066 kb on - strand, within hemN at 2449.069 kb on - strand, within hemN at 2449.071 kb on - strand, within hemN at 2449.074 kb on + strand, within hemN at 2449.082 kb on - strand, within hemN at 2449.092 kb on + strand, within hemN at 2449.122 kb on + strand, within hemN at 2449.133 kb on + strand, within hemN at 2449.133 kb on + strand, within hemN at 2449.133 kb on + strand, within hemN at 2449.141 kb on - strand, within hemN at 2449.146 kb on - strand, within hemN at 2449.158 kb on - strand, within hemN at 2449.169 kb on + strand, within hemN at 2449.256 kb on + strand, within hemN at 2449.264 kb on - strand, within hemN at 2449.271 kb on - strand, within hemN at 2449.272 kb on + strand, within hemN at 2449.272 kb on + strand, within hemN at 2449.272 kb on + strand, within hemN at 2449.354 kb on - strand, within hemN at 2449.371 kb on + strand, within hemN at 2449.386 kb on + strand, within hemN at 2449.394 kb on - strand, within hemN at 2449.394 kb on - strand, within hemN at 2449.394 kb on - strand, within hemN at 2449.414 kb on + strand, within hemN at 2449.422 kb on - strand, within hemN at 2449.454 kb on - strand, within hemN at 2449.470 kb on - strand, within hemN at 2449.484 kb on - strand, within hemN at 2449.490 kb on + strand, within hemN at 2449.499 kb on + strand, within hemN at 2449.499 kb on + strand, within hemN at 2449.499 kb on + strand, within hemN at 2449.507 kb on - strand, within hemN at 2449.507 kb on - strand, within hemN at 2449.507 kb on - strand at 2449.507 kb on - strand, within hemN at 2449.522 kb on + strand, within hemN at 2449.627 kb on + strand, within hemN at 2449.656 kb on - strand, within hemN at 2449.656 kb on - strand, within hemN at 2449.657 kb on - strand, within hemN at 2449.679 kb on - strand, within hemN at 2449.693 kb on + strand, within hemN at 2449.704 kb on + strand, within hemN at 2449.704 kb on + strand, within hemN at 2449.712 kb on - strand, within hemN at 2449.718 kb on + strand, within hemN at 2449.718 kb on - strand, within hemN at 2449.722 kb on + strand, within hemN at 2449.743 kb on + strand, within hemN at 2449.805 kb on + strand, within hemN at 2449.812 kb on + strand, within hemN at 2449.825 kb on + strand, within hemN at 2449.848 kb on - strand, within hemN at 2449.886 kb on + strand, within hemN at 2449.894 kb on - strand, within hemN at 2449.956 kb on + strand at 2449.956 kb on + strand at 2449.966 kb on - strand at 2449.966 kb on - strand at 2450.005 kb on + strand at 2450.037 kb on - strand at 2450.085 kb on + strand at 2450.085 kb on + strand at 2450.093 kb on - strand at 2450.093 kb on - strand at 2450.093 kb on - strand at 2450.103 kb on - strand at 2450.124 kb on - strand at 2450.129 kb on - strand at 2450.129 kb on - strand at 2450.133 kb on + strand at 2450.148 kb on + strand at 2450.148 kb on + strand at 2450.156 kb on - strand at 2450.156 kb on - strand at 2450.171 kb on + strand at 2450.173 kb on - strand at 2450.189 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 2,447,883 + -1.8 2,447,885 - +0.4 2,448,002 + -0.8 2,448,009 + +1.3 2,448,009 + -0.0 2,448,010 - -0.0 2,448,041 + +1.0 2,448,067 + +1.0 2,448,876 + -0.8 2,448,876 + -0.7 2,448,876 + -0.8 2,448,876 + -0.9 2,448,876 + -1.1 2,448,876 + -0.5 2,448,876 + -0.9 2,448,876 + -1.6 2,448,876 + -0.0 2,448,876 + -0.0 2,448,878 + -1.1 2,448,884 - +0.2 2,448,884 - -0.5 2,448,884 - -0.4 2,448,884 - -1.2 2,448,884 - -1.7 2,448,884 - -0.0 2,448,884 - -1.4 2,448,884 - +1.0 2,448,884 - -1.0 2,448,884 - -0.3 2,448,886 + -1.2 2,448,895 + +1.0 2,448,919 + -0.2 2,448,919 + +0.1 2,448,924 + -0.6 2,448,927 - +1.6 2,448,940 + -0.0 2,448,946 + +2.6 2,448,947 - +2.6 2,448,948 - -0.8 2,448,962 + -0.1 2,448,974 + +1.6 2,448,976 - -0.8 2,449,033 - hemN Synpcc7942_2382 0.13 -1.4 2,449,058 + hemN Synpcc7942_2382 0.15 -1.8 2,449,059 + +2.0 2,449,066 - hemN Synpcc7942_2382 0.16 -0.1 2,449,069 - hemN Synpcc7942_2382 0.16 -0.7 2,449,071 - hemN Synpcc7942_2382 0.16 +0.3 2,449,074 + hemN Synpcc7942_2382 0.16 +0.9 2,449,082 - hemN Synpcc7942_2382 0.17 -0.5 2,449,092 + hemN Synpcc7942_2382 0.18 -0.9 2,449,122 + hemN Synpcc7942_2382 0.20 -0.9 2,449,133 + hemN Synpcc7942_2382 0.21 -0.1 2,449,133 + hemN Synpcc7942_2382 0.21 -1.0 2,449,133 + hemN Synpcc7942_2382 0.21 -0.6 2,449,141 - hemN Synpcc7942_2382 0.22 +0.7 2,449,146 - hemN Synpcc7942_2382 0.22 +0.5 2,449,158 - hemN Synpcc7942_2382 0.23 +1.0 2,449,169 + hemN Synpcc7942_2382 0.24 +1.0 2,449,256 + hemN Synpcc7942_2382 0.32 -2.2 2,449,264 - hemN Synpcc7942_2382 0.32 +1.4 2,449,271 - hemN Synpcc7942_2382 0.33 +0.4 2,449,272 + hemN Synpcc7942_2382 0.33 -0.4 2,449,272 + hemN Synpcc7942_2382 0.33 -0.1 2,449,272 + hemN Synpcc7942_2382 0.33 +0.2 2,449,354 - hemN Synpcc7942_2382 0.40 +1.7 2,449,371 + hemN Synpcc7942_2382 0.41 +0.4 2,449,386 + hemN Synpcc7942_2382 0.43 -0.9 2,449,394 - hemN Synpcc7942_2382 0.43 +1.4 2,449,394 - hemN Synpcc7942_2382 0.43 +1.0 2,449,394 - hemN Synpcc7942_2382 0.43 +2.1 2,449,414 + hemN Synpcc7942_2382 0.45 -0.7 2,449,422 - hemN Synpcc7942_2382 0.46 +0.7 2,449,454 - hemN Synpcc7942_2382 0.48 -1.1 2,449,470 - hemN Synpcc7942_2382 0.50 +2.0 2,449,484 - hemN Synpcc7942_2382 0.51 -0.9 2,449,490 + hemN Synpcc7942_2382 0.51 -1.8 2,449,499 + hemN Synpcc7942_2382 0.52 -0.3 2,449,499 + hemN Synpcc7942_2382 0.52 -0.2 2,449,499 + hemN Synpcc7942_2382 0.52 +1.6 2,449,507 - hemN Synpcc7942_2382 0.53 -0.4 2,449,507 - hemN Synpcc7942_2382 0.53 -0.6 2,449,507 - -0.8 2,449,507 - hemN Synpcc7942_2382 0.53 +0.7 2,449,522 + hemN Synpcc7942_2382 0.54 -1.0 2,449,627 + hemN Synpcc7942_2382 0.63 +1.6 2,449,656 - hemN Synpcc7942_2382 0.65 -0.2 2,449,656 - hemN Synpcc7942_2382 0.65 -0.6 2,449,657 - hemN Synpcc7942_2382 0.65 -0.1 2,449,679 - hemN Synpcc7942_2382 0.67 -1.0 2,449,693 + hemN Synpcc7942_2382 0.68 -0.6 2,449,704 + hemN Synpcc7942_2382 0.69 -0.7 2,449,704 + hemN Synpcc7942_2382 0.69 -1.3 2,449,712 - hemN Synpcc7942_2382 0.70 -0.1 2,449,718 + hemN Synpcc7942_2382 0.70 -0.6 2,449,718 - hemN Synpcc7942_2382 0.70 -1.3 2,449,722 + hemN Synpcc7942_2382 0.71 -0.6 2,449,743 + hemN Synpcc7942_2382 0.73 -1.6 2,449,805 + hemN Synpcc7942_2382 0.78 -1.5 2,449,812 + hemN Synpcc7942_2382 0.78 +0.3 2,449,825 + hemN Synpcc7942_2382 0.79 -0.6 2,449,848 - hemN Synpcc7942_2382 0.81 -1.9 2,449,886 + hemN Synpcc7942_2382 0.85 -1.5 2,449,894 - hemN Synpcc7942_2382 0.85 +1.3 2,449,956 + -0.3 2,449,956 + +1.0 2,449,966 - -0.0 2,449,966 - -1.2 2,450,005 + -3.1 2,450,037 - +0.1 2,450,085 + +1.0 2,450,085 + -0.5 2,450,093 - -0.5 2,450,093 - -0.6 2,450,093 - -1.0 2,450,103 - -0.0 2,450,124 - -0.8 2,450,129 - +1.5 2,450,129 - -0.1 2,450,133 + -0.1 2,450,148 + -0.4 2,450,148 + -0.3 2,450,156 - -2.1 2,450,156 - -0.2 2,450,171 + -0.8 2,450,173 - -0.6 2,450,189 - +1.0
Or see this region's nucleotide sequence