Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemB and trxA are separated by 169 nucleotides trxA and Synpcc7942_1794 are separated by 4 nucleotides Synpcc7942_1794 and smpB are separated by 32 nucleotides smpB and Synpcc7942_1796 are separated by 12 nucleotides Synpcc7942_1796 and Synpcc7942_1797 are separated by 123 nucleotides
Synpcc7942_1792: hemB - delta-aminolevulinic acid dehydratase, at 1,863,290 to 1,864,270
hemB
Synpcc7942_1793: trxA - thioredoxin, at 1,864,440 to 1,864,763
trxA
Synpcc7942_1794: Synpcc7942_1794 - succinyldiaminopimelate transaminase, at 1,864,768 to 1,865,946
_1794
Synpcc7942_1795: smpB - SsrA-binding protein, at 1,865,979 to 1,866,443
smpB
Synpcc7942_1796: Synpcc7942_1796 - hypothetical protein, at 1,866,456 to 1,866,749
_1796
Synpcc7942_1797: Synpcc7942_1797 - hypothetical protein, at 1,866,873 to 1,867,376
_1797
Position (kb)
1864
1865
1866 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1864.264 kb on + strand at 1864.272 kb on - strand at 1864.272 kb on - strand at 1864.282 kb on - strand at 1864.318 kb on - strand at 1864.318 kb on - strand at 1864.318 kb on - strand at 1864.318 kb on - strand at 1864.318 kb on - strand at 1864.318 kb on - strand at 1864.318 kb on - strand at 1864.336 kb on + strand at 1864.341 kb on - strand at 1864.379 kb on - strand at 1864.540 kb on - strand, within trxA at 1864.573 kb on - strand, within trxA at 1864.575 kb on + strand, within trxA at 1864.580 kb on + strand, within trxA at 1864.580 kb on + strand, within trxA at 1864.583 kb on - strand, within trxA at 1864.602 kb on + strand, within trxA at 1864.656 kb on - strand, within trxA at 1864.656 kb on - strand, within trxA at 1864.679 kb on + strand, within trxA at 1864.697 kb on + strand, within trxA at 1864.726 kb on - strand, within trxA at 1864.809 kb on - strand at 1864.844 kb on - strand at 1864.851 kb on - strand at 1864.862 kb on + strand at 1864.900 kb on - strand, within Synpcc7942_1794 at 1864.904 kb on - strand, within Synpcc7942_1794 at 1864.905 kb on - strand, within Synpcc7942_1794 at 1864.907 kb on + strand, within Synpcc7942_1794 at 1864.939 kb on + strand, within Synpcc7942_1794 at 1864.948 kb on - strand, within Synpcc7942_1794 at 1864.957 kb on + strand, within Synpcc7942_1794 at 1865.023 kb on - strand, within Synpcc7942_1794 at 1865.047 kb on - strand, within Synpcc7942_1794 at 1865.075 kb on + strand, within Synpcc7942_1794 at 1865.075 kb on - strand, within Synpcc7942_1794 at 1865.082 kb on + strand, within Synpcc7942_1794 at 1865.083 kb on - strand, within Synpcc7942_1794 at 1865.112 kb on + strand, within Synpcc7942_1794 at 1865.126 kb on + strand, within Synpcc7942_1794 at 1865.136 kb on + strand, within Synpcc7942_1794 at 1865.136 kb on + strand, within Synpcc7942_1794 at 1865.143 kb on + strand, within Synpcc7942_1794 at 1865.143 kb on + strand, within Synpcc7942_1794 at 1865.148 kb on + strand, within Synpcc7942_1794 at 1865.151 kb on - strand, within Synpcc7942_1794 at 1865.157 kb on + strand, within Synpcc7942_1794 at 1865.167 kb on + strand, within Synpcc7942_1794 at 1865.167 kb on + strand, within Synpcc7942_1794 at 1865.246 kb on - strand, within Synpcc7942_1794 at 1865.284 kb on + strand, within Synpcc7942_1794 at 1865.388 kb on - strand, within Synpcc7942_1794 at 1865.399 kb on - strand, within Synpcc7942_1794 at 1865.417 kb on + strand, within Synpcc7942_1794 at 1865.425 kb on - strand, within Synpcc7942_1794 at 1865.427 kb on + strand, within Synpcc7942_1794 at 1865.474 kb on + strand, within Synpcc7942_1794 at 1865.534 kb on + strand, within Synpcc7942_1794 at 1865.549 kb on - strand, within Synpcc7942_1794 at 1865.551 kb on + strand, within Synpcc7942_1794 at 1865.552 kb on + strand, within Synpcc7942_1794 at 1865.552 kb on - strand, within Synpcc7942_1794 at 1865.570 kb on - strand, within Synpcc7942_1794 at 1865.599 kb on + strand, within Synpcc7942_1794 at 1865.640 kb on - strand, within Synpcc7942_1794 at 1865.656 kb on + strand, within Synpcc7942_1794 at 1865.690 kb on + strand, within Synpcc7942_1794 at 1865.691 kb on - strand, within Synpcc7942_1794 at 1865.695 kb on + strand, within Synpcc7942_1794 at 1865.727 kb on - strand, within Synpcc7942_1794 at 1865.739 kb on - strand, within Synpcc7942_1794 at 1865.745 kb on - strand, within Synpcc7942_1794 at 1865.745 kb on - strand, within Synpcc7942_1794 at 1865.748 kb on - strand, within Synpcc7942_1794 at 1865.753 kb on - strand, within Synpcc7942_1794 at 1865.783 kb on + strand, within Synpcc7942_1794 at 1865.822 kb on - strand, within Synpcc7942_1794 at 1865.833 kb on - strand at 1865.878 kb on + strand at 1865.887 kb on - strand at 1865.892 kb on - strand at 1865.892 kb on - strand at 1865.909 kb on - strand at 1865.926 kb on + strand at 1865.929 kb on - strand at 1865.931 kb on - strand at 1865.947 kb on + strand at 1865.962 kb on + strand at 1865.962 kb on - strand at 1865.967 kb on + strand at 1865.967 kb on + strand at 1865.970 kb on - strand at 1865.975 kb on - strand at 1865.977 kb on + strand at 1865.985 kb on - strand at 1865.986 kb on + strand at 1866.418 kb on + strand at 1866.449 kb on + strand at 1866.449 kb on + strand at 1866.452 kb on - strand at 1866.481 kb on + strand at 1866.481 kb on + strand at 1866.487 kb on - strand, within Synpcc7942_1796 at 1866.495 kb on - strand, within Synpcc7942_1796 at 1866.512 kb on + strand, within Synpcc7942_1796 at 1866.512 kb on + strand, within Synpcc7942_1796 at 1866.512 kb on + strand, within Synpcc7942_1796 at 1866.586 kb on + strand, within Synpcc7942_1796 at 1866.586 kb on + strand, within Synpcc7942_1796 at 1866.594 kb on + strand, within Synpcc7942_1796 at 1866.594 kb on - strand, within Synpcc7942_1796 at 1866.670 kb on - strand, within Synpcc7942_1796 at 1866.672 kb on + strand, within Synpcc7942_1796 at 1866.672 kb on + strand, within Synpcc7942_1796 at 1866.680 kb on - strand, within Synpcc7942_1796 at 1866.723 kb on + strand at 1866.741 kb on + strand at 1866.741 kb on + strand at 1866.746 kb on + strand at 1866.746 kb on + strand at 1866.746 kb on + strand at 1866.751 kb on + strand at 1866.751 kb on + strand at 1866.753 kb on - strand at 1866.754 kb on - strand at 1866.754 kb on - strand at 1866.786 kb on + strand at 1866.794 kb on - strand at 1866.794 kb on - strand at 1866.794 kb on - strand at 1866.808 kb on + strand at 1866.811 kb on - strand at 1866.813 kb on + strand at 1866.816 kb on - strand at 1866.816 kb on - strand at 1866.816 kb on - strand at 1866.821 kb on - strand at 1866.821 kb on - strand at 1866.822 kb on + strand at 1866.869 kb on + strand at 1866.869 kb on + strand at 1866.870 kb on + strand at 1866.877 kb on - strand at 1866.877 kb on - strand at 1866.877 kb on - strand at 1866.879 kb on + strand at 1866.879 kb on + strand at 1866.879 kb on + strand at 1866.879 kb on + strand at 1866.880 kb on + strand at 1866.880 kb on + strand at 1866.887 kb on - strand at 1866.887 kb on - strand at 1866.887 kb on - strand at 1866.887 kb on - strand at 1866.925 kb on + strand, within Synpcc7942_1797 at 1866.943 kb on + strand, within Synpcc7942_1797
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 1,864,264 + +0.6 1,864,272 - -0.9 1,864,272 - -1.1 1,864,282 - -1.0 1,864,318 - -0.4 1,864,318 - +1.0 1,864,318 - -1.3 1,864,318 - -0.1 1,864,318 - +0.2 1,864,318 - -0.3 1,864,318 - -0.6 1,864,336 + -0.9 1,864,341 - -1.1 1,864,379 - -0.4 1,864,540 - trxA Synpcc7942_1793 0.31 +1.0 1,864,573 - trxA Synpcc7942_1793 0.41 +1.0 1,864,575 + trxA Synpcc7942_1793 0.42 +1.0 1,864,580 + trxA Synpcc7942_1793 0.43 -0.0 1,864,580 + trxA Synpcc7942_1793 0.43 +1.0 1,864,583 - trxA Synpcc7942_1793 0.44 -0.3 1,864,602 + trxA Synpcc7942_1793 0.50 -1.0 1,864,656 - trxA Synpcc7942_1793 0.67 -0.6 1,864,656 - trxA Synpcc7942_1793 0.67 +1.0 1,864,679 + trxA Synpcc7942_1793 0.74 -0.0 1,864,697 + trxA Synpcc7942_1793 0.79 +1.0 1,864,726 - trxA Synpcc7942_1793 0.88 -0.6 1,864,809 - -1.6 1,864,844 - +1.0 1,864,851 - +0.4 1,864,862 + -2.7 1,864,900 - Synpcc7942_1794 0.11 -0.0 1,864,904 - Synpcc7942_1794 0.12 +2.6 1,864,905 - Synpcc7942_1794 0.12 -1.7 1,864,907 + Synpcc7942_1794 0.12 -2.3 1,864,939 + Synpcc7942_1794 0.15 +1.0 1,864,948 - Synpcc7942_1794 0.15 +1.0 1,864,957 + Synpcc7942_1794 0.16 -0.0 1,865,023 - Synpcc7942_1794 0.22 +1.4 1,865,047 - Synpcc7942_1794 0.24 -0.6 1,865,075 + Synpcc7942_1794 0.26 -0.0 1,865,075 - Synpcc7942_1794 0.26 -1.0 1,865,082 + Synpcc7942_1794 0.27 +2.0 1,865,083 - Synpcc7942_1794 0.27 -0.6 1,865,112 + Synpcc7942_1794 0.29 -1.0 1,865,126 + Synpcc7942_1794 0.30 -1.0 1,865,136 + Synpcc7942_1794 0.31 +1.0 1,865,136 + Synpcc7942_1794 0.31 +1.0 1,865,143 + Synpcc7942_1794 0.32 -0.6 1,865,143 + Synpcc7942_1794 0.32 -0.0 1,865,148 + Synpcc7942_1794 0.32 -0.6 1,865,151 - Synpcc7942_1794 0.32 -0.0 1,865,157 + Synpcc7942_1794 0.33 -2.5 1,865,167 + Synpcc7942_1794 0.34 -1.3 1,865,167 + Synpcc7942_1794 0.34 +2.0 1,865,246 - Synpcc7942_1794 0.41 -0.6 1,865,284 + Synpcc7942_1794 0.44 -0.7 1,865,388 - Synpcc7942_1794 0.53 +1.0 1,865,399 - Synpcc7942_1794 0.54 +0.4 1,865,417 + Synpcc7942_1794 0.55 +1.0 1,865,425 - Synpcc7942_1794 0.56 -1.9 1,865,427 + Synpcc7942_1794 0.56 -0.0 1,865,474 + Synpcc7942_1794 0.60 +1.0 1,865,534 + Synpcc7942_1794 0.65 +0.1 1,865,549 - Synpcc7942_1794 0.66 -0.6 1,865,551 + Synpcc7942_1794 0.66 -0.8 1,865,552 + Synpcc7942_1794 0.66 -0.8 1,865,552 - Synpcc7942_1794 0.66 +1.0 1,865,570 - Synpcc7942_1794 0.68 -1.8 1,865,599 + Synpcc7942_1794 0.70 +1.0 1,865,640 - Synpcc7942_1794 0.74 -0.0 1,865,656 + Synpcc7942_1794 0.75 -0.0 1,865,690 + Synpcc7942_1794 0.78 -2.2 1,865,691 - Synpcc7942_1794 0.78 -4.0 1,865,695 + Synpcc7942_1794 0.79 +1.0 1,865,727 - Synpcc7942_1794 0.81 -1.0 1,865,739 - Synpcc7942_1794 0.82 +1.0 1,865,745 - Synpcc7942_1794 0.83 +1.0 1,865,745 - Synpcc7942_1794 0.83 +1.0 1,865,748 - Synpcc7942_1794 0.83 +1.0 1,865,753 - Synpcc7942_1794 0.84 +1.0 1,865,783 + Synpcc7942_1794 0.86 +1.0 1,865,822 - Synpcc7942_1794 0.89 +1.0 1,865,833 - +2.0 1,865,878 + +1.0 1,865,887 - +1.0 1,865,892 - +1.0 1,865,892 - +1.4 1,865,909 - -1.0 1,865,926 + +2.0 1,865,929 - -3.0 1,865,931 - +0.2 1,865,947 + -0.8 1,865,962 + -0.4 1,865,962 - -0.7 1,865,967 + -2.6 1,865,967 + -1.6 1,865,970 - -0.9 1,865,975 - -1.0 1,865,977 + -2.2 1,865,985 - -0.1 1,865,986 + -1.2 1,866,418 + -0.8 1,866,449 + -0.0 1,866,449 + +1.0 1,866,452 - +1.0 1,866,481 + -0.0 1,866,481 + -2.8 1,866,487 - Synpcc7942_1796 0.11 -1.3 1,866,495 - Synpcc7942_1796 0.13 -0.8 1,866,512 + Synpcc7942_1796 0.19 -0.0 1,866,512 + Synpcc7942_1796 0.19 -1.4 1,866,512 + Synpcc7942_1796 0.19 -0.8 1,866,586 + Synpcc7942_1796 0.44 -0.7 1,866,586 + Synpcc7942_1796 0.44 -0.6 1,866,594 + Synpcc7942_1796 0.47 -0.9 1,866,594 - Synpcc7942_1796 0.47 -1.0 1,866,670 - Synpcc7942_1796 0.73 -0.7 1,866,672 + Synpcc7942_1796 0.73 -1.5 1,866,672 + Synpcc7942_1796 0.73 +1.4 1,866,680 - Synpcc7942_1796 0.76 -0.6 1,866,723 + +1.4 1,866,741 + -1.2 1,866,741 + -1.0 1,866,746 + -0.7 1,866,746 + -1.7 1,866,746 + -1.3 1,866,751 + -0.0 1,866,751 + -1.0 1,866,753 - -0.0 1,866,754 - -0.9 1,866,754 - -1.0 1,866,786 + -0.5 1,866,794 - +2.0 1,866,794 - -1.2 1,866,794 - +1.0 1,866,808 + +1.0 1,866,811 - -0.6 1,866,813 + -0.7 1,866,816 - -0.5 1,866,816 - -0.0 1,866,816 - -1.6 1,866,821 - +1.0 1,866,821 - -0.2 1,866,822 + +0.6 1,866,869 + +0.4 1,866,869 + +0.2 1,866,870 + -1.3 1,866,877 - -1.6 1,866,877 - -0.4 1,866,877 - +1.2 1,866,879 + -0.2 1,866,879 + +1.0 1,866,879 + -1.4 1,866,879 + -1.6 1,866,880 + +0.4 1,866,880 + -0.6 1,866,887 - -0.5 1,866,887 - +1.0 1,866,887 - -0.4 1,866,887 - -2.7 1,866,925 + Synpcc7942_1797 0.10 +1.4 1,866,943 + Synpcc7942_1797 0.14 +1.0
Or see this region's nucleotide sequence