Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt idiA and dpsA are separated by 103 nucleotides dpsA and natD are separated by 46 nucleotides natD and Synpcc7942_2178 are separated by 20 nucleotides
Synpcc7942_2175: idiA - transport system substrate-binding protein, at 2,254,747 to 2,255,847
idiA
Synpcc7942_2176: dpsA - hypothetical protein, at 2,255,951 to 2,257,096
dpsA
Synpcc7942_2177: natD - integral membrane protein of the ABC-type Nat permease for neutral amino acids NatD, at 2,257,143 to 2,258,033
natD
Synpcc7942_2178: Synpcc7942_2178 - hypothetical protein, at 2,258,054 to 2,258,395
_2178
Position (kb)
2255
2256
2257
2258 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2254.958 kb on + strand, within idiA at 2254.958 kb on - strand, within idiA at 2254.958 kb on - strand, within idiA at 2254.960 kb on + strand, within idiA at 2255.009 kb on + strand, within idiA at 2255.167 kb on + strand, within idiA at 2255.194 kb on + strand, within idiA at 2255.202 kb on - strand, within idiA at 2255.202 kb on - strand, within idiA at 2255.234 kb on + strand, within idiA at 2255.242 kb on - strand, within idiA at 2255.272 kb on + strand at 2255.279 kb on - strand, within idiA at 2255.283 kb on + strand, within idiA at 2255.283 kb on + strand, within idiA at 2255.324 kb on - strand, within idiA at 2255.334 kb on - strand, within idiA at 2255.339 kb on + strand, within idiA at 2255.341 kb on + strand, within idiA at 2255.360 kb on - strand, within idiA at 2255.380 kb on - strand, within idiA at 2255.396 kb on + strand, within idiA at 2255.396 kb on + strand, within idiA at 2255.404 kb on - strand, within idiA at 2255.406 kb on + strand, within idiA at 2255.406 kb on + strand, within idiA at 2255.427 kb on - strand, within idiA at 2255.458 kb on - strand, within idiA at 2255.460 kb on + strand, within idiA at 2255.499 kb on - strand, within idiA at 2255.500 kb on + strand, within idiA at 2255.500 kb on + strand, within idiA at 2255.500 kb on + strand at 2255.513 kb on + strand at 2255.535 kb on - strand, within idiA at 2255.573 kb on + strand, within idiA at 2255.573 kb on + strand, within idiA at 2255.573 kb on + strand at 2255.581 kb on - strand at 2255.584 kb on + strand, within idiA at 2255.592 kb on - strand, within idiA at 2255.744 kb on - strand at 2255.764 kb on + strand at 2255.824 kb on + strand at 2255.833 kb on - strand at 2255.853 kb on - strand at 2255.900 kb on - strand at 2255.918 kb on + strand at 2255.918 kb on + strand at 2255.918 kb on + strand at 2255.926 kb on - strand at 2255.926 kb on - strand at 2255.926 kb on - strand at 2255.936 kb on - strand at 2255.943 kb on + strand at 2255.957 kb on - strand at 2255.958 kb on - strand at 2255.962 kb on - strand at 2255.963 kb on - strand at 2255.974 kb on - strand at 2255.999 kb on + strand at 2255.999 kb on + strand at 2256.001 kb on + strand at 2256.001 kb on + strand at 2256.020 kb on + strand at 2256.021 kb on + strand at 2256.029 kb on - strand at 2256.051 kb on - strand at 2256.064 kb on - strand at 2256.100 kb on + strand, within dpsA at 2256.108 kb on - strand, within dpsA at 2256.108 kb on - strand, within dpsA at 2256.108 kb on - strand, within dpsA at 2256.110 kb on + strand, within dpsA at 2256.113 kb on - strand, within dpsA at 2256.128 kb on + strand, within dpsA at 2256.130 kb on - strand, within dpsA at 2256.135 kb on + strand, within dpsA at 2256.135 kb on + strand, within dpsA at 2256.143 kb on - strand, within dpsA at 2256.150 kb on - strand, within dpsA at 2256.172 kb on + strand, within dpsA at 2256.180 kb on - strand, within dpsA at 2256.195 kb on + strand, within dpsA at 2256.213 kb on - strand, within dpsA at 2256.218 kb on - strand, within dpsA at 2256.232 kb on + strand, within dpsA at 2256.240 kb on - strand, within dpsA at 2256.260 kb on - strand, within dpsA at 2256.357 kb on + strand, within dpsA at 2256.360 kb on - strand, within dpsA at 2256.362 kb on + strand, within dpsA at 2256.365 kb on - strand, within dpsA at 2256.365 kb on - strand, within dpsA at 2256.406 kb on + strand, within dpsA at 2256.406 kb on + strand, within dpsA at 2256.437 kb on - strand, within dpsA at 2256.444 kb on - strand, within dpsA at 2256.475 kb on + strand, within dpsA at 2256.483 kb on - strand, within dpsA at 2256.483 kb on - strand, within dpsA at 2256.485 kb on - strand, within dpsA at 2256.527 kb on - strand, within dpsA at 2256.576 kb on - strand, within dpsA at 2256.578 kb on + strand at 2256.578 kb on + strand, within dpsA at 2256.578 kb on + strand at 2256.586 kb on + strand, within dpsA at 2256.586 kb on - strand, within dpsA at 2256.586 kb on - strand, within dpsA at 2256.586 kb on - strand, within dpsA at 2256.600 kb on + strand, within dpsA at 2256.600 kb on + strand, within dpsA at 2256.600 kb on + strand, within dpsA at 2256.600 kb on + strand, within dpsA at 2256.600 kb on + strand, within dpsA at 2256.608 kb on - strand, within dpsA at 2256.608 kb on - strand, within dpsA at 2256.608 kb on - strand, within dpsA at 2256.608 kb on - strand, within dpsA at 2256.609 kb on - strand, within dpsA at 2256.615 kb on + strand, within dpsA at 2256.615 kb on + strand, within dpsA at 2256.628 kb on - strand, within dpsA at 2256.630 kb on - strand, within dpsA at 2256.646 kb on + strand, within dpsA at 2256.652 kb on - strand, within dpsA at 2256.653 kb on - strand, within dpsA at 2256.676 kb on - strand, within dpsA at 2256.728 kb on - strand, within dpsA at 2256.800 kb on + strand, within dpsA at 2256.818 kb on + strand, within dpsA at 2256.839 kb on - strand, within dpsA at 2256.874 kb on - strand, within dpsA at 2256.887 kb on + strand, within dpsA at 2256.890 kb on - strand, within dpsA at 2256.890 kb on - strand, within dpsA at 2256.915 kb on + strand, within dpsA at 2256.972 kb on - strand, within dpsA at 2256.974 kb on - strand, within dpsA at 2256.991 kb on - strand at 2256.991 kb on - strand at 2257.000 kb on + strand at 2257.008 kb on - strand at 2257.042 kb on + strand at 2257.050 kb on - strand at 2257.053 kb on + strand at 2257.064 kb on + strand at 2257.072 kb on - strand at 2257.131 kb on + strand at 2257.138 kb on - strand at 2257.167 kb on + strand at 2257.167 kb on + strand at 2257.168 kb on + strand at 2257.168 kb on + strand at 2257.176 kb on - strand at 2257.177 kb on + strand at 2257.185 kb on - strand at 2257.197 kb on + strand at 2257.207 kb on - strand at 2257.224 kb on + strand at 2257.232 kb on - strand, within natD at 2257.232 kb on - strand, within natD at 2257.232 kb on - strand, within natD at 2257.320 kb on - strand, within natD at 2257.339 kb on + strand, within natD at 2257.347 kb on - strand, within natD at 2257.351 kb on + strand, within natD at 2257.351 kb on + strand, within natD at 2257.359 kb on - strand, within natD at 2257.359 kb on - strand, within natD at 2257.359 kb on - strand, within natD at 2257.420 kb on + strand at 2257.424 kb on - strand, within natD at 2257.474 kb on + strand, within natD at 2257.474 kb on + strand, within natD at 2257.474 kb on + strand, within natD at 2257.482 kb on - strand, within natD at 2257.560 kb on + strand, within natD at 2257.560 kb on + strand, within natD at 2257.561 kb on + strand, within natD at 2257.610 kb on + strand, within natD at 2257.640 kb on + strand, within natD at 2257.663 kb on - strand, within natD at 2257.663 kb on - strand, within natD at 2257.667 kb on + strand, within natD at 2257.667 kb on + strand, within natD at 2257.702 kb on + strand, within natD at 2257.710 kb on - strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.712 kb on + strand, within natD at 2257.720 kb on - strand, within natD at 2257.720 kb on - strand, within natD at 2257.720 kb on - strand, within natD at 2257.720 kb on - strand, within natD at 2257.720 kb on - strand, within natD at 2257.735 kb on + strand, within natD at 2257.768 kb on + strand, within natD at 2257.768 kb on + strand, within natD at 2257.798 kb on - strand, within natD at 2257.873 kb on + strand, within natD at 2257.880 kb on - strand, within natD at 2257.883 kb on + strand, within natD at 2257.883 kb on + strand, within natD at 2257.891 kb on - strand, within natD at 2257.891 kb on - strand, within natD at 2257.895 kb on + strand, within natD at 2257.931 kb on + strand, within natD at 2258.016 kb on + strand at 2258.019 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 2,254,958 + idiA Synpcc7942_2175 0.19 +1.0 2,254,958 - idiA Synpcc7942_2175 0.19 +2.0 2,254,958 - idiA Synpcc7942_2175 0.19 -1.4 2,254,960 + idiA Synpcc7942_2175 0.19 -0.4 2,255,009 + idiA Synpcc7942_2175 0.24 -1.2 2,255,167 + idiA Synpcc7942_2175 0.38 -0.7 2,255,194 + idiA Synpcc7942_2175 0.41 -0.7 2,255,202 - idiA Synpcc7942_2175 0.41 +1.3 2,255,202 - idiA Synpcc7942_2175 0.41 -0.2 2,255,234 + idiA Synpcc7942_2175 0.44 +0.0 2,255,242 - idiA Synpcc7942_2175 0.45 +2.0 2,255,272 + +1.0 2,255,279 - idiA Synpcc7942_2175 0.48 +0.0 2,255,283 + idiA Synpcc7942_2175 0.49 -1.2 2,255,283 + idiA Synpcc7942_2175 0.49 -0.9 2,255,324 - idiA Synpcc7942_2175 0.52 +0.2 2,255,334 - idiA Synpcc7942_2175 0.53 -0.3 2,255,339 + idiA Synpcc7942_2175 0.54 +0.0 2,255,341 + idiA Synpcc7942_2175 0.54 -0.6 2,255,360 - idiA Synpcc7942_2175 0.56 +0.0 2,255,380 - idiA Synpcc7942_2175 0.57 -0.4 2,255,396 + idiA Synpcc7942_2175 0.59 +0.0 2,255,396 + idiA Synpcc7942_2175 0.59 +2.0 2,255,404 - idiA Synpcc7942_2175 0.60 +0.0 2,255,406 + idiA Synpcc7942_2175 0.60 -0.3 2,255,406 + idiA Synpcc7942_2175 0.60 +0.4 2,255,427 - idiA Synpcc7942_2175 0.62 -1.0 2,255,458 - idiA Synpcc7942_2175 0.65 -0.2 2,255,460 + idiA Synpcc7942_2175 0.65 +1.0 2,255,499 - idiA Synpcc7942_2175 0.68 -1.0 2,255,500 + idiA Synpcc7942_2175 0.68 +1.0 2,255,500 + idiA Synpcc7942_2175 0.68 +0.6 2,255,500 + +2.0 2,255,513 + +1.0 2,255,535 - idiA Synpcc7942_2175 0.72 -0.8 2,255,573 + idiA Synpcc7942_2175 0.75 +1.0 2,255,573 + idiA Synpcc7942_2175 0.75 -2.4 2,255,573 + -1.1 2,255,581 - -1.0 2,255,584 + idiA Synpcc7942_2175 0.76 -0.2 2,255,592 - idiA Synpcc7942_2175 0.77 +1.0 2,255,744 - -1.0 2,255,764 + -1.4 2,255,824 + -1.0 2,255,833 - -0.6 2,255,853 - +0.0 2,255,900 - +0.0 2,255,918 + +1.9 2,255,918 + -1.7 2,255,918 + -0.6 2,255,926 - +1.0 2,255,926 - -0.7 2,255,926 - +0.4 2,255,936 - +2.0 2,255,943 + -2.3 2,255,957 - -0.3 2,255,958 - -1.5 2,255,962 - +0.2 2,255,963 - -0.7 2,255,974 - +1.0 2,255,999 + -0.6 2,255,999 + +0.0 2,256,001 + +1.0 2,256,001 + -1.0 2,256,020 + -1.0 2,256,021 + +0.0 2,256,029 - -0.8 2,256,051 - +2.0 2,256,064 - -0.7 2,256,100 + dpsA Synpcc7942_2176 0.13 -0.1 2,256,108 - dpsA Synpcc7942_2176 0.14 -1.3 2,256,108 - dpsA Synpcc7942_2176 0.14 -1.1 2,256,108 - dpsA Synpcc7942_2176 0.14 -0.3 2,256,110 + dpsA Synpcc7942_2176 0.14 -1.0 2,256,113 - dpsA Synpcc7942_2176 0.14 -1.9 2,256,128 + dpsA Synpcc7942_2176 0.15 -1.2 2,256,130 - dpsA Synpcc7942_2176 0.16 -0.5 2,256,135 + dpsA Synpcc7942_2176 0.16 -1.8 2,256,135 + dpsA Synpcc7942_2176 0.16 -0.8 2,256,143 - dpsA Synpcc7942_2176 0.17 -1.2 2,256,150 - dpsA Synpcc7942_2176 0.17 -0.1 2,256,172 + dpsA Synpcc7942_2176 0.19 +2.6 2,256,180 - dpsA Synpcc7942_2176 0.20 -0.5 2,256,195 + dpsA Synpcc7942_2176 0.21 +0.3 2,256,213 - dpsA Synpcc7942_2176 0.23 -0.6 2,256,218 - dpsA Synpcc7942_2176 0.23 -2.4 2,256,232 + dpsA Synpcc7942_2176 0.25 -0.9 2,256,240 - dpsA Synpcc7942_2176 0.25 -1.5 2,256,260 - dpsA Synpcc7942_2176 0.27 +1.4 2,256,357 + dpsA Synpcc7942_2176 0.35 -3.0 2,256,360 - dpsA Synpcc7942_2176 0.36 -1.1 2,256,362 + dpsA Synpcc7942_2176 0.36 -2.5 2,256,365 - dpsA Synpcc7942_2176 0.36 -1.3 2,256,365 - dpsA Synpcc7942_2176 0.36 -0.6 2,256,406 + dpsA Synpcc7942_2176 0.40 -1.2 2,256,406 + dpsA Synpcc7942_2176 0.40 -1.9 2,256,437 - dpsA Synpcc7942_2176 0.42 +0.0 2,256,444 - dpsA Synpcc7942_2176 0.43 +0.0 2,256,475 + dpsA Synpcc7942_2176 0.46 -0.6 2,256,483 - dpsA Synpcc7942_2176 0.46 -1.9 2,256,483 - dpsA Synpcc7942_2176 0.46 -0.9 2,256,485 - dpsA Synpcc7942_2176 0.47 -0.7 2,256,527 - dpsA Synpcc7942_2176 0.50 +1.0 2,256,576 - dpsA Synpcc7942_2176 0.55 -1.0 2,256,578 + -0.4 2,256,578 + dpsA Synpcc7942_2176 0.55 +2.6 2,256,578 + -1.2 2,256,586 + dpsA Synpcc7942_2176 0.55 -0.8 2,256,586 - dpsA Synpcc7942_2176 0.55 -0.0 2,256,586 - dpsA Synpcc7942_2176 0.55 -2.3 2,256,586 - dpsA Synpcc7942_2176 0.55 +0.4 2,256,600 + dpsA Synpcc7942_2176 0.57 +0.3 2,256,600 + dpsA Synpcc7942_2176 0.57 +0.4 2,256,600 + dpsA Synpcc7942_2176 0.57 -0.8 2,256,600 + dpsA Synpcc7942_2176 0.57 +1.0 2,256,600 + dpsA Synpcc7942_2176 0.57 -1.3 2,256,608 - dpsA Synpcc7942_2176 0.57 +0.6 2,256,608 - dpsA Synpcc7942_2176 0.57 -0.6 2,256,608 - dpsA Synpcc7942_2176 0.57 -2.4 2,256,608 - dpsA Synpcc7942_2176 0.57 -0.4 2,256,609 - dpsA Synpcc7942_2176 0.57 -0.1 2,256,615 + dpsA Synpcc7942_2176 0.58 -1.1 2,256,615 + dpsA Synpcc7942_2176 0.58 -0.3 2,256,628 - dpsA Synpcc7942_2176 0.59 -1.2 2,256,630 - dpsA Synpcc7942_2176 0.59 +0.0 2,256,646 + dpsA Synpcc7942_2176 0.61 -0.6 2,256,652 - dpsA Synpcc7942_2176 0.61 -0.5 2,256,653 - dpsA Synpcc7942_2176 0.61 -2.0 2,256,676 - dpsA Synpcc7942_2176 0.63 -0.9 2,256,728 - dpsA Synpcc7942_2176 0.68 -0.2 2,256,800 + dpsA Synpcc7942_2176 0.74 -1.3 2,256,818 + dpsA Synpcc7942_2176 0.76 -0.6 2,256,839 - dpsA Synpcc7942_2176 0.77 -1.8 2,256,874 - dpsA Synpcc7942_2176 0.81 +1.1 2,256,887 + dpsA Synpcc7942_2176 0.82 -1.3 2,256,890 - dpsA Synpcc7942_2176 0.82 +0.0 2,256,890 - dpsA Synpcc7942_2176 0.82 -0.5 2,256,915 + dpsA Synpcc7942_2176 0.84 -0.4 2,256,972 - dpsA Synpcc7942_2176 0.89 -1.7 2,256,974 - dpsA Synpcc7942_2176 0.89 -1.9 2,256,991 - -0.8 2,256,991 - -3.2 2,257,000 + -1.0 2,257,008 - +0.0 2,257,042 + -0.2 2,257,050 - +1.0 2,257,053 + -0.1 2,257,064 + +0.8 2,257,072 - +1.0 2,257,131 + +0.5 2,257,138 - -2.2 2,257,167 + -0.9 2,257,167 + -0.5 2,257,168 + +1.0 2,257,168 + -0.6 2,257,176 - +0.4 2,257,177 + -0.6 2,257,185 - +1.0 2,257,197 + -0.3 2,257,207 - +1.0 2,257,224 + -1.3 2,257,232 - natD Synpcc7942_2177 0.10 -0.1 2,257,232 - natD Synpcc7942_2177 0.10 +0.0 2,257,232 - natD Synpcc7942_2177 0.10 +0.6 2,257,320 - natD Synpcc7942_2177 0.20 -0.9 2,257,339 + natD Synpcc7942_2177 0.22 +0.2 2,257,347 - natD Synpcc7942_2177 0.23 +0.0 2,257,351 + natD Synpcc7942_2177 0.23 -0.7 2,257,351 + natD Synpcc7942_2177 0.23 -1.4 2,257,359 - natD Synpcc7942_2177 0.24 +0.0 2,257,359 - natD Synpcc7942_2177 0.24 -0.4 2,257,359 - natD Synpcc7942_2177 0.24 -0.7 2,257,420 + +0.6 2,257,424 - natD Synpcc7942_2177 0.32 -1.0 2,257,474 + natD Synpcc7942_2177 0.37 -1.7 2,257,474 + natD Synpcc7942_2177 0.37 -1.0 2,257,474 + natD Synpcc7942_2177 0.37 -0.6 2,257,482 - natD Synpcc7942_2177 0.38 -2.2 2,257,560 + natD Synpcc7942_2177 0.47 -1.3 2,257,560 + natD Synpcc7942_2177 0.47 +0.4 2,257,561 + natD Synpcc7942_2177 0.47 -0.8 2,257,610 + natD Synpcc7942_2177 0.52 -0.6 2,257,640 + natD Synpcc7942_2177 0.56 -0.2 2,257,663 - natD Synpcc7942_2177 0.58 +3.0 2,257,663 - natD Synpcc7942_2177 0.58 -1.2 2,257,667 + natD Synpcc7942_2177 0.59 -0.3 2,257,667 + natD Synpcc7942_2177 0.59 -0.4 2,257,702 + natD Synpcc7942_2177 0.63 -0.6 2,257,710 - natD Synpcc7942_2177 0.64 +0.1 2,257,712 + natD Synpcc7942_2177 0.64 -2.0 2,257,712 + natD Synpcc7942_2177 0.64 -0.1 2,257,712 + natD Synpcc7942_2177 0.64 -0.3 2,257,712 + natD Synpcc7942_2177 0.64 -2.2 2,257,712 + natD Synpcc7942_2177 0.64 -0.2 2,257,712 + natD Synpcc7942_2177 0.64 -1.3 2,257,712 + natD Synpcc7942_2177 0.64 -1.1 2,257,712 + natD Synpcc7942_2177 0.64 +1.0 2,257,712 + natD Synpcc7942_2177 0.64 -0.6 2,257,720 - natD Synpcc7942_2177 0.65 +0.3 2,257,720 - natD Synpcc7942_2177 0.65 -1.4 2,257,720 - natD Synpcc7942_2177 0.65 +2.5 2,257,720 - natD Synpcc7942_2177 0.65 -0.8 2,257,720 - natD Synpcc7942_2177 0.65 +0.7 2,257,735 + natD Synpcc7942_2177 0.66 +1.4 2,257,768 + natD Synpcc7942_2177 0.70 -0.7 2,257,768 + natD Synpcc7942_2177 0.70 -0.8 2,257,798 - natD Synpcc7942_2177 0.74 +1.0 2,257,873 + natD Synpcc7942_2177 0.82 -0.5 2,257,880 - natD Synpcc7942_2177 0.83 +1.0 2,257,883 + natD Synpcc7942_2177 0.83 +0.0 2,257,883 + natD Synpcc7942_2177 0.83 +1.0 2,257,891 - natD Synpcc7942_2177 0.84 -0.5 2,257,891 - natD Synpcc7942_2177 0.84 -0.7 2,257,895 + natD Synpcc7942_2177 0.84 -1.4 2,257,931 + natD Synpcc7942_2177 0.88 +0.6 2,258,016 + -0.2 2,258,019 - -0.8
Or see this region's nucleotide sequence